Correlation of the regenerative potential of dermal fibroblasts in 2D culture with the biological properties of fibroblast-derived tissue spheroids

Elizaveta V Koudan (Coresponding Author), Alla I Zorina, Aleksandr A Levin, Frederico D A S Pereira, Stanislav V Petrov, Saida Sh Karshieva, Vladimir A Kasyanov, Natalya E Manturova, Andrey Yu Ustyugov, Nikolay N Potekaev, Vladislav A Parfenov, Pavel A Karalkin, Yusef D Khesuani, Elena A Bulanova, Pavel B Kopnin, Artur A Isaev, Vladimir A Mironov, Vadim L Zorin

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

In situ 3D bioprinting is a new emerging therapeutic modality for treating human skin diseases. The tissue spheroids have been previously suggested as a powerful tool in rapidly expanding bioprinting technology. It has been demonstrated that the regenerative potential of human dermal fibroblasts could be quantitatively evaluated in 2D cell culture and confirmed after implantation in vivo. However, the development of unbiassed quantitative criteria of the regenerative potential of 3D tissue spheroids in vitro before their in situ bioprinting remains to be investigated. Here it has been demonstrated for the first time that specific correlations exist between the regenerative potential of human dermal fibroblasts cultured in vitro as 2D cell monolayer with biological properties of 3D tissue spheroids fabricated from these fibroblasts. In vitro assessment of biological properties included diameter, spreading and fusion kinetics, and biomechanical properties of 3D tissue spheroids. This comprehensive characterization could be used to predict tissue spheroids' regenerative potential in vivo.

Original languageEnglish
Pages (from-to)453-464
Number of pages12
JournalCell and Tissue Research
Volume390
Issue number3
DOIs
Publication statusPublished - Dec 2022

Keywords*

  • Clonogenicity
  • Dermal fibroblasts
  • Proliferation
  • Regenerative potential
  • Tissue spheroids

Field of Science*

  • 3.2 Clinical medicine

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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