Consistent downregulation of human lactoferrin gene, in the common eliminated region 1 on 3p21.3, following tumor growth in severe combined immunodeficient (SCID) mice

Ying Yang, Jingfeng Li, Anna Szeles, Marta P. Imreh, Maria Kost-Alimova, Hajnalka Kiss, Irina Kholodnyuk, Ludmilla Fedorova, Eva Darai, George Klein, Stefan Imreh

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Lactoferrin (LF) is one of 19 active genes in the common eliminated region 1 at 3p21.3 identified by us. LF was transfected into mouse fibrosarcoma A9. Fourteen severe combined immunodeficient (SCID) derived tumors from two PI based artificial chromosome (PAC)-transfectants containing the entire LF gene and two LF-cDNA transfectants were analyzed by real time polymerase chain reaction at the DNA and RNA level. Following SCID tumor passage, LF expression was decreased or eclipsed, in all tumors although DNA levels did not change considerably. Promoter methylation and/or rearrangement of the insertion site may be responsible for human LF downregulation in mouse fibrosarcoma derived tumors.

Original languageEnglish
Pages (from-to)155-164
Number of pages10
JournalCancer Letters
Volume191
Issue number2
DOIs
Publication statusPublished - 10 Mar 2003
Externally publishedYes

Keywords

  • 3p21.3
  • Common eliminated region 1 (CER1)
  • Elimination test
  • Human lactoferrin gene
  • Real time-polymerase chain reaction

Field of Science

  • 3.2 Clinical medicine
  • 1.6 Biological sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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