CHEMOKINE RECEPTORS CCR1 AND CCR2 ARE UP-REGULATED ON CIRCULATING LEUKEMIC CELLS OF THE UNTREATED CD38+ CLL PATIENTS WITH THE HIGH EBV DNA-COPY LOAD

Irina Kholodnyuk; Laura Hippe; Alla Rivkina; Simons Svirskis; Alina Sultanova; Svetlana Kozireva; Jelena Pavlova; Modra Murovska; Sandra Lejniece

Abstract

AIM:BACKGROUND AND Chronic lymphocytic leukemia (CLL) is frequently complicated due to immune deregulation and concomitant infections. The high EBV DNA-load had been associated with poor overall survival and disease aggressiveness in CLL patients. Eelier, we demonstrated that CCR1 and CCR2 were up-regulated in peripheral blood (PB) B cells upon in vitro EBV infection.
METHODS:We have measured the EBV DNA-copy numbers in the PB mononuclear cells (PBMC) of 61 newly diagnozed CLL patients, and assessed by polychromatic flow cytometry the cell-surface expression of CCR1, CCR2, and the established negative predictor CD38 in order to estimate correlations.
RESULTS:The positive correlations have been revealed between the presence of CD38 and CCR1 or CCR2 on leukemic cells. Moreover, these three indicators negatively correlated with the frequency of the leukemic cells within the PBMC as well as WBC sets. More than 200 EBV DNA copies per 105 cells were determined in 3 patients only; two of them presented 30% of the CD38+ leukemic cells. We didn’t detect EBNA2 or LMP1 mRNA expression in PBMC of these 3 EBV-positive patients, using real-time RT-PCR. Notably, in the EBV/CD38-positive patients, both CCR1 and CCR2 were highly expressed on the leukemic cells (>20%).
CONCLUSIONS:Migration of the CCR1- and CCR2-expressing leukemic cells from circulation into the secondary lymphoid organs may contribute to aggressive pathogenesis of CLL. The association studies would verify whether the CCR1/CCR2 expression on circulating leukemic cells is a reliable negative predictor in CLL.
SOURCES OF FUNDING:This research was funded by the Latvian Council of Science projects No. 651/2014 and No. lzp-2018/1-0156.

Original language	English
Pages	210
Number of pages	1
Publication status	Published - 15 Sept 2021
Event	23rd Annual Conference of the European Society for Clinical Virology - Manchester, United Kingdom Duration: 15 Sept 2021 → 18 Sept 2021 Conference number: 23 https://www.escv2021.org/conference-information/about-escv/ https://healthmanagement.org/c/hospital/event/23rd-annual-conference-of-the-european-society-for-clinical-virology

Conference

Conference	23rd Annual Conference of the European Society for Clinical Virology
Abbreviated title	ESCV 2021
Country/Territory	United Kingdom
City	Manchester
Period	15/09/21 → 18/09/21
Internet address	https://www.escv2021.org/conference-information/about-escv/ https://healthmanagement.org/c/hospital/event/23rd-annual-conference-of-the-european-society-for-clinical-virology

Keywords*

CLL, EBV, CD38, CCR1, CCR2

Field of Science*

1.6 Biological sciences
3.2 Clinical medicine

Publication Type*

3.4. Other publications in conference proceedings (including local)

Investigation of the chemokine receptors CCR1 and CCR2 and EBV infection aimed on disclosure of new markers that can predict the high risk for progression of chronic lymphocytic leukemia
Holodņuka, I. (Project leader), Kozireva, S. (Participant), Zvejniece, L. (Expert (PhD student)), Pavlova, J. (Participant) & Demida, O. (Assistant (student))
31/08/18 → 30/11/21
Project: Fundamental and Applied Research Programme

Cite this

Kholodnyuk, I., Hippe, L., Rivkina, A., Svirskis, S., Sultanova, A., Kozireva, S., Pavlova, J., Murovska, M., & Lejniece, S. (2021). CHEMOKINE RECEPTORS CCR1 AND CCR2 ARE UP-REGULATED ON CIRCULATING LEUKEMIC CELLS OF THE UNTREATED CD38+ CLL PATIENTS WITH THE HIGH EBV DNA-COPY LOAD. 210. Abstract from 23rd Annual Conference of the European Society for Clinical Virology, Manchester, United Kingdom.

