Abstract
CD8+ T cells are the main effector cells of anti-cancer immune response that can be regulated by various costimulatory and coinhibitory molecules, including members of the B7 family. B7 homolog 3 (B7-H3) appears as a promising marker for immunotherapy; however, its significance in gastric cancer (GC) is unclear yet. We evaluated the spatial distribution of CD8+ T cells in relation to the expression of B7-H3 by double immunohistochemical staining. The level of B7-H3 intensity was scored manually (0-3) and dichotomized into B7-H3-low and B7-H3-high groups. The distribution and density of CD8+ T cells was analysed using whole slide digital imaging. B7-H3 was expressed mainly in the stromal compartment of GC (n = 73, 76% of all cases). Tumours with high expression of B7-H3 showed larger spatial differences of CD8+ T cells (86.4/mm2 in tumour centre vs. 414.9/mm2 in invasive front) when compared to B7-H3-low group (157.7/mm2 vs. 218.7/mm2, respectively) (p < 0.001). This study provides insight into the expression pattern of B7-H3 in GC of Western origin. In GCs with higher level of B7-H3 expression, CD8+ T cells were spatially suppressed in the tumour centre suggesting that B7-H3 might be involved in tumour escape mechanisms from the immune response.
Original language | English |
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Article number | 2129 |
Pages (from-to) | 1-8 |
Number of pages | 8 |
Journal | International Journal of Molecular Sciences |
Volume | 22 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2 Feb 2021 |
Keywords*
- B7-H3
- CD8-Positive T-Lymphocytes
- Gastric cancer
- Immune checkpoint
- Immune evasion
- Immunohistochemistry
- Tumour escape
- Tumour microenvironment
Field of Science*
- 3.1 Basic medicine
Publication Type*
- 1.1. Scientific article indexed in Web of Science and/or Scopus database