Distribution of Growth Stimulating and Degradation Factors in Cow Endometrium in Postparturition Period: Augšanu stimulējošo un degradācijas faktoru izplatība govs endometrijā pēcdzemdību periodā

The aim of the research was to reveal distribution of the growth stimulating and degradation factors, inflammatory factors, and neuropeptide-containing innervation, as well as apoptosis in the cow’s endometrium in postparturition period. Haematoxylin and eosin were used for routine staining of each sample. Matrix
metalloproteinases type 2 and type 9 (MMP-2 un MMP-9, working dilution (w.d.) 1:100, R&D, England), tumor necrosis factor-α (TNF-α, w.d. 1:100, Abcam, England), interleukin-10 (IL-10, w.d. 1:400, Abcam, England), vascular endothelial growth factor (VEGF, w.d. 1:50, DakoCytomation, Denmark), nerve growth
factor receptors p75 (NGFR p75, w.d. 1:150, DakoCytomation, Denmark), and protein gene product 9.5 (PGP
9.5, w.d. 1:1600, DakoCytomation, Denmark) were detected immunohistochemically (IMH). TUNEL method
was used for detection of apoptosis (programmed cells death – PCD). A mild positive correlation (p<0.05) was observed between amount of inflammatory cells and TNF-α (r=0.52), PCD and TNF-α (r= 0.58), TNF-α and NGFR p75 (r=0.51), NGFR p75 and PGP 9.5 (r=0.49), VEGF and PCD (r=0.59), and VEGF and NGFR p75 (r=0.64). A strong positive correlation (p<0.01) was observed between TNF-α and VEGF (p<0.01; r=0.72), as well as between VEGF and NGFR p75 (r=0.64). The research suggests that uterus endometrial tissue degradation and repairing were in balance during postparturition. The intensity level of tissue repairing process in the cow’s endometrium was specific for the individual organism, which might be connected with immunity of each of Them.