Fluorescent study of human blood plasma albumin alterations induced by ionizing radiation

Elena M. Kirilova, Inta Kalnina, Tija Zvagule, Natalija Gabruseva, Natalja Kurjane, Irina I. Solomenikova

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The use of hydrophobic fluorescent probe ABM (benzanthrone derivative) and albumin autofluorescence allowed show conformational alterations in Chernobyl clean-up workers blood plasma. Results obtained in 1996-1997 suggest that acidic expansion of plasma albumin takes place. Latest data (2006-2008) result in splitting of albumin alterations onto two stages - acidic expansion and N-F transition. The N-F transition is accompanied by the blue shift of fluorescence spectra and dehydration of tryptophanyl region of albumin molecule. In 2007 obtained.patterns of ABM spectra had never been previously seen in examined healthy individuals or patients with tuberculosis, multiple sclerosis, rheumatoid arthritis, etc. Patterns of ABM fluorescence spectra are associated with conformational changes of blood plasma albumin. The use of probe ABM and albumin auto-fluorescence allowed show conformational alterations in albumin of Chernobyl clean-up workers blood plasma. It is necessary to note that all investigated parameters significantly differ in observed groups of patients. These findings reinforce our understanding that the blood plasma albumin is a significant biological target of radiation. It may be concluded that fluorescence characteristics are representative of radiation induced albumin alterations and its carrier function.

Original languageEnglish
Pages (from-to)923-927
Number of pages5
JournalJournal of Fluorescence
Volume21
Issue number3
DOIs
Publication statusPublished - May 2011

Keywords

  • Human serum albumin
  • Hydrophobic fluorescent probe
  • Ionizing radiation
  • Tryptophane residue

Field of Science

  • 1.4 Chemical sciences
  • 1.6 Biological sciences
  • 5.1 Psychology
  • 5.4 Sociology
  • 5.5 Law

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

Fingerprint

Dive into the research topics of 'Fluorescent study of human blood plasma albumin alterations induced by ionizing radiation'. Together they form a unique fingerprint.

Cite this