Dynamic Laser Speckle Imaging for Fast Evaluation of the Antibacterial Susceptibility by the Disc Diffusion Method

Ilze Lihacova; Ilya Balmages; Aigars Reinis; Svjatoslavs Kistkins; Dmitrijs Bliznuks; Emilija V. Plorina; Alexey Lihachev

doi:10.1007/978-3-031-37132-5_39

Dynamic Laser Speckle Imaging for Fast Evaluation of the Antibacterial Susceptibility by the Disc Diffusion Method

Ilze Lihacova (Coresponding Author), Ilya Balmages, Aigars Reinis, Svjatoslavs Kistkins, Dmitrijs Bliznuks, Emilija V. Plorina, Alexey Lihachev

Department of Biology and Microbiology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Phenotypic resistance tests e.g., disc diffusion method require 16–24 h to obtain the results, while the PCR tests provide only genotypic type of antibacterial resistance. New methods are needed to assess antibacterial resistance faster than existing methods, thus providing targeted pharmacological intervention at the early stage of the disease, increasing a patient's survival chances. Laser speckle imaging technique allows tracking moving particles in optically inhomogeneous media, allowing to observe the processes of changing the behavior of the sterile zone. The choice and adaptation of a mathematical model describing changes in the growth diameter of the sterile zone as a function of time and comparison with experimental data will help to understand and describe the formation of the sterile zone. The aim is to create processing algorithms for the obtained images to predict the diameter at the end of the disc diffusion test from the diameter of the earliest visible sterile zone.

Original language	English
Title of host publication	19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics. NBC 2023.
Subtitle of host publication	Proceedings of NBC 2023
Editors	Yuri Dekhtyar, Inga Saknite
Publisher	Springer
Pages	313 -319
Volume	89
ISBN (Electronic)	978-3-031-37132-5
ISBN (Print)	978-3-031-37131-8
DOIs	https://doi.org/10.1007/978-3-031-37132-5_39
Publication status	Published - 16 Jul 2023
Event	19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics - Liepaja, Latvia Duration: 12 Jun 2023 → 14 Jun 2023 https://nbc2023.lmifb.lv/ https://ebooks.rtu.lv/product/19-ziemelvalstu-un-baltijas-biomedicinas-inzenierzinatnu-un-medicinas-fizikas-konference/

Publication series

Name	IFMBE Proceedings
Volume	89

Conference

Conference	19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics
Abbreviated title	NBC2023
Country/Territory	Latvia
City	Liepaja
Period	12/06/23 → 14/06/23
Other	19. Ziemeļvalstu un Baltijas Biomedicīnas inženierzinātņu un medicīnas fizikas konference
Internet address	https://nbc2023.lmifb.lv/ https://ebooks.rtu.lv/product/19-ziemelvalstu-un-baltijas-biomedicinas-inzenierzinatnu-un-medicinas-fizikas-konference/

Field of Science*

1.6 Biological sciences
3.1 Basic medicine
3.4 Medical biotechnology

Publication Type*

3.2. Articles or chapters in other proceedings other than those included in 3.1., with an ISBN or ISSN code

Access to Document

10.1007/978-3-031-37132-5_39

Cite this

Lihacova, I., Balmages, I., Reinis, A., Kistkins, S., Bliznuks, D., Plorina, E. V., & Lihachev, A. (2023). Dynamic Laser Speckle Imaging for Fast Evaluation of the Antibacterial Susceptibility by the Disc Diffusion Method. In Y. Dekhtyar, & I. Saknite (Eds.), 19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics. NBC 2023.: Proceedings of NBC 2023 (Vol. 89, pp. 313 -319). (IFMBE Proceedings; Vol. 89). Springer. https://doi.org/10.1007/978-3-031-37132-5_39

Lihacova, Ilze ; Balmages, Ilya ; Reinis, Aigars et al. / Dynamic Laser Speckle Imaging for Fast Evaluation of the Antibacterial Susceptibility by the Disc Diffusion Method. 19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics. NBC 2023.: Proceedings of NBC 2023. editor / Yuri Dekhtyar ; Inga Saknite. Vol. 89 Springer, 2023. pp. 313 -319 (IFMBE Proceedings).

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title = "Dynamic Laser Speckle Imaging for Fast Evaluation of the Antibacterial Susceptibility by the Disc Diffusion Method",

abstract = "Phenotypic resistance tests e.g., disc diffusion method require 16–24 h to obtain the results, while the PCR tests provide only genotypic type of antibacterial resistance. New methods are needed to assess antibacterial resistance faster than existing methods, thus providing targeted pharmacological intervention at the early stage of the disease, increasing a patient's survival chances. Laser speckle imaging technique allows tracking moving particles in optically inhomogeneous media, allowing to observe the processes of changing the behavior of the sterile zone. The choice and adaptation of a mathematical model describing changes in the growth diameter of the sterile zone as a function of time and comparison with experimental data will help to understand and describe the formation of the sterile zone. The aim is to create processing algorithms for the obtained images to predict the diameter at the end of the disc diffusion test from the diameter of the earliest visible sterile zone.",

author = "Ilze Lihacova and Ilya Balmages and Aigars Reinis and Svjatoslavs Kistkins and Dmitrijs Bliznuks and Plorina, {Emilija V.} and Alexey Lihachev",

year = "2023",

month = jul,

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doi = "10.1007/978-3-031-37132-5_39",

language = "English",

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series = "IFMBE Proceedings",

publisher = "Springer",

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booktitle = "19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics. NBC 2023.",

