Diagnosis of nitrosative stress by quantitative EPR-spectroscopy of epidermal cells

V. I. Petukhov, L. K. Baumane, E. D. Reste, T. Y. Zvagule, M. A. Romanova, N. I. Shushkevich, L. T. Sushkova, S. V. Skavronsky, A. N. Shchukov

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Quantitative assay of nitric oxide (NO) and iron (Fe) was carried out by the electron paramagnetic resonance (EPR) method in epidermal derivative (hair) of 45 liquidators of the Chernobyl accident, 30 employees of radiological institutions having contact with radiation, and 50 students of Vladimir State University, most of which were diagnosed with iron deficiency, namely iron deficiency anemia (4 persons) and latent iron deficiency (34 persons). Measurements were performed on a Radiopan EPR-spectrometer. It was found that the method of quantitative EPR-spectroscopy using diethyldithiocarbamate as a trap in vitro could be successfully used to measure NO-radical activity in such a biological substrate as hair. It was found that the intensity of NO-radical signal in the spectrogram depends on the Fe level (not only in the analyzed substrate, but also in the whole organism).

Original languageEnglish
Pages (from-to)734-736
Number of pages3
JournalBulletin of Experimental Biology and Medicine
Volume154
Issue number6
DOIs
Publication statusPublished - Apr 2013

Keywords

  • Chernobyl
  • EPR-spectroscopy
  • Fe deficiency
  • nitric oxide (NO)

Field of Science

  • 1.6 Biological sciences
  • 3.1 Basic medicine

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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