Introduction: Clinical outcomes of Helicobacter pylori (Hp) infection are associated both with host and bacterial virulence factors. It has been shown that presence of cytotoxin-associated gene (cagA), responsible for CagA protein
production, is associated with more severe clinical outcome such as atrophic
gastritis and adenocarcinoma. Aim of the study was to find out if there is interrater agreement between two methods for detection of Hp anti-CagA antibodies: enzyme-linked immunosorbent assay (ELISA) and immunoblot.
Materials and Methods: Sera from 303 patients (median of age 61 years,
males/females 112/191) with dyspeptic symptoms coming for diagnostic gastroscopy were collected. IgG immunoblot (Mikrogen Diagnostik, Germany) and
total IgM, IgA and IgG ELISA (Vector-BEST, Russia) were used to detect Hp anti-CagA antibodies according to the manufacturer‘s instructions. Inter-rater
agreement was calculated as kappa (k) value.
Results: A significant inter-rater agreement between both test systems
(j = 0.592, p = 0.043) for anti-CagA detection was observed. As shown in table
immunoblot system identified extra 59 positive cases. Conclusions: Both test system has good inter-rater agreement, but immunoblot maybe considered as more sensitive test system for detection of CagA status. Further research comparing the data for Hp infection status and clinical
outcome is necessary. Questionable remains time period in which anti-CagA
disappear after eradication of HP.
Acknowledgements: Study was supported by ERDF project Nr.2010/0302/
- 3.1 Basic medicine
- 3.2 Clinical medicine
- 3.3. Publications in conference proceedings indexed in Web of Science and/or Scopus database