Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids

Elizaveta V Koudan; Alla I Zorina; Aleksandr A Levin; Frederico D A S Pereira; Stanislav V Petrov; Saida Sh Karshieva; Vladimir A Kasyanov; Natalya E Manturova; Andrey Yu Ustyugov; Nikolay N Potekaev; Vladislav A Parfenov; Pavel A Karalkin; Yusef D Khesuani; Elena A Bulanova; Pavel B Kopnin; Artur A Isaev; Vladimir A Mironov; Vadim L Zorin

doi:10.1007/s00441-022-03690-1

Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids

Elizaveta V Koudan (Corresponding Author), Alla I Zorina, Aleksandr A Levin, Frederico D A S Pereira, Stanislav V Petrov, Saida Sh Karshieva, Vladimir A Kasyanov, Natalya E Manturova, Andrey Yu Ustyugov, Nikolay N Potekaev, Vladislav A Parfenov, Pavel A Karalkin, Yusef D Khesuani, Elena A Bulanova, Pavel B Kopnin, Artur A Isaev, Vladimir A Mironov, Vadim L Zorin

Rīga Stradiņš University

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

In situ 3D bioprinting is a new emerging therapeutic modality for treating human skin diseases. The tissue spheroids have been previously suggested as a powerful tool in rapidly expanding bioprinting technology. It has been demonstrated that the regenerative potential of human dermal fibroblasts could be quantitatively evaluated in 2D cell culture and confirmed after implantation in vivo. However, the development of unbiassed quantitative criteria of the regenerative potential of 3D tissue spheroids in vitro before their in situ bioprinting remains to be investigated. Here it has been demonstrated for the first time that specific correlations exist between the regenerative potential of human dermal fibroblasts cultured in vitro as 2D cell monolayer with biological properties of 3D tissue spheroids fabricated from these fibroblasts. In vitro assessment of biological properties included diameter, spreading and fusion kinetics, and biomechanical properties of 3D tissue spheroids. This comprehensive characterization could be used to predict tissue spheroids' regenerative potential in vivo.

Original language	English
Pages (from-to)	453-464
Number of pages	12
Journal	Cell and Tissue Research
Volume	390
Issue number	3
DOIs	https://doi.org/10.1007/s00441-022-03690-1
Publication status	Published - Dec 2022

Keywords*

Clonogenicity
Dermal fibroblasts
Proliferation
Regenerative potential
Tissue spheroids

Field of Science*

3.2 Clinical medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/s00441-022-03690-1

Cite this

Koudan, E. V., Zorina, A. I., Levin, A. A., Pereira, F. D. A. S., Petrov, S. V., Karshieva, S. S., Kasyanov, V. A., Manturova, N. E., Ustyugov, A. Y., Potekaev, N. N., Parfenov, V. A., Karalkin, P. A., Khesuani, Y. D., Bulanova, E. A., Kopnin, P. B., Isaev, A. A., Mironov, V. A., & Zorin, V. L. (2022). Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids. Cell and Tissue Research, 390(3), 453-464. https://doi.org/10.1007/s00441-022-03690-1

@article{22670c2ab0184c9e9f4a35ecdbf06eff,

title = "Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids",

abstract = "In situ 3D bioprinting is a new emerging therapeutic modality for treating human skin diseases. The tissue spheroids have been previously suggested as a powerful tool in rapidly expanding bioprinting technology. It has been demonstrated that the regenerative potential of human dermal fibroblasts could be quantitatively evaluated in 2D cell culture and confirmed after implantation in vivo. However, the development of unbiassed quantitative criteria of the regenerative potential of 3D tissue spheroids in vitro before their in situ bioprinting remains to be investigated. Here it has been demonstrated for the first time that specific correlations exist between the regenerative potential of human dermal fibroblasts cultured in vitro as 2D cell monolayer with biological properties of 3D tissue spheroids fabricated from these fibroblasts. In vitro assessment of biological properties included diameter, spreading and fusion kinetics, and biomechanical properties of 3D tissue spheroids. This comprehensive characterization could be used to predict tissue spheroids' regenerative potential in vivo.",

keywords = "Clonogenicity, Dermal fibroblasts, Proliferation, Regenerative potential, Tissue spheroids",

author = "Koudan, {Elizaveta V} and Zorina, {Alla I} and Levin, {Aleksandr A} and Pereira, {Frederico D A S} and Petrov, {Stanislav V} and Karshieva, {Saida Sh} and Kasyanov, {Vladimir A} and Manturova, {Natalya E} and Ustyugov, {Andrey Yu} and Potekaev, {Nikolay N} and Parfenov, {Vladislav A} and Karalkin, {Pavel A} and Khesuani, {Yusef D} and Bulanova, {Elena A} and Kopnin, {Pavel B} and Isaev, {Artur A} and Mironov, {Vladimir A} and Zorin, {Vadim L}",

