Gestational Covid19: morphological alterations and decreased HLA-G expression caused by SARS-COV-2 infection

Silvia   Beltrami; Giovanna  Schiuma; Sabrina  Rizzo; Roberta  Rizzo; Angelina  Passaro; Pantaleo  Greco; Roberta  Gafà; Sandra Skuja; Daria  Bortolotti; Valērija Groma

Abstract

The evaluation of the effect of SARS-CoV-2 infection during pregnancy has raised interest. Even if virus vertical transmission is still controversial, several researchers have focused on the possible distinctive markers associated with different susceptibility to SARS-CoV-2 infection during pregnancy. Morphological alterations were assessed in the placental / chorionic villi,chorionic plate, basal plate, and umbilical cord tissues obtained from 7 subjects with symptomatic respiratory SARS-CoV-2 infection and compared with those in 7 non-COVID control subjects. The expression of SARS-CoV-2 Nucleoprotein (NP) and Human Leukocyte Antigen-G (HLA-G) was estimated by the use of immunohistochemistry. The 57%, 42,8%, and 28,6% of placental / chorionic villi, chorionic plate, and basal plate, respectively, were found positive for NP antigen (p<0.01), while none of the umbilical cords stained for NP. Placental / chorionic villi samples showed the highest positivity for NP. The presence of NP positivity correlated with high levels of the fibrinoid component in placental / chorionic villi samples and leukocyte infiltration in basal plate. All placental / chorionic villi samples were found positive for HLA-G, independently from NP staining. All the NP positive chorionic plate and half of the NP positive basal plate samples expressed HLA-G. On the contrary, the placental / chorionic villi, chorionic plate, and basal plate of all non-COVID subjects were positive for HLA-G, with a higher H-score in comparison to pathological samples (p<0.05). The presence of SARS-CoV-2 NP expression in gestational tissues correlates with morphological alterations and a decreased HLA-G expression compared to the ontrol group. These data suggest a possible implication of SARS-CoV-2 infection in morphological and protein expression modification during pregnancy, which might impact on infection susceptibility, pregnancy complications, and vertical transmission.

Original language	English
Pages	24-24
Number of pages	1
Publication status	Published - 2023
Event	2nd Conference of the World Sociey for Virology (WSV): One Health - One World-One Virology - Riga Stradiņš University, Dzirciema St. 16, Riga, Latvia Duration: 15 Jun 2023 → 17 Jun 2023 Conference number: 2 https://www.wsv2023.com/event-details/wsv2023-conference-1 https://www.wsv2023.com/ https://www.wsv2023.com/full-program

Conference

Conference	2nd Conference of the World Sociey for Virology (WSV)
Abbreviated title	WSV2023
Country/Territory	Latvia
City	Riga
Period	15/06/23 → 17/06/23
Internet address	https://www.wsv2023.com/event-details/wsv2023-conference-1 https://www.wsv2023.com/ https://www.wsv2023.com/full-program

Keywords*

Covid-19
pregnancy
HLA-G
immunohistochemistry

Field of Science*

3.1 Basic medicine

Publication Type*

3.4. Other publications in conference proceedings (including local)

Access to Document

Gestational Covid19Final published version, 261 KB
18 June Abstract-Booklet_MSV

Cite this

@conference{41a829edd9594a6eb9b22f14423f1e7b,

title = "Gestational Covid19: morphological alterations and decreased HLA-G expression caused by SARS-COV-2 infection",

abstract = "The evaluation of the effect of SARS-CoV-2 infection during pregnancy has raised interest. Even if virus vertical transmission is still controversial, several researchers have focused on the possible distinctive markers associated with different susceptibility to SARS-CoV-2 infection during pregnancy. Morphological alterations were assessed in the placental / chorionic villi,chorionic plate, basal plate, and umbilical cord tissues obtained from 7 subjects with symptomatic respiratory SARS-CoV-2 infection and compared with those in 7 non-COVID control subjects. The expression of SARS-CoV-2 Nucleoprotein (NP) and Human Leukocyte Antigen-G (HLA-G) was estimated by the use of immunohistochemistry. The 57%, 42,8%, and 28,6% of placental / chorionic villi, chorionic plate, and basal plate, respectively, were found positive for NP antigen (p<0.01), while none of the umbilical cords stained for NP. Placental / chorionic villi samples showed the highest positivity for NP. The presence of NP positivity correlated with high levels of the fibrinoid component in placental / chorionic villi samples and leukocyte infiltration in basal plate. All placental / chorionic villi samples were found positive for HLA-G, independently from NP staining. All the NP positive chorionic plate and half of the NP positive basal plate samples expressed HLA-G. On the contrary, the placental / chorionic villi, chorionic plate, and basal plate of all non-COVID subjects were positive for HLA-G, with a higher H-score in comparison to pathological samples (p<0.05). The presence of SARS-CoV-2 NP expression in gestational tissues correlates with morphological alterations and a decreased HLA-G expression compared to the ontrol group. These data suggest a possible implication of SARS-CoV-2 infection in morphological and protein expression modification during pregnancy, which might impact on infection susceptibility, pregnancy complications, and vertical transmission. ",

