strategies for acute uncomplicated appendicitis has evolved that
conservative antibacterial treatment is now recommended over surgical
treatment, especially in pediatric patients. The aim of this
research was to evaluate microbiota in pediatric patients surgically
treated with acute uncomplicated and complicated appendicitis, and
antibacterial susceptibility of the causative microorganisms.
prospective cohort study included children with suspected AA (7-17 year
age). Bacteriological examination was performed on swab samples from
the distal and proximal ends of the appendiceal lumen, submucosa of
the appendix, and peritoneal cavity. Bacteriological identification
was realized using the VITEK2 analyser. Antibacterial susceptibility
tests were performed in accordance with the recommendations of the
European Committee on Antimicrobial Susceptibility Testing (EUCAST).
Serodiagnosis of Yersenia
was performed using indirect haemagglutination. Microsoft Excel 2016
and IBM SPSS Statistics 22 were used for statistical analysis. This
study was performed in accordance with the ethical standards of the
results revealed differences in microbiota in cases of acute
complicated and acute uncomplicated appendicitis. Pseudomonas
was identified more frequently in cases of acute complicated
appendicitis. Mixed culutre was prevalent in cases of acute
complicated and acute uncomplicated appendicitis. Only a couple
positive extended spectrum beta-lactamase ( ESBL ) E.
cultures were identified.Most of the strains of Pseudomonas
were resitant to amoxicillin and clavulanic acid, ertapenem,
ampicillin and cefotaxime. E.
isolates were resistent to ampicillin and to amoxicillin with
cases of acute complicated appendicitis, P.
is the prevalent microorganism, whereas E.
is the most commonly isolated microorganism in acute uncomplicated
appendicitis. Treatment strategies for AA should include antibiotics
with different mechanisms of action to achieve a synergistic effect
and prevent the development of antibiotic resistance. Guidelines for
empiric antibiotic therapy should be reviewed periodically to ensure
compliance with current antibacterial susceptibility patterns.
- 3.4. Other publications in conference proceedings (including local)