Fluorescent Characteristics of Blood Leukocytes of Patients with Malignant and Nonmalignant Diseases

R. Brüvere, N. Gabruševa, I. Kalniņa, G. Feldmane, I. Meirovics

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Properties of the naphthalic acid derivative 3-isopropyloxy-6-morpholino-2-phenilphenalen-l-ona (PMPH) as a potential fluorescent probe were investigated. Spectral characteristics of the compound in different solvents as well as their binding to model lipid membranes (liposomes) and human peripheral blood mononuclear cells (lymphocytes) were determined, The fluorescence was found to be sensitive to polarity changes in the environment. Distinctions were observed in the spectral characteristics of the investigated compound when bound to liposomes and peripheral blood mononuclear cells of healthy donors, and patients with malignant and nonmalignant diseases. It was established that spectral characteristics of PMPH in cell suspension qualitatively characterize its structural and functional alterations during pathologic phenomena and correlate directly with the clinical view of disease. PMPH is shown to be a prospective method for screening for various pathologies.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalJournal of Fluorescence
Volume13
Issue number2
DOIs
Publication statusPublished - Mar 2003
Externally publishedYes

Keywords

  • Diagnostics
  • Fluorescent probe
  • Lymphocytes

Field of Science

  • 3.1 Basic medicine

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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