@conference{257677b38a774dfd8bb72d6430fd97bf,

title = "CHEMOKINE RECEPTORS CCR1 AND CCR2 ARE UP-REGULATED ON CIRCULATING LEUKEMIC CELLS OF THE UNTREATED CD38+ CLL PATIENTS WITH THE HIGH EBV DNA-COPY LOAD",

abstract = "AIM:BACKGROUND AND Chronic lymphocytic leukemia (CLL) is frequently complicated due to immune deregulation and concomitant infections. The high EBV DNA-load had been associated with poor overall survival and disease aggressiveness in CLL patients. Eelier, we demonstrated that CCR1 and CCR2 were up-regulated in peripheral blood (PB) B cells upon in vitro EBV infection.METHODS:We have measured the EBV DNA-copy numbers in the PB mononuclear cells (PBMC) of 61 newly diagnozed CLL patients, and assessed by polychromatic flow cytometry the cell-surface expression of CCR1, CCR2, and the established negative predictor CD38 in order to estimate correlations.RESULTS:The positive correlations have been revealed between the presence of CD38 and CCR1 or CCR2 on leukemic cells. Moreover, these three indicators negatively correlated with the frequency of the leukemic cells within the PBMC as well as WBC sets. More than 200 EBV DNA copies per 105 cells were determined in 3 patients only; two of them presented 30% of the CD38+ leukemic cells. We didn{\textquoteright}t detect EBNA2 or LMP1 mRNA expression in PBMC of these 3 EBV-positive patients, using real-time RT-PCR. Notably, in the EBV/CD38-positive patients, both CCR1 and CCR2 were highly expressed on the leukemic cells (>20%).CONCLUSIONS:Migration of the CCR1- and CCR2-expressing leukemic cells from circulation into the secondary lymphoid organs may contribute to aggressive pathogenesis of CLL. The association studies would verify whether the CCR1/CCR2 expression on circulating leukemic cells is a reliable negative predictor in CLL.SOURCES OF FUNDING:This research was funded by the Latvian Council of Science projects No. 651/2014 and No. lzp-2018/1-0156.",

keywords = "CLL, EBV, CD38, CCR1, CCR2",

author = "Irina Kholodnyuk and Laura Hippe and Alla Rivkina and Simons Svirskis and Alina Sultanova and Svetlana Kozireva and Jelena Pavlova and Modra Murovska and Sandra Lejniece",

year = "2021",

month = sep,

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language = "English",

pages = "210",

note = "23rd Annual Conference of the European Society for Clinical Virology, ESCV 2021 ; Conference date: 15-09-2021 Through 18-09-2021",

url = "https://www.escv2021.org/conference-information/about-escv/, https://healthmanagement.org/c/hospital/event/23rd-annual-conference-of-the-european-society-for-clinical-virology",

}

Kholodnyuk, I , Hippe, L, Rivkina, A, Svirskis, S , Sultanova, A , Kozireva, S, Pavlova, J, Murovska, M & Lejniece, S 2021, 'CHEMOKINE RECEPTORS CCR1 AND CCR2 ARE UP-REGULATED ON CIRCULATING LEUKEMIC CELLS OF THE UNTREATED CD38+ CLL PATIENTS WITH THE HIGH EBV DNA-COPY LOAD', 23rd Annual Conference of the European Society for Clinical Virology, Manchester, United Kingdom, 15/09/21 - 18/09/21 pp. 210.

CHEMOKINE RECEPTORS CCR1 AND CCR2 ARE UP-REGULATED ON CIRCULATING LEUKEMIC CELLS OF THE UNTREATED CD38+ CLL PATIENTS WITH THE HIGH EBV DNA-COPY LOAD. / Kholodnyuk, Irina ; Hippe, Laura; Rivkina, Alla et al.
2021. 210 Abstract from 23rd Annual Conference of the European Society for Clinical Virology, Manchester, United Kingdom.

