Designing of Combined Nano and Microfiber Network by Immobilization of Oxidized Cellulose Nanofiber on Polycaprolactone Fibrous Scaffold

In this study, the immobilization of oxidized cellulose nanofiber (OCNF) network on the polycaprolactone (PCL) fibrous scaffold was performed by the aminolysis procedure through electrospinning and layer by layer (LBL) techniques. The morphology of the fibrous scaffold was examined by field emission scanning electron microscopy (FE-SEM), and it indicated that after immobilization of OCNF on PCL, the unique nanofiber and nano network was created. Moreover, the physical and chemical properties of samples were examined using X-ray photoelectron spectroscopy (XPS), Fourier transform spectroscopy (FT-IR), thermogravimetric analysis (TGA), water contact angle measurement, and BSA adsorption properties. Furthermore, the cellular responses to PCL scaffold with and without modification by OCNF were examined by seeding rat bone marrow stem cells (RBMSCs) on the fibrous scaffold for assessing cell attachment, cell viability, and proliferation. Thus, the present study focused on preparation and characterization of a membrane and tubular scaffold with a unique nanostructure, which is an excellent candidate for use as a blood vessel scaffold graft.