keywords = "Covid-19, pregnancy, HLA-G, immunohistochemistry",

author = "Silvia Beltrami and Giovanna Schiuma and Sabrina Rizzo and Roberta Rizzo and Angelina Passaro and Pantaleo Greco and Roberta Gaf{\`a} and Sandra Skuja and Daria Bortolotti and Valērija Groma",

year = "2023",

language = "English",

pages = "24--24",

note = "2nd Conference of the World Sociey for Virology (WSV) : One Health - One World-One Virology, WSV2023 ; Conference date: 15-06-2023 Through 17-06-2023",

url = "https://www.wsv2023.com/event-details/wsv2023-conference-1, https://www.wsv2023.com/, https://www.wsv2023.com/full-program",

}

TY - CONF

T1 - Gestational Covid19

T2 - 2nd Conference of the World Sociey for Virology (WSV)

AU - Beltrami, Silvia

AU - Schiuma, Giovanna

AU - Rizzo, Sabrina

AU - Rizzo, Roberta

AU - Passaro, Angelina

AU - Greco, Pantaleo

AU - Gafà, Roberta

AU - Skuja, Sandra

AU - Bortolotti, Daria

AU - Groma, Valērija

N1 - Conference code: 2

PY - 2023

Y1 - 2023

N2 - The evaluation of the effect of SARS-CoV-2 infection during pregnancy has raised interest. Even if virus vertical transmission is still controversial, several researchers have focused on the possible distinctive markers associated with different susceptibility to SARS-CoV-2 infection during pregnancy. Morphological alterations were assessed in the placental / chorionic villi,chorionic plate, basal plate, and umbilical cord tissues obtained from 7 subjects with symptomatic respiratory SARS-CoV-2 infection and compared with those in 7 non-COVID control subjects. The expression of SARS-CoV-2 Nucleoprotein (NP) and Human Leukocyte Antigen-G (HLA-G) was estimated by the use of immunohistochemistry. The 57%, 42,8%, and 28,6% of placental / chorionic villi, chorionic plate, and basal plate, respectively, were found positive for NP antigen (p<0.01), while none of the umbilical cords stained for NP. Placental / chorionic villi samples showed the highest positivity for NP. The presence of NP positivity correlated with high levels of the fibrinoid component in placental / chorionic villi samples and leukocyte infiltration in basal plate. All placental / chorionic villi samples were found positive for HLA-G, independently from NP staining. All the NP positive chorionic plate and half of the NP positive basal plate samples expressed HLA-G. On the contrary, the placental / chorionic villi, chorionic plate, and basal plate of all non-COVID subjects were positive for HLA-G, with a higher H-score in comparison to pathological samples (p<0.05). The presence of SARS-CoV-2 NP expression in gestational tissues correlates with morphological alterations and a decreased HLA-G expression compared to the ontrol group. These data suggest a possible implication of SARS-CoV-2 infection in morphological and protein expression modification during pregnancy, which might impact on infection susceptibility, pregnancy complications, and vertical transmission.

AB - The evaluation of the effect of SARS-CoV-2 infection during pregnancy has raised interest. Even if virus vertical transmission is still controversial, several researchers have focused on the possible distinctive markers associated with different susceptibility to SARS-CoV-2 infection during pregnancy. Morphological alterations were assessed in the placental / chorionic villi,chorionic plate, basal plate, and umbilical cord tissues obtained from 7 subjects with symptomatic respiratory SARS-CoV-2 infection and compared with those in 7 non-COVID control subjects. The expression of SARS-CoV-2 Nucleoprotein (NP) and Human Leukocyte Antigen-G (HLA-G) was estimated by the use of immunohistochemistry. The 57%, 42,8%, and 28,6% of placental / chorionic villi, chorionic plate, and basal plate, respectively, were found positive for NP antigen (p<0.01), while none of the umbilical cords stained for NP. Placental / chorionic villi samples showed the highest positivity for NP. The presence of NP positivity correlated with high levels of the fibrinoid component in placental / chorionic villi samples and leukocyte infiltration in basal plate. All placental / chorionic villi samples were found positive for HLA-G, independently from NP staining. All the NP positive chorionic plate and half of the NP positive basal plate samples expressed HLA-G. On the contrary, the placental / chorionic villi, chorionic plate, and basal plate of all non-COVID subjects were positive for HLA-G, with a higher H-score in comparison to pathological samples (p<0.05). The presence of SARS-CoV-2 NP expression in gestational tissues correlates with morphological alterations and a decreased HLA-G expression compared to the ontrol group. These data suggest a possible implication of SARS-CoV-2 infection in morphological and protein expression modification during pregnancy, which might impact on infection susceptibility, pregnancy complications, and vertical transmission.

KW - Covid-19

KW - pregnancy

KW - HLA-G

KW - immunohistochemistry

UR - https://www.wsv2023.com/

M3 - Abstract

SP - 24

EP - 24

Y2 - 15 June 2023 through 17 June 2023

ER -