Construction of Representative NotI Linking Libraries Specific for the Total Human Genome and for Human Chromosome 3

Eugene R. Zabarovsky, Rando Allikmets, Irina Kholodnyuk, Veronika I. Zabarovska, Niklas Paulsson, Vladimir M. Bannikov, Vladimir I. Kashuba, Michael Dean, Lev L. Kisselev, George Klein

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

NotI linking clones represent valuable tools for both physical and genetic mapping. Using procedures that we have previously described, several chromosome 3-specific NotI linking libraries have been constructed. Here, we describe the construction of six independent NotI linking libraries specific for the total human genome. These libraries were made using three different vectors and two combinations of restriction enzymes. Altogether, these six libraries contain more than 1 million recombinant phages. Considering that the human genome contains about 3000-5000 NotI sites, it is likely that all clonable NotI sites are present in these libraries. Two of the six libraries were transferred into plasmid form. At the same time, a chromosome 3-specific EcoRI-NotI library (NRL1) was constructed. This library considerably increases the representation of cloned NotI sites in combination with previously constructed libraries that were made using Bam HI-NotI digestion. All libraries are available on request.

Original languageEnglish
Pages (from-to)312-316
Number of pages5
JournalGenomics
Volume20
Issue number2
DOIs
Publication statusPublished - 15 Mar 1994
Externally publishedYes

Field of Science

  • 1.6 Biological sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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