TY - JOUR
T1 - Examination of in vitro and in vivo biocompatibility of alginate-hyaluronic acid microbeads as a promising method in cell delivery for kidney regeneration
AU - Amirian, Jhaleh
AU - Van, Tran Thi Tuong
AU - Bae, Sang Ho
AU - Jung, Hae Il
AU - Choi, Hwan Jun
AU - Cho, Hyun Deuk
AU - Lee, Byong Taek
N1 - Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and future Planning (No. 2015R1A2A1A10053272 ) and the Ministry of Education ( 2015R1A6A1A03032522 ). Furthermore, this research was and partially supported by SoonChunHyang University research fund ( 20150000 ). All project funded by South Korea government. The authors are grateful to Mr Paul Kallyanashisfor providing guidelines and suggestions in the design of the spraying machine.
Publisher Copyright:
© 2017
PY - 2017/12
Y1 - 2017/12
N2 - In this study, alginate (ALG) and alginate-hyaluronic acid (ALG-HA) injectable microbeads, with the purpose of delivering stem cells for tissue engineering, were prepared by a spraying method into a CaCl2 solution that shows high porosity for the exchange of nutrition and waste. In addition, the size distribution and surface morphology was investigated using optical and scanning electron microscopy, respectively. The chemical structural properties of the ALG-HA microbeads were examined by Fourier transform infrared spectroscopy. The biocompatibility of ALG and ALG-HA microbeads was examined in vitro. Rat bone marrow stem cells were encapsulated in microbeads to investigate cell release, cell viability, proliferation, and secretion of growth factors such as VEGF and PDGF. Growth factors were released for the 21 day experimental period. Cells were found to be released from the microbeads after 7 days. Furthermore, the in vivo biocompatibility of ALG-HA microbeads was examined using microbeads without cell encapsulation in the kidney capsule, in order to assess the foreign body reaction and inflammatory response, for 14 days. The desired in vivo response to ALG-HA microbeads hydrogel makes it an exquisite candidate for subcapsular cell and drug delivery to kidney tissue.
AB - In this study, alginate (ALG) and alginate-hyaluronic acid (ALG-HA) injectable microbeads, with the purpose of delivering stem cells for tissue engineering, were prepared by a spraying method into a CaCl2 solution that shows high porosity for the exchange of nutrition and waste. In addition, the size distribution and surface morphology was investigated using optical and scanning electron microscopy, respectively. The chemical structural properties of the ALG-HA microbeads were examined by Fourier transform infrared spectroscopy. The biocompatibility of ALG and ALG-HA microbeads was examined in vitro. Rat bone marrow stem cells were encapsulated in microbeads to investigate cell release, cell viability, proliferation, and secretion of growth factors such as VEGF and PDGF. Growth factors were released for the 21 day experimental period. Cells were found to be released from the microbeads after 7 days. Furthermore, the in vivo biocompatibility of ALG-HA microbeads was examined using microbeads without cell encapsulation in the kidney capsule, in order to assess the foreign body reaction and inflammatory response, for 14 days. The desired in vivo response to ALG-HA microbeads hydrogel makes it an exquisite candidate for subcapsular cell and drug delivery to kidney tissue.
KW - ALG-HA microbeads
KW - Encapsulation
KW - In vivo biocompatibility
KW - Kidney
KW - RBMSCs
UR - http://www.scopus.com/inward/record.url?scp=85023619593&partnerID=8YFLogxK
UR - https://www-sciencedirect-com.db.rsu.lv/science/article/pii/S0141813016327763?via%3Dihub
U2 - 10.1016/j.ijbiomac.2017.07.019
DO - 10.1016/j.ijbiomac.2017.07.019
M3 - Article
C2 - 28698077
AN - SCOPUS:85023619593
SN - 0141-8130
VL - 105
SP - 143
EP - 153
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
IS - Part 1
ER -