Gallbladder interleukins in children with calculous cholecystitis

Arina Deņisova, Arnis Eņģelis, Māra Pilmane

Research output: Contribution to conferenceAbstractpeer-review


Calculous cholecystitis connects to inflammation and might be a reason for a variety of complications, such as adhesions in the abdominal cavity. Despite it being a common pathology in adults, paediatric population also displays this disease, yet it is still uncertain how inflammation factors are involved in its morphopathogenesis. Our aim was to research the distribution and appearance of different interleukins in calculous cholecystitis affected gallbladder wall in children. A total number of 20 calculous cholecystitis surgery tissue samples were obtained from 18 children, - 12 girls and 6 boys (aged 4 to 17 years, mean age 13.9 ± 3.72). As a control, 7 unaffected gallbladders were used. Tissues were immunohistochemically stained for IL-1α, IL-4, IL-6, IL-7, IL-8, IL-10, and IL-17 and the slides were inspected by light microscopy. To evaluate statistical differences and correlations between interleukins, Mann-Whitney U and Spearman’s tests were used. Statistically significant difference between patient and control gallbladder epithelium was for IL-1α and IL-17, but connective tissues – IL-1α, IL-4, IL-6, IL-7, IL-8, IL-17 positive structures. The highest value of interleukin positive cells in epithelium was for IL-17 (2.235 ± 0.589), but in connective tissues for IL-7 (2.2 ± 0.410), IL-6 (2.150 ± 0.432) and IL-4 (2.105± 0.315). A very strong positive correlation was found between IL-1α in epithelium and IL-1α in connective tissue (R=0.855), as well as between IL-6 and IL-7 (R=0.865), both in connective tissues. The increase of IL-1α, IL-4, IL-6, IL-7, IL-8 and IL-17 positive structures suggest the role of these interleukins in the morphopathogenesis of calculous cholecystitis. The variable appearance of IL-1α, IL-4, IL-6, IL-7, IL-8 and IL-17 positive cells in connective tissue proves multi functional interleukin role there, while the epithelial distribution of IL-1α and IL-17 proves their selective role in the inflammation and immune system activation in the border tissue.
Original languageEnglish
Publication statusPublished - 24 Mar 2021
EventRSU Research week 2021: Knowledge for Use in Practice - Rīga, Latvia
Duration: 24 Mar 202126 Mar 2021


ConferenceRSU Research week 2021: Knowledge for Use in Practice
Abbreviated titleRW2021
Internet address

Field of Science*

  • 3.1 Basic medicine

Publication Type*

  • 3.4. Other publications in conference proceedings (including local)


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