Fibronectin-binding nanoparticles for intracellular targeting addressed by B. burgdorferi BBK32 protein fragments

Renate Ranka, Ivars Petrovskis, Irina Sominskaya, Janis Bogans, Ruta Bruvere, Inara Akopjana, Velta Ose, Irena Timofejeva, Kalvis Brangulis, Pauls Pumpens, Viesturs Baumanis

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Virus-like particles (VLPs) are created by the self-assembly of multiple copies of envelope and/or capsid proteins from many viruses, mimicking the conformation of a native virus. Such noninfectious nanostructures are mainly used as antigen-presenting platforms, especially in vaccine research; however, some of them recently were used as scaffolds in biotechnology to produce targeted nanoparticles for intracellular delivery. This study demonstrates the creation of fusion VLPs using hepatitis B core protein-based system maintaining a fibronectin-binding property from B. burgdorferi BBK32 protein, including the evidence of particles' transmission to BHK-21 target cells via caveolae/rafts endocythosis. These results make this construct to be an attractive model in development of HBc-based nanoparticles for cellular targeting applications and highlights the fragment of B. burgdorferi BBK32 as a novel cellular uptake-promoting peptide. From the Clinical Editor: This paper discusses the nanotechnology-based application of self-assembling viral-like peptides (VLP-s) for targeted delivery using a hepatitis B core protein based system. Creating fusion VLPs may be an attractive model for cellular targeting applications.

Original languageEnglish
Pages (from-to)65-73
Number of pages9
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Issue number1
Publication statusPublished - Jan 2013
Externally publishedYes


  • B. burgdorferi
  • Fibronectin
  • Nanoparticles

Field of Science*

  • 2.5 Materials engineering
  • 2.6 Medical engineering
  • 3.1 Basic medicine

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


Dive into the research topics of 'Fibronectin-binding nanoparticles for intracellular targeting addressed by B. burgdorferi BBK32 protein fragments'. Together they form a unique fingerprint.

Cite this