Determination of Hormone Antagonists in Waste-Water Samples by Micellar Electrokinetic Chromatography

Martins Rucins, Daniel Baron, Aiva Plotniece, Jan Petr (Coresponding Author)

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


The contamination of water represents a major problem in many parts of the world. Endocrine disrupting compounds belong to the water contaminants and affect, besides other things, hormonal equilibrium in the living organisms. Such compounds are of different chemical structures and there is a demand for their fast determination in water samples. In our work, we focused on the development of a simple and fast method for separation and determination of five model hormone antagonists, exemestane, toremifene, letrozole, anastrozole, and mifepristone, by micellar electrokinetic chromatography. Within the best conditions, 50 mM sodium borate pH 9.5, 50 mM SDS, 15% 1-propanol, 20 kV, the separation of all the compounds lasted 8 min. The method was successfully validated with LODs of 1.2–7.7 µg mL−1, LOQs of 4.0–25.6 µg mL−1 and intraday repeatability of peak areas of 0.8–1.1%. Moreover, it was applied to the analysis of spiked water samples from a local waste-water treatment plant using SPE as a pretreatment step.

Original languageEnglish
Pages (from-to)1607-1612
Number of pages6
Issue number12
Publication statusPublished - 1 Dec 2018
Externally publishedYes


  • Endocrine disruptors
  • Micellar electrokinetic chromatography
  • Waste-water

Field of Science*

  • 1.4 Chemical sciences
  • 1.6 Biological sciences

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


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