Evaluation of a new semi-automated hydragel 11 von willebrand factor multimers assay kit for routine use

Marika Pikta (Coresponding Author), Timea Szanto, Margus Viigimaa, Sandra Lejniece, Darta Balode, Kadri Saks, Valdas Banys

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Background: Accurate diagnosis and classification of von Willebrand disease (VWD) are essential for optimal management. The von Willebrand factor multimers analysis (VWF:MM) is an integral part of the diagnostic process in the phenotypic classification, especially in discrepant cases. The aim of this study was to evaluate the performance of a new Hydragel 11VWF multimer assay (H11VW). Methods: Analytical performance characteristics such as repeatability (intra-assay variability, in gel between track variation), reproducibility (inter-assay variability, between gel variation), sensitivity, EQA performance and differences between two commercially available VWF:MM kits (H5VW and H11VW) were analysed in healthy volunteers' plasmas using in-house prepared reference plasma. Results: Repeatability and reproducibility results of H11VW demonstrated acceptable and equivalent performance with previously verified H5VW. Participation in EQA was successful. No statistically significant difference was detected between H5VW and H11VW kits for different fractions of multimers: LMWM p=0.807; IMWM p=0.183; HMWM p=0.774. Conclusions: H11VW demonstrated acceptable analytical performance characteristics. H11VW kit conveniently offers a more significant number of samples on a single gel. H5VW and H11VW kits can be used in daily practice interchangeably.

Original languageEnglish
Pages (from-to)167-172
Number of pages5
JournalJournal of Medical Biochemistry
Volume40
Issue number2
DOIs
Publication statusPublished - 2021

Keywords*

  • Electrophoresis
  • Multimers
  • Von Willebrand disease
  • Von Willebrand factor

Field of Science*

  • 3.4 Medical biotechnology
  • 1.6 Biological sciences

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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