Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children

Nicola Isabelle Kols; Heli Aatola; Ville Peltola; Man Xu; Zaiga Nora-Krukle; Klaus Hedman; Aurelija Zvirbliene; Hanna Toivola; Tytti Vuorinen; Juha M. Koskinen; Andrea H.L. Bruning; Andreas Christensen; Maria Söderlund-Venermo; Janne O. Koskinen

doi:10.1016/j.jcv.2019.09.003

Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children

Nicola Isabelle Kols
, Heli Aatola
, Ville Peltola
, Man Xu
, Zaiga Nora-Krukle
, Klaus Hedman
, Aurelija Zvirbliene
, Hanna Toivola
, Tytti Vuorinen
, Juha M. Koskinen
, Andrea H.L. Bruning
, Andreas Christensen
, Maria Söderlund-Venermo
, Janne O. Koskinen

Institute of Microbiology and Virology

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Background: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. Objective: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. Study design: HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR. Results and conclusion: We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test.

Original language	English
Pages (from-to)	17-19
Number of pages	3
Journal	Journal of Clinical Virology
Volume	120
DOIs	https://doi.org/10.1016/j.jcv.2019.09.003
Publication status	Published - Nov 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords*

Diagnosis
Human bocavirus 1
Parvovirus
Pediatric infection
Polymerase chain reaction
Rapid antigen detection assay
Respiratory tract infection

Field of Science*

3.2 Clinical medicine
3.1 Basic medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1016/j.jcv.2019.09.003

Cite this

Kols, N. I., Aatola, H., Peltola, V., Xu, M., Nora-Krukle, Z., Hedman, K., Zvirbliene, A., Toivola, H., Vuorinen, T., Koskinen, J. M., Bruning, A. H. L., Christensen, A., Söderlund-Venermo, M., & Koskinen, J. O. (2019). Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children. Journal of Clinical Virology, 120, 17-19. https://doi.org/10.1016/j.jcv.2019.09.003

@article{de176ffc5ce84f65ba85c7533039b35a,

title = "Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children",

abstract = "Background: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. Objective: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. Study design: HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR. Results and conclusion: We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test.",

keywords = "Diagnosis, Human bocavirus 1, Parvovirus, Pediatric infection, Polymerase chain reaction, Rapid antigen detection assay, Respiratory tract infection",

author = "Kols, \{Nicola Isabelle\} and Heli Aatola and Ville Peltola and Man Xu and Zaiga Nora-Krukle and Klaus Hedman and Aurelija Zvirbliene and Hanna Toivola and Tytti Vuorinen and Koskinen, \{Juha M.\} and Bruning, \{Andrea H.L.\} and Andreas Christensen and Maria S{\"o}derlund-Venermo and Koskinen, \{Janne O.\}",

note = "Funding Information: This work was supported by the Sigrid Jus{\'e}lius Foundation , Finland [no grant number]; the Life and Health Medical Grant Association , Finland [no grant number]; the China Scholarship Council , China [ 201406170014 ]; the Jane and Aatos Erkko Foundation , Finland [no grant number]; the Finnish Funding Agency for Innovation , Finland, under the project name {\textquoteleft}Get it done!{\textquoteright} [funding decision 534/14]; and BALTINFECT, within the European Union 7 th framework programme [project no. 316275]. Publisher Copyright: {\textcopyright} 2019 Elsevier B.V. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",

year = "2019",

month = nov,

doi = "10.1016/j.jcv.2019.09.003",

language = "English",

volume = "120",

pages = "17--19",

journal = "Journal of Clinical Virology",

issn = "1386-6532",

publisher = "Elsevier B.V.",

}

Kols, NI, Aatola, H, Peltola, V, Xu, M, Nora-Krukle, Z, Hedman, K, Zvirbliene, A, Toivola, H, Vuorinen, T, Koskinen, JM, Bruning, AHL, Christensen, A, Söderlund-Venermo, M & Koskinen, JO 2019, 'Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children', Journal of Clinical Virology, vol. 120, pp. 17-19. https://doi.org/10.1016/j.jcv.2019.09.003

TY - JOUR

T1 - Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children

AU - Kols, Nicola Isabelle

AU - Aatola, Heli

AU - Peltola, Ville

AU - Xu, Man

AU - Nora-Krukle, Zaiga

AU - Hedman, Klaus

AU - Zvirbliene, Aurelija

AU - Toivola, Hanna

AU - Vuorinen, Tytti

AU - Koskinen, Juha M.

AU - Bruning, Andrea H.L.

AU - Christensen, Andreas

AU - Söderlund-Venermo, Maria

AU - Koskinen, Janne O.

N1 - Funding Information: This work was supported by the Sigrid Jusélius Foundation , Finland [no grant number]; the Life and Health Medical Grant Association , Finland [no grant number]; the China Scholarship Council , China [ 201406170014 ]; the Jane and Aatos Erkko Foundation , Finland [no grant number]; the Finnish Funding Agency for Innovation , Finland, under the project name ‘Get it done!’ [funding decision 534/14]; and BALTINFECT, within the European Union 7 th framework programme [project no. 316275]. Publisher Copyright: © 2019 Elsevier B.V. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2019/11

Y1 - 2019/11

N2 - Background: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. Objective: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. Study design: HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR. Results and conclusion: We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test.

AB - Background: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. Objective: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. Study design: HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR. Results and conclusion: We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test.

KW - Diagnosis

KW - Human bocavirus 1

KW - Parvovirus

KW - Pediatric infection

KW - Polymerase chain reaction

KW - Rapid antigen detection assay

KW - Respiratory tract infection

UR - https://www.scopus.com/pages/publications/85071978107

U2 - 10.1016/j.jcv.2019.09.003

DO - 10.1016/j.jcv.2019.09.003

M3 - Article

C2 - 31521013

AN - SCOPUS:85071978107

SN - 1386-6532

VL - 120

SP - 17

EP - 19

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

ER -

Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children

Abstract

UN SDGs

Keywords*

Field of Science*

Publication Type*

Access to Document

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