Cytoskeleton systems contribute differently to the functional intrinsic properties of chondrospheres

Anna A. Gryadunova (Coresponding Author), Elizaveta V. Koudan, Sergey A. Rodionov, F. D.A.S. Pereira, Nina Yu Meteleva, Vladimir Kasyanov, Vladislav A. Parfenov, Alexey V. Kovalev, Yusef D. Khesuani, Vladimir A. Mironov, Elena A. Bulanova

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Cytoskeleton systems, actin microfilaments, microtubules (MTs) and intermediate filaments (IFs) provide the biomechanical stability and spatial organization in cells. To understand the specific contributions of each cytoskeleton systems to intrinsic properties of spheroids, we've scrutinized the effects of the cytoskeleton perturbants, cytochalasin D (Cyto D), nocodazole (Noc) and withaferin A (WFA) on fusion, spreading on adhesive surface, morphology and biomechanics of chondrospheres (CSs). We confirmed that treatment with Cyto D but not with Noc or WFA severely affected CSs fusion and spreading dynamics and significantly reduced biomechanical properties of cell aggregates. Noc treatment affected spheroids spreading but not the fusion and surprisingly enhanced their stiffness. Vimentin intermediate filaments (VIFs) reorganization affected CSs spreading only. The analysis of all three cytoskeleton systems contribution to spheroids intrinsic properties was performed for the first time.

Original languageEnglish
Pages (from-to)141-152
Number of pages12
JournalActa Biomaterialia
Publication statusPublished - 1 Dec 2020


  • Biomechanical properties
  • Chondrospheres
  • Cytoskeleton
  • Fusion
  • Morphology
  • Spreading

Field of Science*

  • 2.6 Medical engineering
  • 3.1 Basic medicine

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


Dive into the research topics of 'Cytoskeleton systems contribute differently to the functional intrinsic properties of chondrospheres'. Together they form a unique fingerprint.

Cite this