TY - JOUR
T1 - Determination of Hormone Antagonists in Waste-Water Samples by Micellar Electrokinetic Chromatography
AU - Rucins, Martins
AU - Baron, Daniel
AU - Plotniece, Aiva
AU - Petr, Jan
N1 - Funding Information:
Acknowledgements The financial support of the research by the projects NPU LO 1305 (Ministry of Education, Youth and Sports of the Czech Republic), IGA_PrF_2018_027 (Palacký University in Olomouc, Czech Republic), and InnovaBalt (REGPOT-CT-2013-316149) project for M. Rucins is gratefully acknowledged.
Publisher Copyright:
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - The contamination of water represents a major problem in many parts of the world. Endocrine disrupting compounds belong to the water contaminants and affect, besides other things, hormonal equilibrium in the living organisms. Such compounds are of different chemical structures and there is a demand for their fast determination in water samples. In our work, we focused on the development of a simple and fast method for separation and determination of five model hormone antagonists, exemestane, toremifene, letrozole, anastrozole, and mifepristone, by micellar electrokinetic chromatography. Within the best conditions, 50 mM sodium borate pH 9.5, 50 mM SDS, 15% 1-propanol, 20 kV, the separation of all the compounds lasted 8 min. The method was successfully validated with LODs of 1.2–7.7 µg mL−1, LOQs of 4.0–25.6 µg mL−1 and intraday repeatability of peak areas of 0.8–1.1%. Moreover, it was applied to the analysis of spiked water samples from a local waste-water treatment plant using SPE as a pretreatment step.
AB - The contamination of water represents a major problem in many parts of the world. Endocrine disrupting compounds belong to the water contaminants and affect, besides other things, hormonal equilibrium in the living organisms. Such compounds are of different chemical structures and there is a demand for their fast determination in water samples. In our work, we focused on the development of a simple and fast method for separation and determination of five model hormone antagonists, exemestane, toremifene, letrozole, anastrozole, and mifepristone, by micellar electrokinetic chromatography. Within the best conditions, 50 mM sodium borate pH 9.5, 50 mM SDS, 15% 1-propanol, 20 kV, the separation of all the compounds lasted 8 min. The method was successfully validated with LODs of 1.2–7.7 µg mL−1, LOQs of 4.0–25.6 µg mL−1 and intraday repeatability of peak areas of 0.8–1.1%. Moreover, it was applied to the analysis of spiked water samples from a local waste-water treatment plant using SPE as a pretreatment step.
KW - Endocrine disruptors
KW - Micellar electrokinetic chromatography
KW - Waste-water
UR - http://www.scopus.com/inward/record.url?scp=85055708186&partnerID=8YFLogxK
U2 - 10.1007/s10337-018-3631-0
DO - 10.1007/s10337-018-3631-0
M3 - Article
AN - SCOPUS:85055708186
SN - 0009-5893
VL - 81
SP - 1607
EP - 1612
JO - Chromatographia
JF - Chromatographia
IS - 12
ER -