Consistent downregulation of human lactoferrin gene, in the common eliminated region 1 on 3p21.3, following tumor growth in severe combined immunodeficient (SCID) mice

Ying Yang; Jingfeng Li; Anna Szeles; Marta P. Imreh; Maria Kost-Alimova; Hajnalka Kiss; Irina Kholodnyuk; Ludmilla Fedorova; Eva Darai; George Klein; Stefan Imreh

doi:10.1016/S0304-3835(02)00677-8

Consistent downregulation of human lactoferrin gene, in the common eliminated region 1 on 3p21.3, following tumor growth in severe combined immunodeficient (SCID) mice

Ying Yang, Jingfeng Li, Anna Szeles, Marta P. Imreh, Maria Kost-Alimova, Hajnalka Kiss, Irina Kholodnyuk, Ludmilla Fedorova, Eva Darai, George Klein, Stefan Imreh

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Lactoferrin (LF) is one of 19 active genes in the common eliminated region 1 at 3p21.3 identified by us. LF was transfected into mouse fibrosarcoma A9. Fourteen severe combined immunodeficient (SCID) derived tumors from two PI based artificial chromosome (PAC)-transfectants containing the entire LF gene and two LF-cDNA transfectants were analyzed by real time polymerase chain reaction at the DNA and RNA level. Following SCID tumor passage, LF expression was decreased or eclipsed, in all tumors although DNA levels did not change considerably. Promoter methylation and/or rearrangement of the insertion site may be responsible for human LF downregulation in mouse fibrosarcoma derived tumors.

Original language	English
Pages (from-to)	155-164
Number of pages	10
Journal	Cancer Letters
Volume	191
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3835(02)00677-8
Publication status	Published - 10 Mar 2003
Externally published	Yes

Keywords*

3p21.3
Common eliminated region 1 (CER1)
Elimination test
Human lactoferrin gene
Real time-polymerase chain reaction

Field of Science*

3.2 Clinical medicine
1.6 Biological sciences

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S0304-3835(02)00677-8

Cite this

Yang, Y., Li, J., Szeles, A., Imreh, M. P., Kost-Alimova, M., Kiss, H., Kholodnyuk, I., Fedorova, L., Darai, E., Klein, G., & Imreh, S. (2003). Consistent downregulation of human lactoferrin gene, in the common eliminated region 1 on 3p21.3, following tumor growth in severe combined immunodeficient (SCID) mice. Cancer Letters, 191(2), 155-164. https://doi.org/10.1016/S0304-3835(02)00677-8

@article{dd940236ec1a48299c8b8691ba0f193f,

title = "Consistent downregulation of human lactoferrin gene, in the common eliminated region 1 on 3p21.3, following tumor growth in severe combined immunodeficient (SCID) mice",

abstract = "Lactoferrin (LF) is one of 19 active genes in the common eliminated region 1 at 3p21.3 identified by us. LF was transfected into mouse fibrosarcoma A9. Fourteen severe combined immunodeficient (SCID) derived tumors from two PI based artificial chromosome (PAC)-transfectants containing the entire LF gene and two LF-cDNA transfectants were analyzed by real time polymerase chain reaction at the DNA and RNA level. Following SCID tumor passage, LF expression was decreased or eclipsed, in all tumors although DNA levels did not change considerably. Promoter methylation and/or rearrangement of the insertion site may be responsible for human LF downregulation in mouse fibrosarcoma derived tumors.",

keywords = "3p21.3, Common eliminated region 1 (CER1), Elimination test, Human lactoferrin gene, Real time-polymerase chain reaction",

author = "Ying Yang and Jingfeng Li and Anna Szeles and Imreh, {Marta P.} and Maria Kost-Alimova and Hajnalka Kiss and Irina Kholodnyuk and Ludmilla Fedorova and Eva Darai and George Klein and Stefan Imreh",

note = "Funding Information: Human lactoferrin cDNA was kindly provided by Dr Philip Furmanski NY University. This work was supported by: the Swedish Cancer Society and by the Cancer Research Institute/Concern Foundation, NY, LA. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2003",

month = mar,

day = "10",

doi = "10.1016/S0304-3835(02)00677-8",

language = "English",

volume = "191",

pages = "155--164",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

Yang, Y, Li, J, Szeles, A, Imreh, MP, Kost-Alimova, M, Kiss, H, Kholodnyuk, I, Fedorova, L, Darai, E, Klein, G & Imreh, S 2003, 'Consistent downregulation of human lactoferrin gene, in the common eliminated region 1 on 3p21.3, following tumor growth in severe combined immunodeficient (SCID) mice', Cancer Letters, vol. 191, no. 2, pp. 155-164. https://doi.org/10.1016/S0304-3835(02)00677-8

TY - JOUR

T1 - Consistent downregulation of human lactoferrin gene, in the common eliminated region 1 on 3p21.3, following tumor growth in severe combined immunodeficient (SCID) mice

AU - Yang, Ying

AU - Li, Jingfeng

AU - Szeles, Anna

AU - Imreh, Marta P.

AU - Kost-Alimova, Maria

AU - Kiss, Hajnalka

AU - Kholodnyuk, Irina

AU - Fedorova, Ludmilla

AU - Darai, Eva

AU - Klein, George

AU - Imreh, Stefan

N1 - Funding Information: Human lactoferrin cDNA was kindly provided by Dr Philip Furmanski NY University. This work was supported by: the Swedish Cancer Society and by the Cancer Research Institute/Concern Foundation, NY, LA. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2003/3/10

Y1 - 2003/3/10

N2 - Lactoferrin (LF) is one of 19 active genes in the common eliminated region 1 at 3p21.3 identified by us. LF was transfected into mouse fibrosarcoma A9. Fourteen severe combined immunodeficient (SCID) derived tumors from two PI based artificial chromosome (PAC)-transfectants containing the entire LF gene and two LF-cDNA transfectants were analyzed by real time polymerase chain reaction at the DNA and RNA level. Following SCID tumor passage, LF expression was decreased or eclipsed, in all tumors although DNA levels did not change considerably. Promoter methylation and/or rearrangement of the insertion site may be responsible for human LF downregulation in mouse fibrosarcoma derived tumors.

AB - Lactoferrin (LF) is one of 19 active genes in the common eliminated region 1 at 3p21.3 identified by us. LF was transfected into mouse fibrosarcoma A9. Fourteen severe combined immunodeficient (SCID) derived tumors from two PI based artificial chromosome (PAC)-transfectants containing the entire LF gene and two LF-cDNA transfectants were analyzed by real time polymerase chain reaction at the DNA and RNA level. Following SCID tumor passage, LF expression was decreased or eclipsed, in all tumors although DNA levels did not change considerably. Promoter methylation and/or rearrangement of the insertion site may be responsible for human LF downregulation in mouse fibrosarcoma derived tumors.

KW - 3p21.3

KW - Common eliminated region 1 (CER1)

KW - Elimination test

KW - Human lactoferrin gene

KW - Real time-polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0037430552&partnerID=8YFLogxK

U2 - 10.1016/S0304-3835(02)00677-8

DO - 10.1016/S0304-3835(02)00677-8

M3 - Article

C2 - 12618328

AN - SCOPUS:0037430552

SN - 0304-3835

VL - 191

SP - 155

EP - 164

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Consistent downregulation of human lactoferrin gene, in the common eliminated region 1 on 3p21.3, following tumor growth in severe combined immunodeficient (SCID) mice

Abstract

Keywords*

Field of Science*

Publication Type*

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this