COMPARISON OF TWO SEROLOGICAL TESTS TO DETERMINE THE CAGA STATUS IN DYSPEPTIC LATVIAN PATIENTS

Dace Rudzite; Ilva Daugule; Georgijs Moisejevs; Markus Gerhard; Gereon Göttner; Thorsten Zacher; Sergey Krotov; Irina Davidjuka; Dainius Janciauskas; Inta Liepniece-Karele; Sergejs Isajevs; Ieva Lasina; Konrads Funka; Ilze Kikuste; Aigars Vanags; Ivars Tolmanis; Marcis Leja

Abstract

Introduction: Clinical outcomes of Helicobacter pylori (Hp) infection are associated both with host and bacterial virulence factors. It has been shown that presence of cytotoxin-associated gene (cagA), responsible for CagA protein production, is associated with more severe clinical outcome such as atrophic gastritis and adenocarcinoma. Aim of the study was to find out if there is interrater agreement between two methods for detection of Hp anti-CagA antibodies: enzyme-linked immunosorbent assay (ELISA) and immunoblot. Materials and Methods: Sera from 303 patients (median of age 61 years, males/females 112/191) with dyspeptic symptoms coming for diagnostic gastroscopy were collected. IgG immunoblot (Mikrogen Diagnostik, Germany) and total IgM, IgA and IgG ELISA (Vector-BEST, Russia) were used to detect Hp anti-CagA antibodies according to the manufacturer‘s instructions. Inter-rater agreement was calculated as kappa (k) value. Results: A significant inter-rater agreement between both test systems (j = 0.592, p = 0.043) for anti-CagA detection was observed. As shown in table immunoblot system identified extra 59 positive cases. Conclusions: Both test system has good inter-rater agreement, but immunoblot maybe considered as more sensitive test system for detection of CagA status. Further research comparing the data for Hp infection status and clinical outcome is necessary. Questionable remains time period in which anti-CagA disappear after eradication of HP. Acknowledgements: Study was supported by ERDF project Nr.2010/0302/ 2DP/2.1.1.1.0/10/APIA/VIAA/158.

Original language	English
Article number	P10.16
Pages (from-to)	122-123
Journal	Helicobacter
Volume	18
Issue number	Suppl.1
Publication status	Published - Sept 2013
Event	XXVIth International Workshop on Helicobacter and Related Bacteria in Chronic Digestive Inflammation and Gastric Cancer - Madrid, Spain Duration: 12 Sept 2013 → 14 Sept 2013 Conference number: 26 https://www.nutricion.ucr.ac.cr/index.php/es/proyectos/publicaciones/376-geographic-origin-of-helicobacter-pylori-isolated-from-costa-rican-patients-european-helicobacter-study-group-xxvith-international-workshop-on-helicobacter-and-related-bacteria-in-chronic-digestive-inflammation-and-gastric-cancer

Field of Science*

3.1 Basic medicine
3.2 Clinical medicine

Publication Type*

3.3. Publications in conference proceedings indexed in Web of Science and/or Scopus database

Access to Document

https://doi.org/10.1111/hel.12079

Cite this

Rudzite, D., Daugule, I., Moisejevs, G., Gerhard, M., Göttner, G., Zacher, T., Krotov, S., Davidjuka, I., Janciauskas, D., Liepniece-Karele, I., Isajevs, S., Lasina, I., Funka, K., Kikuste, I., Vanags, A., Tolmanis, I., & Leja, M. (2013). COMPARISON OF TWO SEROLOGICAL TESTS TO DETERMINE THE CAGA STATUS IN DYSPEPTIC LATVIAN PATIENTS. Helicobacter, 18(Suppl.1), 122-123. Article P10.16. https://doi.org/10.1111/hel.12079

@article{4ce472c313cd4134b2540093e9366031,

title = "COMPARISON OF TWO SEROLOGICAL TESTS TO DETERMINE THE CAGA STATUS IN DYSPEPTIC LATVIAN PATIENTS",

abstract = "Introduction: Clinical outcomes of Helicobacter pylori (Hp) infection are associated both with host and bacterial virulence factors. It has been shown that presence of cytotoxin-associated gene (cagA), responsible for CagA protein production, is associated with more severe clinical outcome such as atrophic gastritis and adenocarcinoma. Aim of the study was to find out if there is interrater agreement between two methods for detection of Hp anti-CagA antibodies: enzyme-linked immunosorbent assay (ELISA) and immunoblot. Materials and Methods: Sera from 303 patients (median of age 61 years, males/females 112/191) with dyspeptic symptoms coming for diagnostic gastroscopy were collected. IgG immunoblot (Mikrogen Diagnostik, Germany) and total IgM, IgA and IgG ELISA (Vector-BEST, Russia) were used to detect Hp anti-CagA antibodies according to the manufacturer{\textquoteleft}s instructions. Inter-rater agreement was calculated as kappa (k) value. Results: A significant inter-rater agreement between both test systems (j = 0.592, p = 0.043) for anti-CagA detection was observed. As shown in table immunoblot system identified extra 59 positive cases. Conclusions: Both test system has good inter-rater agreement, but immunoblot maybe considered as more sensitive test system for detection of CagA status. Further research comparing the data for Hp infection status and clinical outcome is necessary. Questionable remains time period in which anti-CagA disappear after eradication of HP. Acknowledgements: Study was supported by ERDF project Nr.2010/0302/ 2DP/2.1.1.1.0/10/APIA/VIAA/158.",

author = "Dace Rudzite and Ilva Daugule and Georgijs Moisejevs and Markus Gerhard and Gereon G{\"o}ttner and Thorsten Zacher and Sergey Krotov and Irina Davidjuka and Dainius Janciauskas and Inta Liepniece-Karele and Sergejs Isajevs and Ieva Lasina and Konrads Funka and Ilze Kikuste and Aigars Vanags and Ivars Tolmanis and Marcis Leja",

