TY - JOUR
T1 - Evaluation of the physical and antimicrobial properties of silver doped hydroxyapatite depending on the preparation method
AU - Dubnika, Arita
AU - Loca, Dagnija
AU - Salma, Ilze
AU - Reinis, Aigars
AU - Poca, Lasma
AU - Berzina-Cimdina, Liga
N1 - Funding Information:
Acknowledgments This work was partially supported by thr Riga Technical University within the Project No. ZP-2012/24 and the European Social Fund within the project ‘‘Support for the implementation of doctoral studies at Riga Technical University’’.
PY - 2014/2
Y1 - 2014/2
N2 - In the present study, the effect of the preparation method on the physical and antibacterial properties of silver doped hydroxyapatite (HAp/Ag) samples was investigated. HAp/Ag with 0.1-5 % of silver was prepared using two different modified wet chemical precipitation methods. A comparison of thermal stability and thermodynamical properties indicated that the thermal stability and sintering temperature of HAp/Ag were higher than those of pure hydroxyapatite if Ca(NO3)2·4H2O, AgNO3, NH4OH and (NH4)2HPO4 were used as raw materials. Phase composition and silver release were determined by XRD and ICP-MS. The study showed that, after 50 h in simulated body fluid 0.8-1.8 % of silver of the total silver amount was released from compact HAp/Ag scaffolds, and release kinetics strongly depended on the HAp/Ag preparation method. In vitro antibacterial activity of samples from each method against the bacterial strains Staphylococcus epidermidis and Pseudomonas aeruginosa was approved. Results showed that, in the case of using Ca(OH)2, H 3PO4 and AgNO3 as raw materials for HAp/Ag synthesis, higher antibacterial activity towards both bacterial strains could be obtained.
AB - In the present study, the effect of the preparation method on the physical and antibacterial properties of silver doped hydroxyapatite (HAp/Ag) samples was investigated. HAp/Ag with 0.1-5 % of silver was prepared using two different modified wet chemical precipitation methods. A comparison of thermal stability and thermodynamical properties indicated that the thermal stability and sintering temperature of HAp/Ag were higher than those of pure hydroxyapatite if Ca(NO3)2·4H2O, AgNO3, NH4OH and (NH4)2HPO4 were used as raw materials. Phase composition and silver release were determined by XRD and ICP-MS. The study showed that, after 50 h in simulated body fluid 0.8-1.8 % of silver of the total silver amount was released from compact HAp/Ag scaffolds, and release kinetics strongly depended on the HAp/Ag preparation method. In vitro antibacterial activity of samples from each method against the bacterial strains Staphylococcus epidermidis and Pseudomonas aeruginosa was approved. Results showed that, in the case of using Ca(OH)2, H 3PO4 and AgNO3 as raw materials for HAp/Ag synthesis, higher antibacterial activity towards both bacterial strains could be obtained.
UR - http://www.scopus.com/inward/record.url?scp=84894493336&partnerID=8YFLogxK
U2 - 10.1007/s10856-013-5079-y
DO - 10.1007/s10856-013-5079-y
M3 - Article
C2 - 24170340
AN - SCOPUS:84894493336
SN - 0957-4530
VL - 25
SP - 435
EP - 444
JO - Journal of Materials Science: Materials in Medicine
JF - Journal of Materials Science: Materials in Medicine
IS - 2
ER -