Chemical genetics reveal the novel transmembrane protein bil4, which mediates plant cell elongation in brassinosteroid signaling

Ayumi Yamagami, Miki Nakazawa, Minami Matsui, Masafumi Tujimoto, Masaaki Sakuta, Tadao Asami, Takeshi Nakano

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Steroid hormones are conserved between animals and plants as signaling molecules to control growth and development. Plant steroid hormones, brassinosteroids (BRs), appear to play an important role in plant cell elongation. BRs bind to leucine-rich repeat kinase BRASSINOSTEROID-INSENSITIVE 1 (BRI1) localized to the plasma membrane, activate transcription factors in collaboration with cytosolic kinases and phosphatases, and regulate BR-responsive gene expression, but the details regarding the BR signaling pathway from perception to nuclear events remain unknown. In this study we used chemical genetics to identify an evolutionarily conserved transmembrane protein, Brz-insensitive-long hypocotyls 4 (BIL4), and demonstrated its role as a critical component of plant cell elongation occurring upon BR signaling. A dominant mutation, bil4-1D, showed cell elongation in the presence of the BR-specific inhibitor Brz. Brz suppresses expression of the BIL4 gene in wild-type plants, and overexpression of BIL4 in bil4-1D suppresses the BR deficiency caused by Brz. Our results indicate that BIL4 mediates cell elongation on BR signaling.

Original languageEnglish
Pages (from-to)415-421
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number2
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Brassinosteroid
  • Brz
  • Cell elongation
  • Seven-transmembrane domain protein
  • Signaling

Field of Science

  • 1.6 Biological sciences
  • 1.4 Chemical sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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