Expression of ORAI1 and STIM1 genes in blood of patients with pulmonary tuberculosis

Oksana Kolesova (Coresponding Author), Ksenija Kramica, Aleksandrs Kolesovs, Jelena Eglite

Research output: Contribution to journalArticlepeer-review

Abstract

This study aimed to detect the expression level of ORAI1 and STIM1 genes in blood of patients with bilateral pulmonary tuberculosis (TB) in comparison with the control group. Both genes encode proteins providing store-operated Ca2+ entry (SOCE) into the cells, including immune cells, to activate transcriptional factors for producing cytokines and inflammation-restricting proteins. The study included 45 patients with confirmed TB, aged 20 to 86, and 35 volunteers, aged from 21 to 73, without active TB infection. The expression of ORAI1 and STIM1 genes in blood was performed by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the referent gene. Inflammation was assessed by levels of interferon γ (IFN-γ) and interleukin 18 (IL-18) in serum (ELISA method). The results showed lower expression of ORAI1 in blood and higher levels of IFN-γ and IL-18 in serum of TB patients than that of the control group and no differences in expression of the STIM1 gene. It indicates some impairment in the SOCE mechanism of immune cells, which is associated with TB.

Original languageEnglish
Pages (from-to)275-282
Number of pages8
JournalCentral European Journal of Immunology
Volume46
Issue number2
DOIs
Publication statusPublished - 2021

Keywords*

  • Cytokines
  • MRNA ORAI1
  • MRNA STIM1
  • SOCE
  • Tuberculosis

Field of Science*

  • 1.6 Biological sciences

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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