Genetic diversity and phylogenetic analysis of human bocavirus 2 in pediatric patients with acute gastroenteritis in Taiwan

Marco Yung Cheng Lin, Hsiu Chuan Chan, Hsin Chi, Shu Chun Chiu, Zaiga Nora-Krukle, Santa Rasa-Dzelzkaleja, Anda Vilmane, Modra Murovska, Jih Hui Lin (Coresponding Author), Hsin Fu Liu (Coresponding Author)

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Human bocavirus (HBoV) is a causative agent of respiratory and gastrointestinal diseases worldwide. Four HBoV species (HBoV1-4) have been identified so far. Although a previous report has documented the HBoV association with acute gastroenteritis (AGE) in Taiwan, their epidemiology, genetic diversity, and phylogenetic relationships remain unclear. In this study, we focused on an investigation of these unsolved issues, which will help to reveal molecular epidemiology and phylogeny of the circulating HBoV2 in Taiwan. A total of 176 stool samples were collected from children with AGE for this study. PCR amplification and sequencing on the VP1 gene region were used to identify species. Phylogenetic analysis was conducted by maximum-likelihood and neighbor-joining methods. Selection pressure was also estimated to obtain HBoV evolutionary information. Our results showed the prevalence of HBoV in AGE children was 8.5%, of which HBoV1 was the predominant species (6.3%), followed by HBoV2 (2.3%). Phylogenetic analysis showed those Taiwanese HBoV2 strains have significant genetic variability and can be divided into two clusters. One belongs to HBoV2 genotype A and the other forms an independent unclassified cluster. The nucleotide distance between that independent cluster and the known HBoV2 genotypes was more than 5%, suggesting a new HBoV2 genotype. No positive selection site was found and the virus was under purifying selection. This is the first report to reveal HBoV2 genetic diversity and phylogenetic relationships among AGE children in Taiwan. We find that HBoV2 may have been introduced into the country by multiple origins, and a potential new HBoV2 genotype is proposed.

Original languageEnglish
Article number1086
JournalInternational Journal of Environmental Research and Public Health
Issue number3
Publication statusPublished - 1 Feb 2020


  • Acute gastroenteritis
  • Genotype
  • Human bocavirus 2
  • Phylogenetic analysis

Field of Science*

  • 1.6 Biological sciences
  • 3.3 Health sciences

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


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