Effect of Bacteriophage-Derived Double Stranded RNA on Rat Peritoneal Macrophages and Microglia in Normoxia and Hypoxia

Dace Pjanova; Yevheniia Hurmach; Mariia Rudyk; Natalia Khranovska; Oksana Skachkova; Irina Verhovcova; Larysa Skivka

doi:10.2478/prolas-2021-0050

Effect of Bacteriophage-Derived Double Stranded RNA on Rat Peritoneal Macrophages and Microglia in Normoxia and Hypoxia

Dace Pjanova (Corresponding Author), Yevheniia Hurmach, Mariia Rudyk, Natalia Khranovska, Oksana Skachkova, Irina Verhovcova, Larysa Skivka

Research output: Contribution to journal › Article › peer-review

Abstract

The involvement of tissue-resident macrophages (TRMs) in health and diseases makes them unique therapeutic targets. TRMs are activated through their surface pattern recognition receptors, such as Toll-like receptors (TLRs) that are essential sensors of danger signals. Here, we determine the activation status of rat peritoneal macrophages (PMs) and microglia (MG) cells under normal and hypoxic conditions and investigate the effect of TLR3 agonist bacteriophage-derived dsRNA (Larifan) on the metabolic profile of TRMs in vitro. We implemented the phenotypic markers CD14 and CD206, arginine metabolism, phagocytic activity and reactive oxygen species generation as metabolic characteristics to evaluate TRMs activation. We showed that normoxic TRMs from different tissue niches responded to Larifan exposure in different ways. PM exhibited signs towards M1 polarisation. In contrast, the MG activation pattern could be considered as neither pro-inflammatory nor anti-inflammatory. We also showed that TRMs, regardless of the tissue niche, responded to hypoxia with a phenotypic shift towards an anti-inflammatory (M2) state. Larifan could attenuate hypoxia-induced TRMs metabolic programming. However, hypoxic conditions could negatively affect the interaction of TRMs with danger signals.

Original language	English
Pages (from-to)	343-349
Number of pages	7
Journal	Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences
Volume	75
Issue number	5
DOIs	https://doi.org/10.2478/prolas-2021-0050
Publication status	Published - 1 Oct 2021
Externally published	Yes

Keywords*

Larifan
phenotypic shift
tissue-resident macrophages
TLR3 agonists

Field of Science*

1.6 Biological sciences
3.1 Basic medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.2478/prolas-2021-0050

Cite this

Pjanova, D., Hurmach, Y., Rudyk, M., Khranovska, N., Skachkova, O., Verhovcova, I., & Skivka, L. (2021). Effect of Bacteriophage-Derived Double Stranded RNA on Rat Peritoneal Macrophages and Microglia in Normoxia and Hypoxia. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 75(5), 343-349. https://doi.org/10.2478/prolas-2021-0050

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title = "Effect of Bacteriophage-Derived Double Stranded RNA on Rat Peritoneal Macrophages and Microglia in Normoxia and Hypoxia",

abstract = "The involvement of tissue-resident macrophages (TRMs) in health and diseases makes them unique therapeutic targets. TRMs are activated through their surface pattern recognition receptors, such as Toll-like receptors (TLRs) that are essential sensors of danger signals. Here, we determine the activation status of rat peritoneal macrophages (PMs) and microglia (MG) cells under normal and hypoxic conditions and investigate the effect of TLR3 agonist bacteriophage-derived dsRNA (Larifan) on the metabolic profile of TRMs in vitro. We implemented the phenotypic markers CD14 and CD206, arginine metabolism, phagocytic activity and reactive oxygen species generation as metabolic characteristics to evaluate TRMs activation. We showed that normoxic TRMs from different tissue niches responded to Larifan exposure in different ways. PM exhibited signs towards M1 polarisation. In contrast, the MG activation pattern could be considered as neither pro-inflammatory nor anti-inflammatory. We also showed that TRMs, regardless of the tissue niche, responded to hypoxia with a phenotypic shift towards an anti-inflammatory (M2) state. Larifan could attenuate hypoxia-induced TRMs metabolic programming. However, hypoxic conditions could negatively affect the interaction of TRMs with danger signals.",

keywords = "Larifan, phenotypic shift, tissue-resident macrophages, TLR3 agonists",

author = "Dace Pjanova and Yevheniia Hurmach and Mariia Rudyk and Natalia Khranovska and Oksana Skachkova and Irina Verhovcova and Larysa Skivka",

note = "Publisher Copyright: {\textcopyright} 2021 Dace Pjanova et al., published by Sciendo.",

year = "2021",

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day = "1",

doi = "10.2478/prolas-2021-0050",

language = "English",

volume = "75",

pages = "343--349",

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Pjanova, D, Hurmach, Y, Rudyk, M, Khranovska, N, Skachkova, O, Verhovcova, I & Skivka, L 2021, 'Effect of Bacteriophage-Derived Double Stranded RNA on Rat Peritoneal Macrophages and Microglia in Normoxia and Hypoxia', Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, vol. 75, no. 5, pp. 343-349. https://doi.org/10.2478/prolas-2021-0050

