Determination of trimethylamine-N-oxide in combination with l-carnitine and γ-butyrobetaine in human plasma by UPLC/MS/MS

Solveiga Grinberga, Maija Dambrova, Gustavs Latkovskis, Ieva Strele, Ilze Konrade, Dace Hartmane, Eduards Sevostjanovs, Edgars Liepinsh, Osvalds Pugovics

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

An ultra-high-performance liquid chromatography-mass spectrometry (UPLC/MS/MS) method was developed and validated for the quantification of trimethylamine-N-oxide (TMAO) simultaneously with TMAO-related molecules l-carnitine and γ-butyrobetaine (GBB) in human blood plasma. The separation of analytes was achieved using a Hydrophilic interaction liquid chromatography (HILIC)-type column with ammonium acetate-acetonitrile as the mobile phase. TMAO determination was validated according to valid US Food and Drug Administration guidelines. The developed method was successfully applied to plasma samples from healthy volunteers.

Original languageEnglish
Pages (from-to)1670-1674
Number of pages5
JournalBiomedical Chromatography
Volume29
Issue number11
DOIs
Publication statusPublished - Nov 2015

Keywords

  • Human plasma
  • L-carnitine
  • Trimethylamine N-oxide
  • UPLC/MS/MS
  • γ-butyrobetaine

Field of Science

  • 1.4 Chemical sciences
  • 1.6 Biological sciences
  • 3.1 Basic medicine

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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