Chromosomal integration of HHV-6A during non-productive viral infection

Nitish Gulve, Celina Frank, Maximilian Klepsch, Bhupesh K Prusty (Corresponding Author)

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Human herpesvirus 6A (HHV-6A) and 6B (HHV-6B) are two different species of betaherpesviruses that integrate into sub-telomeric ends of human chromosomes, for which different prevalence rates of integration have been reported. It has been demonstrated that integrated viral genome is stable and is fully retained. However, study of chromosomally integrated viral genome in individuals carrying inherited HHV-6 (iciHHV-6) showed unexpected number of viral DR copies. Hence, we created an in vitro infection model and studied retention of full or partial viral genome over a period of time. We observed an exceptional event where cells retained viral direct repeats (DRs) alone in the absence of the full viral genome. Finally, we found evidence for non-telomeric integration of HHV-6A DR in both cultured cells and in an iciHHV-6 individual. Our results shed light on several novel features of HHV-6A chromosomal integration and provide valuable information for future screening techniques.

Original languageEnglish
Pages (from-to)512
Number of pages11
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 30 Mar 2017
Externally publishedYes

Keywords*

  • Base Sequence
  • Cell Line, Tumor
  • Chromosomes, Human/virology
  • Genome, Viral
  • Herpesvirus 6, Human/genetics
  • Humans
  • Repetitive Sequences, Nucleic Acid/genetics
  • Roseolovirus Infections/virology
  • Telomere/genetics
  • Virus Integration/genetics

Field of Science*

  • 3.1 Basic medicine

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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