COMPARISON OF TWO SEROLOGICAL TESTS TO DETERMINE THE CAGA STATUS IN DYSPEPTIC LATVIAN PATIENTS

Dace Rudzite, Ilva Daugule, Georgijs Moisejevs, Markus Gerhard, Gereon Göttner, Thorsten Zacher, Sergey Krotov, Irina Davidjuka, Dainius Janciauskas, Inta Liepniece-Karele, Sergejs Isajevs, Ieva Lasina, Konrads Funka, Ilze Kikuste, Aigars Vanags, Ivars Tolmanis, Marcis Leja

Research output: Contribution to journalMeeting Abstractpeer-review

Abstract

Introduction: Clinical outcomes of Helicobacter pylori (Hp) infection are associated both with host and bacterial virulence factors. It has been shown that presence of cytotoxin-associated gene (cagA), responsible for CagA protein production, is associated with more severe clinical outcome such as atrophic gastritis and adenocarcinoma. Aim of the study was to find out if there is interrater agreement between two methods for detection of Hp anti-CagA antibodies: enzyme-linked immunosorbent assay (ELISA) and immunoblot. Materials and Methods: Sera from 303 patients (median of age 61 years, males/females 112/191) with dyspeptic symptoms coming for diagnostic gastroscopy were collected. IgG immunoblot (Mikrogen Diagnostik, Germany) and total IgM, IgA and IgG ELISA (Vector-BEST, Russia) were used to detect Hp anti-CagA antibodies according to the manufacturer‘s instructions. Inter-rater agreement was calculated as kappa (k) value. Results: A significant inter-rater agreement between both test systems (j = 0.592, p = 0.043) for anti-CagA detection was observed. As shown in table immunoblot system identified extra 59 positive cases.  Conclusions: Both test system has good inter-rater agreement, but immunoblot maybe considered as more sensitive test system for detection of CagA status. Further research comparing the data for Hp infection status and clinical outcome is necessary. Questionable remains time period in which anti-CagA disappear after eradication of HP. Acknowledgements: Study was supported by ERDF project Nr.2010/0302/ 2DP/2.1.1.1.0/10/APIA/VIAA/158.
Original languageEnglish
Article numberP10.16
Pages (from-to)122-123
JournalHelicobacter
Volume18
Issue numberSuppl.1
Publication statusPublished - Sep 2013
EventXXVIth International Workshop on Helicobacter and
Related Bacteria in Chronic Digestive Inflammation and Gastric Cancer
- Madrid, Spain
Duration: 12 Sep 201314 Sep 2013

Field of Science*

  • 3.1 Basic medicine
  • 3.2 Clinical medicine

Publication Type*

  • 3.3. Publications in conference proceedings indexed in Web of Science and/or Scopus database

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