Distinct B cell profiles characterise healthy weight and obesity pre- and post-bariatric surgery

Baiba Šlisere, Marina Arisova, Olga Aizbalte, Marta Salmiņa, Maksims Zolovs, Maksims Mukāns, Mārtiņš Lēvenšteins, Igors Troickis, Laila Meija, Aivars Lejnieks, Guna Bīlande, Lizzy Rosser, Kristīne Oļeiņika (Coresponding Author)

Research output: Contribution to journalArticle


Obesity-associated metabolic dysfunction and inflammation can be ameliorated by bariatric surgery. While obesity is also linked to impaired B cell activation, differentiation, and persistence in response to infection and vaccination little is known about post-operative immune B cell compartment and to what extent dysregulation in B cell pathways can be reversed. To bridge this gap in knowledge, we carried out in-depth evaluation of B cell composition in obese individuals prior to and following bariatric surgery compared to lean controls.

We recruited individuals with obesity (BMI at least 35 kg/m2) before bariatric surgery (n = 21) and followed them up 6 months post-operatively (n = 17). As controls we recruited age- and sex-matched lean (BMI < 25) individuals (n = 18). We carried out comprehensive immunophenotyping of peripheral blood B cells as well as interrogated their association with inflammatory and metabolic parameters.

In obesity the balance of antigen-inexperienced and memory B cells in the peripheral blood is altered, with an expansion of naïve and a reduction in total memory B cells. 6 months following bariatric surgery this balance is restored. However, post-operative patients are uniquely characterised by an increase in B cell subsets associated with chronic inflammation – CD11c+CXCR5−IgD−CD27− double negative 2 (DN2) B cells and CD27+CD38++ plasmablasts. Correlations between B cells subsets, inflammatory and metabolic parameters were distinct in lean and obese individuals pre- and post-bariatric surgery.

Bariatric surgery patients display a unique B cell profile 6 months post-operatively; this bears minimal resemblance to that of pre-operative patients and only partially overlaps with that of lean controls. Post-operative differences in the B cell compartment compared to lean controls are detected despite global amelioration of inflammation and restoration of metabolic health. Collectively, this indicates that bariatric surgery creates a specific immunometabolic state with potential implications for health outcomes.
Original languageEnglish
Number of pages18
JournalInternational Journal of Obesity
Publication statusSubmitted - 20 Dec 2022

Field of Science*

  • 3.1 Basic medicine

Publication Type*

  • 6. Other publications


Dive into the research topics of 'Distinct B cell profiles characterise healthy weight and obesity pre- and post-bariatric surgery'. Together they form a unique fingerprint.

Cite this