note = "Funding Information: This work was funded by the Ministry of Science and Higher Education of the Russian Federation under the strategic academic leadership program “Priority 2030” and supported by Russian Science Foundation (RSF) (project No. 20–15-00321). Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2022",

month = dec,

doi = "10.1007/s00441-022-03690-1",

language = "English",

volume = "390",

pages = "453--464",

journal = "Cell and Tissue Research",

issn = "0302-766X",

publisher = "Springer Verlag",

number = "3",

}

Koudan, EV, Zorina, AI, Levin, AA, Pereira, FDAS, Petrov, SV, Karshieva, SS, Kasyanov, VA, Manturova, NE, Ustyugov, AY, Potekaev, NN, Parfenov, VA, Karalkin, PA, Khesuani, YD, Bulanova, EA, Kopnin, PB, Isaev, AA, Mironov, VA & Zorin, VL 2022, 'Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids', Cell and Tissue Research, vol. 390, no. 3, pp. 453-464. https://doi.org/10.1007/s00441-022-03690-1

Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids. / Koudan, Elizaveta V (Corresponding Author); Zorina, Alla I; Levin, Aleksandr A et al.
In: Cell and Tissue Research, Vol. 390, No. 3, 12.2022, p. 453-464.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids

AU - Koudan, Elizaveta V

AU - Zorina, Alla I

AU - Levin, Aleksandr A

AU - Pereira, Frederico D A S

AU - Petrov, Stanislav V

AU - Karshieva, Saida Sh

AU - Kasyanov, Vladimir A

AU - Manturova, Natalya E

AU - Ustyugov, Andrey Yu

AU - Potekaev, Nikolay N

AU - Parfenov, Vladislav A

AU - Karalkin, Pavel A

AU - Khesuani, Yusef D

AU - Bulanova, Elena A

AU - Kopnin, Pavel B

AU - Isaev, Artur A

AU - Mironov, Vladimir A

AU - Zorin, Vadim L

N1 - Funding Information: This work was funded by the Ministry of Science and Higher Education of the Russian Federation under the strategic academic leadership program “Priority 2030” and supported by Russian Science Foundation (RSF) (project No. 20–15-00321). Publisher Copyright: © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2022/12

Y1 - 2022/12

N2 - In situ 3D bioprinting is a new emerging therapeutic modality for treating human skin diseases. The tissue spheroids have been previously suggested as a powerful tool in rapidly expanding bioprinting technology. It has been demonstrated that the regenerative potential of human dermal fibroblasts could be quantitatively evaluated in 2D cell culture and confirmed after implantation in vivo. However, the development of unbiassed quantitative criteria of the regenerative potential of 3D tissue spheroids in vitro before their in situ bioprinting remains to be investigated. Here it has been demonstrated for the first time that specific correlations exist between the regenerative potential of human dermal fibroblasts cultured in vitro as 2D cell monolayer with biological properties of 3D tissue spheroids fabricated from these fibroblasts. In vitro assessment of biological properties included diameter, spreading and fusion kinetics, and biomechanical properties of 3D tissue spheroids. This comprehensive characterization could be used to predict tissue spheroids' regenerative potential in vivo.

AB - In situ 3D bioprinting is a new emerging therapeutic modality for treating human skin diseases. The tissue spheroids have been previously suggested as a powerful tool in rapidly expanding bioprinting technology. It has been demonstrated that the regenerative potential of human dermal fibroblasts could be quantitatively evaluated in 2D cell culture and confirmed after implantation in vivo. However, the development of unbiassed quantitative criteria of the regenerative potential of 3D tissue spheroids in vitro before their in situ bioprinting remains to be investigated. Here it has been demonstrated for the first time that specific correlations exist between the regenerative potential of human dermal fibroblasts cultured in vitro as 2D cell monolayer with biological properties of 3D tissue spheroids fabricated from these fibroblasts. In vitro assessment of biological properties included diameter, spreading and fusion kinetics, and biomechanical properties of 3D tissue spheroids. This comprehensive characterization could be used to predict tissue spheroids' regenerative potential in vivo.

KW - Clonogenicity

KW - Dermal fibroblasts

KW - Proliferation

KW - Regenerative potential

KW - Tissue spheroids

UR - http://www.scopus.com/inward/record.url?scp=85138510471&partnerID=8YFLogxK

U2 - 10.1007/s00441-022-03690-1

DO - 10.1007/s00441-022-03690-1

M3 - Article

C2 - 36129531

SN - 0302-766X

VL - 390

SP - 453

EP - 464

JO - Cell and Tissue Research

JF - Cell and Tissue Research

IS - 3

ER -

Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids

Abstract

Keywords*

Field of Science*

Publication Type*

UN SDGs

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