Effect of cultivation practice on the phytochemical profile and biological activity of aqueous ethanol extracts of Chelidonium majus L.

Inga Sīle, Valerija Krizhanovska, Arta Kronberga, Ilva Nakurte, Ieva Mezaka, Maija Dambrova, Osvalds Pugovičs, Solveiga Grinberga

Research output: Contribution to conferencePoster

Abstract

Chelidonium majus is a valuable medicinal plant traditionally used to treat gastric, liver, and skin diseases. The aim of this study was to investigate how cultivation affects both the phytochemical composition and the cytotoxic activity of aqueous ethanol extracts of C. majus. The extracts were prepared from aerial parts of wild-grown and cultivated specimens. LC/MS methods were used to analyze the qualitative and quantitative composition of alkaloids and flavonoid derivatives. The cytotoxic activities of the C. majus extracts were determined in B16-F10, HepG2, and CaCo-2 cells. The total content of alkaloids in extracts prepared from cultivated C. majus was significantly higher than that of wild samples. Coptisine was the dominant compound in wild- grown C. majus extracts, however, chelidonine was found to be the dominant alkaloid after cultivation. C. majus cultivation did not significantly affect the total content of flavonol glycosides, however, the content of both rutin and isorhamnetin 3-O-rutinoside showed clear tendencies of increase. The extracts prepared from cultivated specimens showed higher cytotoxicity than the extracts from wild-grown plants. HepG2 and CaCo-2 cells were less sensitive than melanoma cells. The observed differences in the phytochemical composition of the C. majus extracts resulted in a significant increase in cytotoxic activity.
Original languageEnglish
Number of pages5
Publication statusPublished - 2021
Event8th European Virtual Congress of Pharmacology (EPHAR 2021) - Virtual
Duration: 6 Dec 20218 Dec 2021
https://www.ephar2021.org/

Congress

Congress8th European Virtual Congress of Pharmacology (EPHAR 2021)
Period6/12/218/12/21
Internet address

Field of Science*

  • 3.1 Basic medicine

Publication Type*

  • 6. Other publications

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