Examination of in vitro and in vivo biocompatibility of alginate-hyaluronic acid microbeads as a promising method in cell delivery for kidney regeneration

Jhaleh Amirian; Tran Thi Tuong Van; Sang Ho Bae; Hae Il Jung; Hwan Jun Choi; Hyun Deuk Cho; Byong Taek Lee

doi:10.1016/j.ijbiomac.2017.07.019

Examination of in vitro and in vivo biocompatibility of alginate-hyaluronic acid microbeads as a promising method in cell delivery for kidney regeneration

Jhaleh Amirian, Tran Thi Tuong Van, Sang Ho Bae, Hae Il Jung, Hwan Jun Choi, Hyun Deuk Cho, Byong Taek Lee (Corresponding Author)

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

In this study, alginate (ALG) and alginate-hyaluronic acid (ALG-HA) injectable microbeads, with the purpose of delivering stem cells for tissue engineering, were prepared by a spraying method into a CaCl₂ solution that shows high porosity for the exchange of nutrition and waste. In addition, the size distribution and surface morphology was investigated using optical and scanning electron microscopy, respectively. The chemical structural properties of the ALG-HA microbeads were examined by Fourier transform infrared spectroscopy. The biocompatibility of ALG and ALG-HA microbeads was examined in vitro. Rat bone marrow stem cells were encapsulated in microbeads to investigate cell release, cell viability, proliferation, and secretion of growth factors such as VEGF and PDGF. Growth factors were released for the 21 day experimental period. Cells were found to be released from the microbeads after 7 days. Furthermore, the in vivo biocompatibility of ALG-HA microbeads was examined using microbeads without cell encapsulation in the kidney capsule, in order to assess the foreign body reaction and inflammatory response, for 14 days. The desired in vivo response to ALG-HA microbeads hydrogel makes it an exquisite candidate for subcapsular cell and drug delivery to kidney tissue.

Original language	English
Pages (from-to)	143-153
Number of pages	11
Journal	International Journal of Biological Macromolecules
Volume	105
Issue number	Part 1
DOIs	https://doi.org/10.1016/j.ijbiomac.2017.07.019
Publication status	Published - Dec 2017
Externally published	Yes

Keywords*

ALG-HA microbeads
Encapsulation
In vivo biocompatibility
Kidney
RBMSCs

Field of Science*

1.4 Chemical sciences
2.5 Materials engineering
2.6 Medical engineering
3.1 Basic medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1016/j.ijbiomac.2017.07.019

Cite this

Amirian, J., Van, T. T. T., Bae, S. H., Jung, H. I., Choi, H. J., Cho, H. D., & Lee, B. T. (2017). Examination of in vitro and in vivo biocompatibility of alginate-hyaluronic acid microbeads as a promising method in cell delivery for kidney regeneration. International Journal of Biological Macromolecules, 105(Part 1), 143-153. https://doi.org/10.1016/j.ijbiomac.2017.07.019

@article{d93137ea85c841018aa1329fcfb0a01e,

title = "Examination of in vitro and in vivo biocompatibility of alginate-hyaluronic acid microbeads as a promising method in cell delivery for kidney regeneration",

abstract = "In this study, alginate (ALG) and alginate-hyaluronic acid (ALG-HA) injectable microbeads, with the purpose of delivering stem cells for tissue engineering, were prepared by a spraying method into a CaCl2 solution that shows high porosity for the exchange of nutrition and waste. In addition, the size distribution and surface morphology was investigated using optical and scanning electron microscopy, respectively. The chemical structural properties of the ALG-HA microbeads were examined by Fourier transform infrared spectroscopy. The biocompatibility of ALG and ALG-HA microbeads was examined in vitro. Rat bone marrow stem cells were encapsulated in microbeads to investigate cell release, cell viability, proliferation, and secretion of growth factors such as VEGF and PDGF. Growth factors were released for the 21 day experimental period. Cells were found to be released from the microbeads after 7 days. Furthermore, the in vivo biocompatibility of ALG-HA microbeads was examined using microbeads without cell encapsulation in the kidney capsule, in order to assess the foreign body reaction and inflammatory response, for 14 days. The desired in vivo response to ALG-HA microbeads hydrogel makes it an exquisite candidate for subcapsular cell and drug delivery to kidney tissue.",

keywords = "ALG-HA microbeads, Encapsulation, In vivo biocompatibility, Kidney, RBMSCs",

author = "Jhaleh Amirian and Van, {Tran Thi Tuong} and Bae, {Sang Ho} and Jung, {Hae Il} and Choi, {Hwan Jun} and Cho, {Hyun Deuk} and Lee, {Byong Taek}",

