Establishing Reference Intervals for von Willebrand Factor Multimers

M. Pikta, M. Vasse, Sandra Lejniece, K.J. Smock, K.A. Mosser, H. Bautista, G. Nouadje, V. Banys

Research output: Contribution to journalMeeting Abstractpeer-review


Background: von Willebrand factor multimers (VWF:MM) methodologies are technically difficult, laborious, time consuming, non-standardized and results vary between labs. A first step towards standardization was made by introducing a semi‐automated VWF:MM assay by Sebia (Lisses, France). In addition to qualitative interpretation of multimer patterns, the Sebia PHORESIS software allows quantification of VWF:MM band patterns, thus the percentage of each molecular weight multimer fraction can be calculated. Due to lack of reference values for VWF:MM fractions, results interpretation can be challenging in some cases.

Aims: The aim of this study was to determine reference intervals for low molecular weight (LMW), intermediate molecular weight (IMW) and high molecular weight (HMW) multimers.

Methods: To collect a larger sample size an international cooperation was initiated. We analyzed data from 134 participants (51 males and 83 non-pregnant females) aged 17-69 years. Acceptance criteria: no history of hemorrhagic episodes; no usage of any medication for at least 10 days before blood collection; normal coagulation screening tests; normal VWF screening assay results; written consent provided.
Reference intervals (with 90% confidence intervals) were calculated using a robust method according to the CLSI C28-A3 standard. The study was performed according to the Declaration of Helsinki and was approved by national ethical committees.

Results: After outlier exclusion (n=3), done by Grubs double sided and Tukey methods, a total of 131 samples were analyzed statistically. Results were normally distributed. No significant differences were found between males and females. The proposed reference intervals for VWF:MM are presented in table 1.

Conclusions: Quantification of VWF:MM fractions, in addition to qualitative assessment of VWF:MM patterns, has potential value to aid in differential diagnosis of VWD sub-types. The reference values calculated in this study can be used in future research to establish clinical decision liimits.
Original languageEnglish
Article numberPB1574
Number of pages2
JournalResearch and Practice in Thrombosis and Haemostasis
Issue numberS1
Publication statusPublished - Aug 2020
EventCongress of the International Society on Thrombosis and Haemostasis (ISTH) - virtual
Duration: 12 Jul 202014 Jul 2020

Field of Science*

  • 3.2 Clinical medicine

Publication Type*

  • 3.4. Other publications in conference proceedings (including local)


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