Crypthecodinium cohnii and Zymomonas mobilis syntrophy for production of omega 3 fatty acid

Egils Stalidzāns, Agris Pentjušs, Elina Dace, Kristaps Bērziņš, Santa Prikule, Elīna Didrihsone, Juris Vanags, Uldis Kalnenieks

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One of missions of industrial biotechnology is to produce valuable
products from waste. In the current study we aim to establish a bioprocess
of syntrophic co-cultivation of two microorganisms (Crypthecodinium
cohnii and Zymomonas mobilis) at laboratory prototype level for
bioconversion of renewables (molasses, the byproduct of sugar industry,
and glycerol, byproduct of biodiesel production) into valuable vegetarian
(produced by microorganisms) docosahexaenoic acid (DHA) from omega 3
fatty acid family.
Cells of Z.mobilis strain (respiratory-deficient mutant derivative of the wild
type strain Z.mobilis ATCC 29191) will be immobilized, following the
previously described procedure of calcium alginate gel encapsulation.
Mathematical modelling will be used for different components and at
different stages of the syntrophic process to assist its improvement. The
syntrophic bioprocess will be optimized with respect to (i) the medium
composition, (ii) feed rates, (iii) oxygen supply, and (iv) the amounts of
added immobilized Z. mobilis cells at particular stages of the fermentation.
Genome and/or central carbon metabolism scale stoichiometric modelling
and optimization will be performed to increase the utilization of cheap
substrates and pre-treated residuals of C.cohnii. Bioprocess dynamics with
respect to growth speed of batch fermentations will be modelled to ensure
optimal proportions of syntrophic organisms, taking into account Z.mobilis
production and immobilization as “slave” process of C.cohnii cultivation.
Within several iterative cycles the fermentation data will be used for
modelling and computer simulation of the bioprocess, and at the same
time, the model-predicted dynamic behaviour of the system will aid further
improvement of fermentation performance.
Original languageEnglish
Number of pages1
Publication statusPublished - 12 Aug 2019
Externally publishedYes
EventMetabolic Pathway Analysis 2019 - University of Latvia, Riga, Latvia
Duration: 12 Aug 201916 Aug 2019


ConferenceMetabolic Pathway Analysis 2019
Internet address


  • Systems biotechnology
  • Syntropy
  • Crypthecodinium cohnii
  • Zymomonas mobilis
  • Docosahexaenoic acid

Field of Science*

  • 2.9 Industrial biotechnology
  • 2.7 Environmental engineering

Publication Type*

  • 3.4. Other publications in conference proceedings (including local)


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