Abstract
Background: Orofacial clefts are one of the most common birth defects with multifactorial and only partly understood morphopathogenesis. Objective: The aim of this study was to evaluate the presence of TGF-β1, HGF, IGF-1 and IGF-1R in cleft affected mucosa of the lip. Methods: Lip mucosa tissue samples were obtained during surgical cleft correction from seven 2 to 6 months old children. Prepared tissue sections were stained by immunohistochemistry for TGF-β1, HGF, IGF-1 and IGF-1R. The intensity of staining was graded semiquantitatively. Results: We found numerous TGF-β1 and HGF-containing epithelial and connective tissue cells, moderate number of IGF-1 immunoreactive cells and even less pronounced presence of IGF-1R-positive cells. Conclusion: TGF-β1 and HGF are present in defective epithelia and soft tissue in cleft affected lip. Expressions of IGF-1 and IGF-1R show significant differences, and both factors play a role in the morphopathogenesis of clefts.
| Original language | English |
|---|---|
| Pages (from-to) | 46-52 |
| Number of pages | 7 |
| Journal | Open Dermatology Journal |
| Volume | 11 |
| DOIs | |
| Publication status | Published - 1 Sept 2017 |
Keywords*
- Cleft
- HGF
- IGF-1
- IGF-1R
- Lip mucosa
- Orofacial defects
- TGF-β1
Field of Science*
- 3.1 Basic medicine
- 3.2 Clinical medicine
Publication Type*
- 1.1. Scientific article indexed in Web of Science and/or Scopus database