Cytoskeleton systems contribute differently to the functional intrinsic properties of chondrospheres

Anna A. Gryadunova; Elizaveta V. Koudan; Sergey A. Rodionov; F. D.A.S. Pereira; Nina Yu Meteleva; Vladimir Kasyanov; Vladislav A. Parfenov; Alexey V. Kovalev; Yusef D. Khesuani; Vladimir A. Mironov; Elena A. Bulanova

doi:10.1016/j.actbio.2020.10.007

Cytoskeleton systems contribute differently to the functional intrinsic properties of chondrospheres

Anna A. Gryadunova (Corresponding Author), Elizaveta V. Koudan, Sergey A. Rodionov, F. D.A.S. Pereira, Nina Yu Meteleva, Vladimir Kasyanov, Vladislav A. Parfenov, Alexey V. Kovalev, Yusef D. Khesuani, Vladimir A. Mironov, Elena A. Bulanova

Rīga Stradiņš University

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Cytoskeleton systems, actin microfilaments, microtubules (MTs) and intermediate filaments (IFs) provide the biomechanical stability and spatial organization in cells. To understand the specific contributions of each cytoskeleton systems to intrinsic properties of spheroids, we've scrutinized the effects of the cytoskeleton perturbants, cytochalasin D (Cyto D), nocodazole (Noc) and withaferin A (WFA) on fusion, spreading on adhesive surface, morphology and biomechanics of chondrospheres (CSs). We confirmed that treatment with Cyto D but not with Noc or WFA severely affected CSs fusion and spreading dynamics and significantly reduced biomechanical properties of cell aggregates. Noc treatment affected spheroids spreading but not the fusion and surprisingly enhanced their stiffness. Vimentin intermediate filaments (VIFs) reorganization affected CSs spreading only. The analysis of all three cytoskeleton systems contribution to spheroids intrinsic properties was performed for the first time.

Original language	English
Pages (from-to)	141-152
Number of pages	12
Journal	Acta Biomaterialia
Volume	118
DOIs	https://doi.org/10.1016/j.actbio.2020.10.007
Publication status	Published - 1 Dec 2020

Keywords*

Biomechanical properties
Chondrospheres
Cytoskeleton
Fusion
Morphology
Spreading

Field of Science*

2.6 Medical engineering
3.1 Basic medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1016/j.actbio.2020.10.007

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Gryadunova, A. A., Koudan, E. V., Rodionov, S. A., Pereira, F. D. A. S., Meteleva, N. Y., Kasyanov, V., Parfenov, V. A., Kovalev, A. V., Khesuani, Y. D., Mironov, V. A., & Bulanova, E. A. (2020). Cytoskeleton systems contribute differently to the functional intrinsic properties of chondrospheres. Acta Biomaterialia, 118, 141-152. https://doi.org/10.1016/j.actbio.2020.10.007

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title = "Cytoskeleton systems contribute differently to the functional intrinsic properties of chondrospheres",

abstract = "Cytoskeleton systems, actin microfilaments, microtubules (MTs) and intermediate filaments (IFs) provide the biomechanical stability and spatial organization in cells. To understand the specific contributions of each cytoskeleton systems to intrinsic properties of spheroids, we've scrutinized the effects of the cytoskeleton perturbants, cytochalasin D (Cyto D), nocodazole (Noc) and withaferin A (WFA) on fusion, spreading on adhesive surface, morphology and biomechanics of chondrospheres (CSs). We confirmed that treatment with Cyto D but not with Noc or WFA severely affected CSs fusion and spreading dynamics and significantly reduced biomechanical properties of cell aggregates. Noc treatment affected spheroids spreading but not the fusion and surprisingly enhanced their stiffness. Vimentin intermediate filaments (VIFs) reorganization affected CSs spreading only. The analysis of all three cytoskeleton systems contribution to spheroids intrinsic properties was performed for the first time.",

