Demonstration of an atp-dependent proteolysis in etiolated pea extracts

Miki Nakazawa; Shinobu Satoh; Tadashi Fujii

doi:10.1016/0031-9422(91)84173-P

Demonstration of an atp-dependent proteolysis in etiolated pea extracts

Miki Nakazawa, Shinobu Satoh, Tadashi Fujii

Research output: Contribution to journal › Article › peer-review

Abstract

ATP-dependent proteolytic activity was detected in a crude extract of etiolated pea (Pisum sativum cv Alaska) epicotyls with phytochrome as a substrate. Only the far-red absorbing form of phytochrome served as the substrate for the protease. The activity was inhibited by diisopropyl fluorophosphate, leupeptin and antipain. ATP was required as a cofactor for the activity.

Original language	English
Pages (from-to)	1401-1403
Number of pages	3
Journal	Phytochemistry
Volume	30
Issue number	5
DOIs	https://doi.org/10.1016/0031-9422(91)84173-P
Publication status	Published - 1991
Externally published	Yes

Keywords*

ATP-dependence
Leguminosae
pea
phytochrome
Pisum sativum
proteolysis.

Field of Science*

1.6 Biological sciences
2.8 Environmental biotechnology

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

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10.1016/0031-9422(91)84173-P

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@article{c9ef832cf6a5492083f73acacc449747,

title = "Demonstration of an atp-dependent proteolysis in etiolated pea extracts",

abstract = "ATP-dependent proteolytic activity was detected in a crude extract of etiolated pea (Pisum sativum cv Alaska) epicotyls with phytochrome as a substrate. Only the far-red absorbing form of phytochrome served as the substrate for the protease. The activity was inhibited by diisopropyl fluorophosphate, leupeptin and antipain. ATP was required as a cofactor for the activity.",

keywords = "ATP-dependence, Leguminosae, pea, phytochrome, Pisum sativum, proteolysis.",

author = "Miki Nakazawa and Shinobu Satoh and Tadashi Fujii",

note = "Funding Information: Acknowledgements-The authors thank Dr M. Furuya and Dr A. Nagatani for helpful discussions and for providing the anti-phytochrome specific antibodies. This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education (63621002).",

year = "1991",

doi = "10.1016/0031-9422(91)84173-P",

language = "English",

volume = "30",

pages = "1401--1403",

journal = "Phytochemistry",

issn = "0031-9422",

publisher = "Elsevier Ltd.",

number = "5",

}

TY - JOUR

T1 - Demonstration of an atp-dependent proteolysis in etiolated pea extracts

AU - Nakazawa, Miki

AU - Satoh, Shinobu

AU - Fujii, Tadashi

N1 - Funding Information: Acknowledgements-The authors thank Dr M. Furuya and Dr A. Nagatani for helpful discussions and for providing the anti-phytochrome specific antibodies. This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education (63621002).

PY - 1991

Y1 - 1991

N2 - ATP-dependent proteolytic activity was detected in a crude extract of etiolated pea (Pisum sativum cv Alaska) epicotyls with phytochrome as a substrate. Only the far-red absorbing form of phytochrome served as the substrate for the protease. The activity was inhibited by diisopropyl fluorophosphate, leupeptin and antipain. ATP was required as a cofactor for the activity.

AB - ATP-dependent proteolytic activity was detected in a crude extract of etiolated pea (Pisum sativum cv Alaska) epicotyls with phytochrome as a substrate. Only the far-red absorbing form of phytochrome served as the substrate for the protease. The activity was inhibited by diisopropyl fluorophosphate, leupeptin and antipain. ATP was required as a cofactor for the activity.

KW - ATP-dependence

KW - Leguminosae

KW - pea

KW - phytochrome

KW - Pisum sativum

KW - proteolysis.

UR - http://www.scopus.com/inward/record.url?scp=44949281147&partnerID=8YFLogxK

U2 - 10.1016/0031-9422(91)84173-P

DO - 10.1016/0031-9422(91)84173-P

M3 - Article

AN - SCOPUS:44949281147

SN - 0031-9422

VL - 30

SP - 1401

EP - 1403

JO - Phytochemistry

JF - Phytochemistry

IS - 5

ER -

Demonstration of an atp-dependent proteolysis in etiolated pea extracts

Abstract

Keywords*

Field of Science*

Publication Type*

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