TY - JOUR
T1 - Design of Liposomes Carrying Helixcomplex Snail Mucus
T2 - Preliminary Studies
AU - Alogna, Andrea
AU - Gentili, Valentina
AU - Trapella, Claudio
AU - Hallan, Supandeep Singh
AU - Sguizzato, Maddalena
AU - Strazzabosco, Giovanni
AU - Fernández, Mercedes
AU - Cortesi, Rita
AU - Rizzo, Roberta
AU - Bortolotti, Daria
N1 - Funding Information:
This research was funded by Camera di Commercio of Ferrara (CCIAA2018, R.R.; CCIAA2019, D.B.); Project ARS01_01163. HelixRec-HelixReCovery PON ?Research and Innovation? 2014?2020; FSC and University of Ferrara FAR2019, FAR2020 (R.C., C.T., R.R.).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/8/2
Y1 - 2021/8/2
N2 - In recent decades liposomes have been used in different field thanks to their ability to act as a vehicle for a wide range of biomolecules, their great versatility and their easy production. The aim of this study was to evaluate liposomes as a vehicle for the actives present in the HelixComplex (HC) snail mucus for topical delivery. Liposomes composed of a mixture of phosphatidylcholine, cholesterol and octadecylamine were prepared with and without HC (empty liposomes) and their biological efficacy was tested by evaluating cell viability and migration. HC-loaded liposomes (LHC) were stable throughout 60 days of observation, and showed interesting effects on wound healing reconstitution. In particular, we observed that 25 µg/mL LHC were already able to induce a higher cell monolayer reconstitution in comparison to the untreated samples and HC treated samples after only 4 h (28% versus 10% and 7%, p = 0.03 and p= 0.003, respectively). The effect was more evident at 24 h in comparison with the untreated control (54% versus 21.2% and 41.6%, p = 0.006 and p = NS, respectively). These results represent a preliminary, but promising, novelty in the delivery strategy of the actives present in the HelixComplex mucus.
AB - In recent decades liposomes have been used in different field thanks to their ability to act as a vehicle for a wide range of biomolecules, their great versatility and their easy production. The aim of this study was to evaluate liposomes as a vehicle for the actives present in the HelixComplex (HC) snail mucus for topical delivery. Liposomes composed of a mixture of phosphatidylcholine, cholesterol and octadecylamine were prepared with and without HC (empty liposomes) and their biological efficacy was tested by evaluating cell viability and migration. HC-loaded liposomes (LHC) were stable throughout 60 days of observation, and showed interesting effects on wound healing reconstitution. In particular, we observed that 25 µg/mL LHC were already able to induce a higher cell monolayer reconstitution in comparison to the untreated samples and HC treated samples after only 4 h (28% versus 10% and 7%, p = 0.03 and p= 0.003, respectively). The effect was more evident at 24 h in comparison with the untreated control (54% versus 21.2% and 41.6%, p = 0.006 and p = NS, respectively). These results represent a preliminary, but promising, novelty in the delivery strategy of the actives present in the HelixComplex mucus.
KW - Drug delivery
KW - HelixComplex
KW - Liposomes
KW - MTT test
KW - Slime mucus
UR - http://www.scopus.com/inward/record.url?scp=85112111450&partnerID=8YFLogxK
U2 - 10.3390/molecules26164709
DO - 10.3390/molecules26164709
M3 - Article
C2 - 34443296
AN - SCOPUS:85112111450
SN - 1420-3049
VL - 26
JO - Molecules
JF - Molecules
IS - 16
M1 - 4709
ER -