TY - JOUR
T1 - Genotypic and phenotypic characteristics of aminoglycoside-resistant Mycobacterium tuberculosis isolates in Latvia
AU - Bauskenieks, Matiss
AU - Pole, Ilva
AU - Skenders, Girts
AU - Jansone, Inta
AU - Broka, Lonija
AU - Nodieva, Anda
AU - Ozere, Iveta
AU - Kalvisa, Adrija
AU - Ranka, Renate
AU - Baumanis, Viesturs
N1 - Funding Information:
The authors are grateful for the support of grant of the Latvian Council of Science 09.1296 and VPP “BIOMEDICINE”. The authors would like to acknowledge the help of Dr Ivita Šēla given to the study.
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Mutations causing resistance to aminoglycosides, such as kanamycin (KAN), amikacin (AMK), and streptomycin, are not completely understood. In this study, polymorphisms of aminoglycoside resistance influencing genes such as rrs, eis, rpsL, and gidB in 41 drug-resistant and 17 pan-sensitive Mycobacterium tuberculosis clinical isolates in Latvia were analyzed. Mutation A1400G in rrs gene was detected in 92% isolates with high resistance level to KAN and diverse MIC level to AMK. Mutations in promoter region of eis were detected in 80% isolates with low-level MIC of KAN. The association of K43R mutation in rpsL gene, a mutation in the rrs gene at position 513, and various polymorphisms in gidB gene with distinct genetic lineages of M. tuberculosis was observed. The results of this study suggest that association of different controversial mutations of M. tuberculosis genes to the drug resistance phenotype should be done in respect to genetic lineages.
AB - Mutations causing resistance to aminoglycosides, such as kanamycin (KAN), amikacin (AMK), and streptomycin, are not completely understood. In this study, polymorphisms of aminoglycoside resistance influencing genes such as rrs, eis, rpsL, and gidB in 41 drug-resistant and 17 pan-sensitive Mycobacterium tuberculosis clinical isolates in Latvia were analyzed. Mutation A1400G in rrs gene was detected in 92% isolates with high resistance level to KAN and diverse MIC level to AMK. Mutations in promoter region of eis were detected in 80% isolates with low-level MIC of KAN. The association of K43R mutation in rpsL gene, a mutation in the rrs gene at position 513, and various polymorphisms in gidB gene with distinct genetic lineages of M. tuberculosis was observed. The results of this study suggest that association of different controversial mutations of M. tuberculosis genes to the drug resistance phenotype should be done in respect to genetic lineages.
KW - Aminoglycosides
KW - Latvia
KW - Mycobacterium tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=84925085929&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2014.12.004
DO - 10.1016/j.diagmicrobio.2014.12.004
M3 - Article
C2 - 25557624
AN - SCOPUS:84925085929
SN - 0732-8893
VL - 81
SP - 177
EP - 182
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 3
ER -