year = "2013",

month = sep,

language = "English",

volume = "18",

pages = "122--123",

journal = "Helicobacter",

issn = "1083-4389",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "Suppl.1",

note = "XXVIth International Workshop on Helicobacter and Related Bacteria in Chronic Digestive Inflammation and Gastric Cancer ; Conference date: 12-09-2013 Through 14-09-2013",

url = "https://www.nutricion.ucr.ac.cr/index.php/es/proyectos/publicaciones/376-geographic-origin-of-helicobacter-pylori-isolated-from-costa-rican-patients-european-helicobacter-study-group-xxvith-international-workshop-on-helicobacter-and-related-bacteria-in-chronic-digestive-inflammation-and-gastric-cancer",

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Rudzite, D, Daugule, I, Moisejevs, G, Gerhard, M, Göttner, G, Zacher, T, Krotov, S, Davidjuka, I, Janciauskas, D, Liepniece-Karele, I, Isajevs, S, Lasina, I, Funka, K, Kikuste, I, Vanags, A, Tolmanis, I & Leja, M 2013, 'COMPARISON OF TWO SEROLOGICAL TESTS TO DETERMINE THE CAGA STATUS IN DYSPEPTIC LATVIAN PATIENTS', Helicobacter, vol. 18, no. Suppl.1, P10.16, pp. 122-123. < https://doi.org/10.1111/hel.12079>

TY - JOUR

T1 - COMPARISON OF TWO SEROLOGICAL TESTS TO DETERMINE THE CAGA STATUS IN DYSPEPTIC LATVIAN PATIENTS

AU - Rudzite, Dace

AU - Daugule, Ilva

AU - Moisejevs, Georgijs

AU - Gerhard, Markus

AU - Göttner, Gereon

AU - Zacher, Thorsten

AU - Krotov, Sergey

AU - Davidjuka, Irina

AU - Janciauskas, Dainius

AU - Liepniece-Karele, Inta

AU - Isajevs, Sergejs

AU - Lasina, Ieva

AU - Funka, Konrads

AU - Kikuste, Ilze

AU - Vanags, Aigars

AU - Tolmanis, Ivars

AU - Leja, Marcis

N1 - Conference code: 26

PY - 2013/9

Y1 - 2013/9

N2 - Introduction: Clinical outcomes of Helicobacter pylori (Hp) infection are associated both with host and bacterial virulence factors. It has been shown that presence of cytotoxin-associated gene (cagA), responsible for CagA protein production, is associated with more severe clinical outcome such as atrophic gastritis and adenocarcinoma. Aim of the study was to find out if there is interrater agreement between two methods for detection of Hp anti-CagA antibodies: enzyme-linked immunosorbent assay (ELISA) and immunoblot. Materials and Methods: Sera from 303 patients (median of age 61 years, males/females 112/191) with dyspeptic symptoms coming for diagnostic gastroscopy were collected. IgG immunoblot (Mikrogen Diagnostik, Germany) and total IgM, IgA and IgG ELISA (Vector-BEST, Russia) were used to detect Hp anti-CagA antibodies according to the manufacturer‘s instructions. Inter-rater agreement was calculated as kappa (k) value. Results: A significant inter-rater agreement between both test systems (j = 0.592, p = 0.043) for anti-CagA detection was observed. As shown in table immunoblot system identified extra 59 positive cases. Conclusions: Both test system has good inter-rater agreement, but immunoblot maybe considered as more sensitive test system for detection of CagA status. Further research comparing the data for Hp infection status and clinical outcome is necessary. Questionable remains time period in which anti-CagA disappear after eradication of HP. Acknowledgements: Study was supported by ERDF project Nr.2010/0302/ 2DP/2.1.1.1.0/10/APIA/VIAA/158.

AB - Introduction: Clinical outcomes of Helicobacter pylori (Hp) infection are associated both with host and bacterial virulence factors. It has been shown that presence of cytotoxin-associated gene (cagA), responsible for CagA protein production, is associated with more severe clinical outcome such as atrophic gastritis and adenocarcinoma. Aim of the study was to find out if there is interrater agreement between two methods for detection of Hp anti-CagA antibodies: enzyme-linked immunosorbent assay (ELISA) and immunoblot. Materials and Methods: Sera from 303 patients (median of age 61 years, males/females 112/191) with dyspeptic symptoms coming for diagnostic gastroscopy were collected. IgG immunoblot (Mikrogen Diagnostik, Germany) and total IgM, IgA and IgG ELISA (Vector-BEST, Russia) were used to detect Hp anti-CagA antibodies according to the manufacturer‘s instructions. Inter-rater agreement was calculated as kappa (k) value. Results: A significant inter-rater agreement between both test systems (j = 0.592, p = 0.043) for anti-CagA detection was observed. As shown in table immunoblot system identified extra 59 positive cases. Conclusions: Both test system has good inter-rater agreement, but immunoblot maybe considered as more sensitive test system for detection of CagA status. Further research comparing the data for Hp infection status and clinical outcome is necessary. Questionable remains time period in which anti-CagA disappear after eradication of HP. Acknowledgements: Study was supported by ERDF project Nr.2010/0302/ 2DP/2.1.1.1.0/10/APIA/VIAA/158.

M3 - Meeting Abstract

SN - 1083-4389

VL - 18

SP - 122

EP - 123

JO - Helicobacter

JF - Helicobacter

IS - Suppl.1

M1 - P10.16

T2 - XXVIth International Workshop on Helicobacter and Related Bacteria in Chronic Digestive Inflammation and Gastric Cancer

Y2 - 12 September 2013 through 14 September 2013