Research output: Contribution to conference › Abstract › peer-review

TY - CONF

T1 - CHEMOKINE RECEPTORS CCR1 AND CCR2 ARE UP-REGULATED ON CIRCULATING LEUKEMIC CELLS OF THE UNTREATED CD38+ CLL PATIENTS WITH THE HIGH EBV DNA-COPY LOAD

AU - Kholodnyuk, Irina

AU - Hippe, Laura

AU - Rivkina, Alla

AU - Svirskis, Simons

AU - Sultanova, Alina

AU - Kozireva, Svetlana

AU - Pavlova, Jelena

AU - Murovska, Modra

AU - Lejniece, Sandra

N1 - Conference code: 23

PY - 2021/9/15

Y1 - 2021/9/15

N2 - AIM:BACKGROUND AND Chronic lymphocytic leukemia (CLL) is frequently complicated due to immune deregulation and concomitant infections. The high EBV DNA-load had been associated with poor overall survival and disease aggressiveness in CLL patients. Eelier, we demonstrated that CCR1 and CCR2 were up-regulated in peripheral blood (PB) B cells upon in vitro EBV infection.METHODS:We have measured the EBV DNA-copy numbers in the PB mononuclear cells (PBMC) of 61 newly diagnozed CLL patients, and assessed by polychromatic flow cytometry the cell-surface expression of CCR1, CCR2, and the established negative predictor CD38 in order to estimate correlations.RESULTS:The positive correlations have been revealed between the presence of CD38 and CCR1 or CCR2 on leukemic cells. Moreover, these three indicators negatively correlated with the frequency of the leukemic cells within the PBMC as well as WBC sets. More than 200 EBV DNA copies per 105 cells were determined in 3 patients only; two of them presented 30% of the CD38+ leukemic cells. We didn’t detect EBNA2 or LMP1 mRNA expression in PBMC of these 3 EBV-positive patients, using real-time RT-PCR. Notably, in the EBV/CD38-positive patients, both CCR1 and CCR2 were highly expressed on the leukemic cells (>20%).CONCLUSIONS:Migration of the CCR1- and CCR2-expressing leukemic cells from circulation into the secondary lymphoid organs may contribute to aggressive pathogenesis of CLL. The association studies would verify whether the CCR1/CCR2 expression on circulating leukemic cells is a reliable negative predictor in CLL.SOURCES OF FUNDING:This research was funded by the Latvian Council of Science projects No. 651/2014 and No. lzp-2018/1-0156.

AB - AIM:BACKGROUND AND Chronic lymphocytic leukemia (CLL) is frequently complicated due to immune deregulation and concomitant infections. The high EBV DNA-load had been associated with poor overall survival and disease aggressiveness in CLL patients. Eelier, we demonstrated that CCR1 and CCR2 were up-regulated in peripheral blood (PB) B cells upon in vitro EBV infection.METHODS:We have measured the EBV DNA-copy numbers in the PB mononuclear cells (PBMC) of 61 newly diagnozed CLL patients, and assessed by polychromatic flow cytometry the cell-surface expression of CCR1, CCR2, and the established negative predictor CD38 in order to estimate correlations.RESULTS:The positive correlations have been revealed between the presence of CD38 and CCR1 or CCR2 on leukemic cells. Moreover, these three indicators negatively correlated with the frequency of the leukemic cells within the PBMC as well as WBC sets. More than 200 EBV DNA copies per 105 cells were determined in 3 patients only; two of them presented 30% of the CD38+ leukemic cells. We didn’t detect EBNA2 or LMP1 mRNA expression in PBMC of these 3 EBV-positive patients, using real-time RT-PCR. Notably, in the EBV/CD38-positive patients, both CCR1 and CCR2 were highly expressed on the leukemic cells (>20%).CONCLUSIONS:Migration of the CCR1- and CCR2-expressing leukemic cells from circulation into the secondary lymphoid organs may contribute to aggressive pathogenesis of CLL. The association studies would verify whether the CCR1/CCR2 expression on circulating leukemic cells is a reliable negative predictor in CLL.SOURCES OF FUNDING:This research was funded by the Latvian Council of Science projects No. 651/2014 and No. lzp-2018/1-0156.

KW - CLL, EBV, CD38, CCR1, CCR2

M3 - Abstract

SP - 210

T2 - 23rd Annual Conference of the European Society for Clinical Virology

Y2 - 15 September 2021 through 18 September 2021

ER -