Design of Nanosystems for the Delivery of Quorum Sensing Inhibitors: A preliminary study

Supandeep Singh Hallan, Paolo Marchetti, Daria Bortolotti, Maddalena Sguizzato, Elisabetta Esposito (Coresponding Author), Paolo Mariani, Claudio Trapella, Roberta Rizzo, Rita Cortesi (Coresponding Author)

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Biofilm production is regulated by the Quorum Sensing system. Nowadays, Quorum Sensing represents an appealing target to design new compounds to increase antibiotics effects and avoid development of antibiotics multiresistance. In this research the use of liposomes to target two novel synthetic biofilm inhibitors is presented, focusing on a preformulation study to select a liposome composition for in vitro test. Five different liposome (LP) formulations, composed of phosphatidyl choline, cholesterol and charged surfactant (2:1:1, molar ratio) have been prepared by direct hydration and extrusion. As charged surfactants dicetyl phosphate didecyldimethylammonium chloride, di isobutyl phenoxy ethyl dimethyl benzyl ammonium chloride and stearylamine (SA) and have been used. Liposome charge, size and morphology were investigated by zeta potential, photon correlation spectroscopy, small angle x-ray spectroscopy and electron microscopy. LP-SA was selected for the loading of biofilm inhibitors and subjected to high performance liquid chromatography for entrapment capacity evaluation. LP-SA loaded inhibitors showed a higher diameter (223.6 nm) as compared to unloaded ones (205.7 nm) and a dose-dependent anti-biofilm effect mainly after 48 h of treatment, while free biofilm inhibitors loose activity. In conclusion, our data supported the use of liposomes as a strategy to enhance biofilm inhibitors effect.

Original languageEnglish
Article number5655
JournalMolecules
Volume25
Issue number23
DOIs
Publication statusPublished - Dec 2020
Externally publishedYes

Keywords*

  • Biofilm
  • Drug delivery
  • Liposomes
  • MTT test
  • Nanotechnological systems
  • QS inhibitors

Field of Science*

  • 1.4 Chemical sciences
  • 3.1 Basic medicine

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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