keywords = "Biomechanical properties, Chondrospheres, Cytoskeleton, Fusion, Morphology, Spreading",

author = "Gryadunova, {Anna A.} and Koudan, {Elizaveta V.} and Rodionov, {Sergey A.} and Pereira, {F. D.A.S.} and Meteleva, {Nina Yu} and Vladimir Kasyanov and Parfenov, {Vladislav A.} and Kovalev, {Alexey V.} and Khesuani, {Yusef D.} and Mironov, {Vladimir A.} and Bulanova, {Elena A.}",

note = "Funding Information: The reported study was funded by RFBR , project number 19-315-90017 . Publisher Copyright: {\textcopyright} 2020 Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = dec,

day = "1",

doi = "10.1016/j.actbio.2020.10.007",

language = "English",

volume = "118",

pages = "141--152",

journal = "Acta Biomaterialia",

issn = "1742-7061",

publisher = "Elsevier BV",

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TY - JOUR

T1 - Cytoskeleton systems contribute differently to the functional intrinsic properties of chondrospheres

AU - Gryadunova, Anna A.

AU - Koudan, Elizaveta V.

AU - Rodionov, Sergey A.

AU - Pereira, F. D.A.S.

AU - Meteleva, Nina Yu

AU - Kasyanov, Vladimir

AU - Parfenov, Vladislav A.

AU - Kovalev, Alexey V.

AU - Khesuani, Yusef D.

AU - Mironov, Vladimir A.

AU - Bulanova, Elena A.

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Cytoskeleton systems, actin microfilaments, microtubules (MTs) and intermediate filaments (IFs) provide the biomechanical stability and spatial organization in cells. To understand the specific contributions of each cytoskeleton systems to intrinsic properties of spheroids, we've scrutinized the effects of the cytoskeleton perturbants, cytochalasin D (Cyto D), nocodazole (Noc) and withaferin A (WFA) on fusion, spreading on adhesive surface, morphology and biomechanics of chondrospheres (CSs). We confirmed that treatment with Cyto D but not with Noc or WFA severely affected CSs fusion and spreading dynamics and significantly reduced biomechanical properties of cell aggregates. Noc treatment affected spheroids spreading but not the fusion and surprisingly enhanced their stiffness. Vimentin intermediate filaments (VIFs) reorganization affected CSs spreading only. The analysis of all three cytoskeleton systems contribution to spheroids intrinsic properties was performed for the first time.

AB - Cytoskeleton systems, actin microfilaments, microtubules (MTs) and intermediate filaments (IFs) provide the biomechanical stability and spatial organization in cells. To understand the specific contributions of each cytoskeleton systems to intrinsic properties of spheroids, we've scrutinized the effects of the cytoskeleton perturbants, cytochalasin D (Cyto D), nocodazole (Noc) and withaferin A (WFA) on fusion, spreading on adhesive surface, morphology and biomechanics of chondrospheres (CSs). We confirmed that treatment with Cyto D but not with Noc or WFA severely affected CSs fusion and spreading dynamics and significantly reduced biomechanical properties of cell aggregates. Noc treatment affected spheroids spreading but not the fusion and surprisingly enhanced their stiffness. Vimentin intermediate filaments (VIFs) reorganization affected CSs spreading only. The analysis of all three cytoskeleton systems contribution to spheroids intrinsic properties was performed for the first time.

KW - Biomechanical properties

KW - Chondrospheres

KW - Cytoskeleton

KW - Fusion

KW - Morphology

KW - Spreading

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U2 - 10.1016/j.actbio.2020.10.007

DO - 10.1016/j.actbio.2020.10.007

M3 - Article

C2 - 33045401

AN - SCOPUS:85092708257

SN - 1742-7061

VL - 118

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EP - 152

JO - Acta Biomaterialia

JF - Acta Biomaterialia

ER -

Cytoskeleton systems contribute differently to the functional intrinsic properties of chondrospheres

Abstract

Keywords*

Field of Science*

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