Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children

Nicola Isabelle Kols, Heli Aatola, Ville Peltola, Man Xu, Zaiga Nora-Krukle, Klaus Hedman, Aurelija Zvirbliene, Hanna Toivola, Tytti Vuorinen, Juha M. Koskinen, Andrea H.L. Bruning, Andreas Christensen, Maria Söderlund-Venermo, Janne O. Koskinen

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Background: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. Objective: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. Study design: HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR. Results and conclusion: We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test.

Original languageEnglish
Pages (from-to)17-19
Number of pages3
JournalJournal of Clinical Virology
Volume120
DOIs
Publication statusPublished - Nov 2019

Keywords

  • Diagnosis
  • Human bocavirus 1
  • Parvovirus
  • Pediatric infection
  • Polymerase chain reaction
  • Rapid antigen detection assay
  • Respiratory tract infection

Field of Science

  • 3.1 Basic medicine
  • 3.2 Clinical medicine

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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