Activities per year
Abstract
One of missions of industrial biotechnology is to produce valuable
products from waste. In the current study we aim to establish a bioprocess
of syntrophic co-cultivation of two microorganisms (Crypthecodinium
cohnii and Zymomonas mobilis) at laboratory prototype level for
bioconversion of renewables (molasses, the byproduct of sugar industry,
and glycerol, byproduct of biodiesel production) into valuable vegetarian
(produced by microorganisms) docosahexaenoic acid (DHA) from omega 3
fatty acid family.
Cells of Z.mobilis strain (respiratory-deficient mutant derivative of the wild
type strain Z.mobilis ATCC 29191) will be immobilized, following the
previously described procedure of calcium alginate gel encapsulation.
Mathematical modelling will be used for different components and at
different stages of the syntrophic process to assist its improvement. The
syntrophic bioprocess will be optimized with respect to (i) the medium
composition, (ii) feed rates, (iii) oxygen supply, and (iv) the amounts of
added immobilized Z. mobilis cells at particular stages of the fermentation.
Genome and/or central carbon metabolism scale stoichiometric modelling
and optimization will be performed to increase the utilization of cheap
substrates and pre-treated residuals of C.cohnii. Bioprocess dynamics with
respect to growth speed of batch fermentations will be modelled to ensure
optimal proportions of syntrophic organisms, taking into account Z.mobilis
production and immobilization as “slave” process of C.cohnii cultivation.
Within several iterative cycles the fermentation data will be used for
modelling and computer simulation of the bioprocess, and at the same
time, the model-predicted dynamic behaviour of the system will aid further
improvement of fermentation performance.
products from waste. In the current study we aim to establish a bioprocess
of syntrophic co-cultivation of two microorganisms (Crypthecodinium
cohnii and Zymomonas mobilis) at laboratory prototype level for
bioconversion of renewables (molasses, the byproduct of sugar industry,
and glycerol, byproduct of biodiesel production) into valuable vegetarian
(produced by microorganisms) docosahexaenoic acid (DHA) from omega 3
fatty acid family.
Cells of Z.mobilis strain (respiratory-deficient mutant derivative of the wild
type strain Z.mobilis ATCC 29191) will be immobilized, following the
previously described procedure of calcium alginate gel encapsulation.
Mathematical modelling will be used for different components and at
different stages of the syntrophic process to assist its improvement. The
syntrophic bioprocess will be optimized with respect to (i) the medium
composition, (ii) feed rates, (iii) oxygen supply, and (iv) the amounts of
added immobilized Z. mobilis cells at particular stages of the fermentation.
Genome and/or central carbon metabolism scale stoichiometric modelling
and optimization will be performed to increase the utilization of cheap
substrates and pre-treated residuals of C.cohnii. Bioprocess dynamics with
respect to growth speed of batch fermentations will be modelled to ensure
optimal proportions of syntrophic organisms, taking into account Z.mobilis
production and immobilization as “slave” process of C.cohnii cultivation.
Within several iterative cycles the fermentation data will be used for
modelling and computer simulation of the bioprocess, and at the same
time, the model-predicted dynamic behaviour of the system will aid further
improvement of fermentation performance.
Original language | English |
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Pages | 70-70 |
Number of pages | 1 |
Publication status | Published - 12 Aug 2019 |
Externally published | Yes |
Event | Metabolic Pathway Analysis 2019 - University of Latvia, Riga, Latvia Duration: 12 Aug 2019 → 16 Aug 2019 https://www.biosystems.lv/mpa2019 |
Conference
Conference | Metabolic Pathway Analysis 2019 |
---|---|
Country/Territory | Latvia |
City | Riga |
Period | 12/08/19 → 16/08/19 |
Internet address |
Keywords*
- Systems biotechnology
- Syntropy
- Crypthecodinium cohnii
- Zymomonas mobilis
- Docosahexaenoic acid
Field of Science*
- 2.9 Industrial biotechnology
- 2.7 Environmental engineering
Publication Type*
- 3.4. Other publications in conference proceedings (including local)
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Metabolic Pathway Analysis 2019
Dāce, E. (Organising committee member)
12 Aug 2019 → 16 Aug 2019Activity: Participating in or organising an event types › Organising a conference, workshop, ...
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Crypthecodinium cohnii and Zymomonas mobilis syntrophy for production of omega 3 fatty acid
Dāce, E. (Speaker)
12 Aug 2019 → 16 Aug 2019Activity: Talk or presentation types › Poster presentation