Effect of Bacteriophage-Derived Double Stranded RNA on Rat Peritoneal Macrophages and Microglia in Normoxia and Hypoxia. / Pjanova, Dace (Corresponding Author); Hurmach, Yevheniia; Rudyk, Mariia et al.
In: Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, Vol. 75, No. 5, 01.10.2021, p. 343-349.

Research output: Contribution to journal › Article › peer-review

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T1 - Effect of Bacteriophage-Derived Double Stranded RNA on Rat Peritoneal Macrophages and Microglia in Normoxia and Hypoxia

AU - Pjanova, Dace

AU - Hurmach, Yevheniia

AU - Rudyk, Mariia

AU - Khranovska, Natalia

AU - Skachkova, Oksana

AU - Verhovcova, Irina

AU - Skivka, Larysa

PY - 2021/10/1

Y1 - 2021/10/1

N2 - The involvement of tissue-resident macrophages (TRMs) in health and diseases makes them unique therapeutic targets. TRMs are activated through their surface pattern recognition receptors, such as Toll-like receptors (TLRs) that are essential sensors of danger signals. Here, we determine the activation status of rat peritoneal macrophages (PMs) and microglia (MG) cells under normal and hypoxic conditions and investigate the effect of TLR3 agonist bacteriophage-derived dsRNA (Larifan) on the metabolic profile of TRMs in vitro. We implemented the phenotypic markers CD14 and CD206, arginine metabolism, phagocytic activity and reactive oxygen species generation as metabolic characteristics to evaluate TRMs activation. We showed that normoxic TRMs from different tissue niches responded to Larifan exposure in different ways. PM exhibited signs towards M1 polarisation. In contrast, the MG activation pattern could be considered as neither pro-inflammatory nor anti-inflammatory. We also showed that TRMs, regardless of the tissue niche, responded to hypoxia with a phenotypic shift towards an anti-inflammatory (M2) state. Larifan could attenuate hypoxia-induced TRMs metabolic programming. However, hypoxic conditions could negatively affect the interaction of TRMs with danger signals.

AB - The involvement of tissue-resident macrophages (TRMs) in health and diseases makes them unique therapeutic targets. TRMs are activated through their surface pattern recognition receptors, such as Toll-like receptors (TLRs) that are essential sensors of danger signals. Here, we determine the activation status of rat peritoneal macrophages (PMs) and microglia (MG) cells under normal and hypoxic conditions and investigate the effect of TLR3 agonist bacteriophage-derived dsRNA (Larifan) on the metabolic profile of TRMs in vitro. We implemented the phenotypic markers CD14 and CD206, arginine metabolism, phagocytic activity and reactive oxygen species generation as metabolic characteristics to evaluate TRMs activation. We showed that normoxic TRMs from different tissue niches responded to Larifan exposure in different ways. PM exhibited signs towards M1 polarisation. In contrast, the MG activation pattern could be considered as neither pro-inflammatory nor anti-inflammatory. We also showed that TRMs, regardless of the tissue niche, responded to hypoxia with a phenotypic shift towards an anti-inflammatory (M2) state. Larifan could attenuate hypoxia-induced TRMs metabolic programming. However, hypoxic conditions could negatively affect the interaction of TRMs with danger signals.

KW - Larifan

KW - phenotypic shift

KW - tissue-resident macrophages

KW - TLR3 agonists

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U2 - 10.2478/prolas-2021-0050

DO - 10.2478/prolas-2021-0050

M3 - Article

AN - SCOPUS:85139182818

SN - 1407-009X

VL - 75

SP - 343

EP - 349

JO - Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences

JF - Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences

IS - 5

ER -

Effect of Bacteriophage-Derived Double Stranded RNA on Rat Peritoneal Macrophages and Microglia in Normoxia and Hypoxia

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