note = "Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and future Planning (No. 2015R1A2A1A10053272 ) and the Ministry of Education ( 2015R1A6A1A03032522 ). Furthermore, this research was and partially supported by SoonChunHyang University research fund ( 20150000 ). All project funded by South Korea government. The authors are grateful to Mr Paul Kallyanashisfor providing guidelines and suggestions in the design of the spraying machine. Publisher Copyright: {\textcopyright} 2017",

year = "2017",

month = dec,

doi = "10.1016/j.ijbiomac.2017.07.019",

language = "English",

volume = "105",

pages = "143--153",

journal = "International Journal of Biological Macromolecules",

issn = "0141-8130",

publisher = "Elsevier B.V.",

number = "Part 1",

}

Examination of in vitro and in vivo biocompatibility of alginate-hyaluronic acid microbeads as a promising method in cell delivery for kidney regeneration. / Amirian, Jhaleh; Van, Tran Thi Tuong; Bae, Sang Ho et al.
In: International Journal of Biological Macromolecules, Vol. 105, No. Part 1, 12.2017, p. 143-153.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Examination of in vitro and in vivo biocompatibility of alginate-hyaluronic acid microbeads as a promising method in cell delivery for kidney regeneration

AU - Amirian, Jhaleh

AU - Van, Tran Thi Tuong

AU - Bae, Sang Ho

AU - Jung, Hae Il

AU - Choi, Hwan Jun

AU - Cho, Hyun Deuk

AU - Lee, Byong Taek

N1 - Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and future Planning (No. 2015R1A2A1A10053272 ) and the Ministry of Education ( 2015R1A6A1A03032522 ). Furthermore, this research was and partially supported by SoonChunHyang University research fund ( 20150000 ). All project funded by South Korea government. The authors are grateful to Mr Paul Kallyanashisfor providing guidelines and suggestions in the design of the spraying machine. Publisher Copyright: © 2017

PY - 2017/12

Y1 - 2017/12

N2 - In this study, alginate (ALG) and alginate-hyaluronic acid (ALG-HA) injectable microbeads, with the purpose of delivering stem cells for tissue engineering, were prepared by a spraying method into a CaCl2 solution that shows high porosity for the exchange of nutrition and waste. In addition, the size distribution and surface morphology was investigated using optical and scanning electron microscopy, respectively. The chemical structural properties of the ALG-HA microbeads were examined by Fourier transform infrared spectroscopy. The biocompatibility of ALG and ALG-HA microbeads was examined in vitro. Rat bone marrow stem cells were encapsulated in microbeads to investigate cell release, cell viability, proliferation, and secretion of growth factors such as VEGF and PDGF. Growth factors were released for the 21 day experimental period. Cells were found to be released from the microbeads after 7 days. Furthermore, the in vivo biocompatibility of ALG-HA microbeads was examined using microbeads without cell encapsulation in the kidney capsule, in order to assess the foreign body reaction and inflammatory response, for 14 days. The desired in vivo response to ALG-HA microbeads hydrogel makes it an exquisite candidate for subcapsular cell and drug delivery to kidney tissue.

AB - In this study, alginate (ALG) and alginate-hyaluronic acid (ALG-HA) injectable microbeads, with the purpose of delivering stem cells for tissue engineering, were prepared by a spraying method into a CaCl2 solution that shows high porosity for the exchange of nutrition and waste. In addition, the size distribution and surface morphology was investigated using optical and scanning electron microscopy, respectively. The chemical structural properties of the ALG-HA microbeads were examined by Fourier transform infrared spectroscopy. The biocompatibility of ALG and ALG-HA microbeads was examined in vitro. Rat bone marrow stem cells were encapsulated in microbeads to investigate cell release, cell viability, proliferation, and secretion of growth factors such as VEGF and PDGF. Growth factors were released for the 21 day experimental period. Cells were found to be released from the microbeads after 7 days. Furthermore, the in vivo biocompatibility of ALG-HA microbeads was examined using microbeads without cell encapsulation in the kidney capsule, in order to assess the foreign body reaction and inflammatory response, for 14 days. The desired in vivo response to ALG-HA microbeads hydrogel makes it an exquisite candidate for subcapsular cell and drug delivery to kidney tissue.

KW - ALG-HA microbeads

KW - Encapsulation

KW - In vivo biocompatibility

KW - Kidney

KW - RBMSCs

UR - http://www.scopus.com/inward/record.url?scp=85023619593&partnerID=8YFLogxK

UR - https://www-sciencedirect-com.db.rsu.lv/science/article/pii/S0141813016327763?via%3Dihub

U2 - 10.1016/j.ijbiomac.2017.07.019

DO - 10.1016/j.ijbiomac.2017.07.019

M3 - Article

C2 - 28698077

AN - SCOPUS:85023619593

SN - 0141-8130

VL - 105

SP - 143

EP - 153

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

IS - Part 1

ER -

Examination of in vitro and in vivo biocompatibility of alginate-hyaluronic acid microbeads as a promising method in cell delivery for kidney regeneration

Abstract

Keywords*

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