Characterization of human skin-derived mesenchymal stem cell proliferation rate in different growth conditions

Una Riekstina, Ruta Muceniece, Inese Cakstina, Indrikis Muiznieks, Janis Ancans

Research output: Contribution to journalArticlepeer-review

82 Citations (Scopus)

Abstract

This study investigated conditions for optimal in vitro propagation of human skin-derived mesenchymal stem cells (S-MSC). Forty primary skin-derived precursor cell (SKP) cultures were established from both male and female donors (age 29-65 years) and eight of them were randomly selected for in-depth characterization. Effects of basic fibroblast growth factor (FGF-2), epidermal growth factor (EGF), leukemia inhibiting factor (LIF) and dibutyryl-cyclic adenosine monophosphate (db-cAMP) on S-MSC proliferation were investigated. Primary SKP cultures were >95% homogenous for CD90, CD73, and CD105 marker expression enabling to classify these cells as S-MSC. FGF-2 dose-dependent stimulation was observed in low serum medium only, whereas EGF neither stimulated S-MSC proliferation nor potentates the effect of FGF-2. Pronounced donor to donor differences among S-MSC cultures were observed in 3-day proliferation assay. This study demonstrates that homogenous S-MSC populations can be reproducibly isolated from individual donors of different age. Optimal cell culture conditions for in vitro propagation of S-MSC are B27 supplemented or low serum media with FGF-2 (4 ng/ml). EGF and LIF as well as db-cAMP are dispensable for S-MSC proliferation.

Original languageEnglish
Pages (from-to)153-162
Number of pages10
JournalCytotechnology
Volume58
Issue number3
DOIs
Publication statusPublished - Nov 2008
Externally publishedYes

Keywords*

  • Db-cAMP
  • EGF
  • FACS
  • FBS
  • FGF-2
  • Proliferation
  • Skin-derived mesenchymal stem cells (S-MSC)

Field of Science*

  • 1.6 Biological sciences
  • 3.1 Basic medicine
  • 3.4 Medical biotechnology

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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