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note = "19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC2023 ; Conference date: 12-06-2023 Through 14-06-2023",

url = "https://nbc2023.lmifb.lv/, https://ebooks.rtu.lv/product/19-ziemelvalstu-un-baltijas-biomedicinas-inzenierzinatnu-un-medicinas-fizikas-konference/",

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Lihacova, I, Balmages, I, Reinis, A, Kistkins, S, Bliznuks, D, Plorina, EV & Lihachev, A 2023, Dynamic Laser Speckle Imaging for Fast Evaluation of the Antibacterial Susceptibility by the Disc Diffusion Method. in Y Dekhtyar & I Saknite (eds), 19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics. NBC 2023.: Proceedings of NBC 2023. vol. 89, IFMBE Proceedings, vol. 89, Springer, pp. 313 -319, 19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, Liepaja, Latvia, 12/06/23. https://doi.org/10.1007/978-3-031-37132-5_39

Dynamic Laser Speckle Imaging for Fast Evaluation of the Antibacterial Susceptibility by the Disc Diffusion Method. / Lihacova, Ilze (Coresponding Author); Balmages, Ilya; Reinis, Aigars et al.
19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics. NBC 2023.: Proceedings of NBC 2023. ed. / Yuri Dekhtyar; Inga Saknite. Vol. 89 Springer, 2023. p. 313 -319 (IFMBE Proceedings; Vol. 89).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Dynamic Laser Speckle Imaging for Fast Evaluation of the Antibacterial Susceptibility by the Disc Diffusion Method

AU - Lihacova, Ilze

AU - Balmages, Ilya

AU - Reinis, Aigars

AU - Kistkins, Svjatoslavs

AU - Bliznuks, Dmitrijs

AU - Plorina, Emilija V.

AU - Lihachev, Alexey

PY - 2023/7/16

Y1 - 2023/7/16

N2 - Phenotypic resistance tests e.g., disc diffusion method require 16–24 h to obtain the results, while the PCR tests provide only genotypic type of antibacterial resistance. New methods are needed to assess antibacterial resistance faster than existing methods, thus providing targeted pharmacological intervention at the early stage of the disease, increasing a patient's survival chances. Laser speckle imaging technique allows tracking moving particles in optically inhomogeneous media, allowing to observe the processes of changing the behavior of the sterile zone. The choice and adaptation of a mathematical model describing changes in the growth diameter of the sterile zone as a function of time and comparison with experimental data will help to understand and describe the formation of the sterile zone. The aim is to create processing algorithms for the obtained images to predict the diameter at the end of the disc diffusion test from the diameter of the earliest visible sterile zone.

AB - Phenotypic resistance tests e.g., disc diffusion method require 16–24 h to obtain the results, while the PCR tests provide only genotypic type of antibacterial resistance. New methods are needed to assess antibacterial resistance faster than existing methods, thus providing targeted pharmacological intervention at the early stage of the disease, increasing a patient's survival chances. Laser speckle imaging technique allows tracking moving particles in optically inhomogeneous media, allowing to observe the processes of changing the behavior of the sterile zone. The choice and adaptation of a mathematical model describing changes in the growth diameter of the sterile zone as a function of time and comparison with experimental data will help to understand and describe the formation of the sterile zone. The aim is to create processing algorithms for the obtained images to predict the diameter at the end of the disc diffusion test from the diameter of the earliest visible sterile zone.

U2 - 10.1007/978-3-031-37132-5_39

DO - 10.1007/978-3-031-37132-5_39

M3 - Chapter

SN - 978-3-031-37131-8

VL - 89

T3 - IFMBE Proceedings

SP - 313

EP - 319

BT - 19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics. NBC 2023.

A2 - Dekhtyar, Yuri

A2 - Saknite, Inga

PB - Springer

T2 - 19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics

Y2 - 12 June 2023 through 14 June 2023

ER -

Lihacova I, Balmages I, Reinis A, Kistkins S, Bliznuks D, Plorina EV et al. Dynamic Laser Speckle Imaging for Fast Evaluation of the Antibacterial Susceptibility by the Disc Diffusion Method. In Dekhtyar Y, Saknite I, editors, 19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics. NBC 2023.: Proceedings of NBC 2023. Vol. 89. Springer. 2023. p. 313 -319. (IFMBE Proceedings). doi: 10.1007/978-3-031-37132-5_39

Dynamic Laser Speckle Imaging for Fast Evaluation of the Antibacterial Susceptibility by the Disc Diffusion Method

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