The present work deals with the altered expression of cytokeratins (CK) in stratified oral epithelium in relation to changes at the cellular differentiation level. Several monoclonal antibodies to basic and acidic cytokeratins (AE1, AE3, CK10, CK10/13, CK5/6, CK17, CK18, CK19) were used immunohistochemically to study the intensity and quantity of cytokeratin expression in normal, hyperplastic oral epithelium, as well as dysplastic lesions and squamous cell carcinoma. No reaction was found by applying monoclonal antibodies to CK18 and CK19. The main results of our study were: (1) the expression of acidic, low molecular weight cytokeratins AE1, and changes in differentiation specific markers CK10 and CK10/13 with the dedifferentiation of epithelial cells, (2) no observed reactions of CK10 and CK10/13 in squamous cell carcinoma, (3) increase of expression of CK5/6 with the reduction of differentiation of epithelial cells. The presented findings confirm the high sensitivity and specificity of the applied monoclonal cytokeratin antibodies to indicate the degree of differentiation in oral dysplasias and carcinomas.
|Journal||Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.|
|Publication status||Published - 1998|
- epithelial differentiation
- oral mucosa
Field of Science*
- 3.2 Clinical medicine
- 1.1. Scientific article indexed in Web of Science and/or Scopus database