Determination of trimethylamine-N-oxide in combination with l-carnitine and γ-butyrobetaine in human plasma by UPLC/MS/MS

Solveiga Grinberga; Maija Dambrova; Gustavs Latkovskis; Ieva Strele; Ilze Konrade; Dace Hartmane; Eduards Sevostjanovs; Edgars Liepinsh; Osvalds Pugovics

doi:10.1002/bmc.3477

Determination of trimethylamine-N-oxide in combination with l-carnitine and γ-butyrobetaine in human plasma by UPLC/MS/MS

Solveiga Grinberga, Maija Dambrova, Gustavs Latkovskis, Ieva Strele, Ilze Konrade, Dace Hartmane, Eduards Sevostjanovs, Edgars Liepinsh, Osvalds Pugovics

Rīga Stradiņš University

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

An ultra-high-performance liquid chromatography-mass spectrometry (UPLC/MS/MS) method was developed and validated for the quantification of trimethylamine-N-oxide (TMAO) simultaneously with TMAO-related molecules l-carnitine and γ-butyrobetaine (GBB) in human blood plasma. The separation of analytes was achieved using a Hydrophilic interaction liquid chromatography (HILIC)-type column with ammonium acetate-acetonitrile as the mobile phase. TMAO determination was validated according to valid US Food and Drug Administration guidelines. The developed method was successfully applied to plasma samples from healthy volunteers.

Original language	English
Pages (from-to)	1670-1674
Number of pages	5
Journal	Biomedical Chromatography
Volume	29
Issue number	11
DOIs	https://doi.org/10.1002/bmc.3477
Publication status	Published - Nov 2015

Keywords*

Human plasma
L-carnitine
Trimethylamine N-oxide
UPLC/MS/MS
γ-butyrobetaine

Field of Science*

1.4 Chemical sciences
1.6 Biological sciences
3.1 Basic medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/bmc.3477

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title = "Determination of trimethylamine-N-oxide in combination with l-carnitine and γ-butyrobetaine in human plasma by UPLC/MS/MS",

abstract = "An ultra-high-performance liquid chromatography-mass spectrometry (UPLC/MS/MS) method was developed and validated for the quantification of trimethylamine-N-oxide (TMAO) simultaneously with TMAO-related molecules l-carnitine and γ-butyrobetaine (GBB) in human blood plasma. The separation of analytes was achieved using a Hydrophilic interaction liquid chromatography (HILIC)-type column with ammonium acetate-acetonitrile as the mobile phase. TMAO determination was validated according to valid US Food and Drug Administration guidelines. The developed method was successfully applied to plasma samples from healthy volunteers.",

keywords = "Human plasma, L-carnitine, Trimethylamine N-oxide, UPLC/MS/MS, γ-butyrobetaine",

author = "Solveiga Grinberga and Maija Dambrova and Gustavs Latkovskis and Ieva Strele and Ilze Konrade and Dace Hartmane and Eduards Sevostjanovs and Edgars Liepinsh and Osvalds Pugovics",

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year = "2015",

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doi = "10.1002/bmc.3477",

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volume = "29",

pages = "1670--1674",

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T1 - Determination of trimethylamine-N-oxide in combination with l-carnitine and γ-butyrobetaine in human plasma by UPLC/MS/MS

AU - Grinberga, Solveiga

AU - Dambrova, Maija

AU - Latkovskis, Gustavs

AU - Strele, Ieva

AU - Konrade, Ilze

AU - Hartmane, Dace

AU - Sevostjanovs, Eduards

AU - Liepinsh, Edgars

AU - Pugovics, Osvalds

PY - 2015/11

Y1 - 2015/11

N2 - An ultra-high-performance liquid chromatography-mass spectrometry (UPLC/MS/MS) method was developed and validated for the quantification of trimethylamine-N-oxide (TMAO) simultaneously with TMAO-related molecules l-carnitine and γ-butyrobetaine (GBB) in human blood plasma. The separation of analytes was achieved using a Hydrophilic interaction liquid chromatography (HILIC)-type column with ammonium acetate-acetonitrile as the mobile phase. TMAO determination was validated according to valid US Food and Drug Administration guidelines. The developed method was successfully applied to plasma samples from healthy volunteers.

AB - An ultra-high-performance liquid chromatography-mass spectrometry (UPLC/MS/MS) method was developed and validated for the quantification of trimethylamine-N-oxide (TMAO) simultaneously with TMAO-related molecules l-carnitine and γ-butyrobetaine (GBB) in human blood plasma. The separation of analytes was achieved using a Hydrophilic interaction liquid chromatography (HILIC)-type column with ammonium acetate-acetonitrile as the mobile phase. TMAO determination was validated according to valid US Food and Drug Administration guidelines. The developed method was successfully applied to plasma samples from healthy volunteers.

KW - Human plasma

KW - L-carnitine

KW - Trimethylamine N-oxide

KW - UPLC/MS/MS

KW - γ-butyrobetaine

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U2 - 10.1002/bmc.3477

DO - 10.1002/bmc.3477

M3 - Article

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SN - 0269-3879

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SP - 1670

EP - 1674

JO - Biomedical Chromatography

JF - Biomedical Chromatography

IS - 11

ER -

Determination of trimethylamine-N-oxide in combination with l-carnitine and γ-butyrobetaine in human plasma by UPLC/MS/MS

Abstract

Keywords*

Field of Science*

Publication Type*

UN SDGs

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