TY - JOUR
T1 - Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children
AU - Kols, Nicola Isabelle
AU - Aatola, Heli
AU - Peltola, Ville
AU - Xu, Man
AU - Nora-Krukle, Zaiga
AU - Hedman, Klaus
AU - Zvirbliene, Aurelija
AU - Toivola, Hanna
AU - Vuorinen, Tytti
AU - Koskinen, Juha M.
AU - Bruning, Andrea H.L.
AU - Christensen, Andreas
AU - Söderlund-Venermo, Maria
AU - Koskinen, Janne O.
N1 - Funding Information:
This work was supported by the Sigrid Jusélius Foundation , Finland [no grant number]; the Life and Health Medical Grant Association , Finland [no grant number]; the China Scholarship Council , China [ 201406170014 ]; the Jane and Aatos Erkko Foundation , Finland [no grant number]; the Finnish Funding Agency for Innovation , Finland, under the project name ‘Get it done!’ [funding decision 534/14]; and BALTINFECT, within the European Union 7 th framework programme [project no. 316275].
Publisher Copyright:
© 2019 Elsevier B.V.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/11
Y1 - 2019/11
N2 - Background: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. Objective: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. Study design: HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR. Results and conclusion: We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test.
AB - Background: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. Objective: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. Study design: HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR. Results and conclusion: We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test.
KW - Diagnosis
KW - Human bocavirus 1
KW - Parvovirus
KW - Pediatric infection
KW - Polymerase chain reaction
KW - Rapid antigen detection assay
KW - Respiratory tract infection
UR - http://www.scopus.com/inward/record.url?scp=85071978107&partnerID=8YFLogxK
U2 - 10.1016/j.jcv.2019.09.003
DO - 10.1016/j.jcv.2019.09.003
M3 - Article
C2 - 31521013
AN - SCOPUS:85071978107
SN - 1386-6532
VL - 120
SP - 17
EP - 19
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
ER -
