Gestational Covid19: morphological alterations and decreased HLA-G expression caused by SARS-COV-2 infection

Silvia Beltrami, Giovanna Schiuma, Sabrina Rizzo, Roberta Rizzo, Angelina Passaro, Pantaleo Greco, Roberta Gafà, Sandra Skuja, Daria Bortolotti, Valērija Groma

Research output: Contribution to conferenceAbstractpeer-review

16 Downloads (Pure)


The evaluation of the effect of SARS-CoV-2 infection during pregnancy has raised interest. Even if virus vertical transmission is still controversial, several researchers have focused on the possible distinctive markers associated with different susceptibility to SARS-CoV-2 infection during pregnancy. Morphological alterations were assessed in the placental / chorionic villi,chorionic plate, basal plate, and umbilical cord tissues obtained from 7 subjects with symptomatic respiratory SARS-CoV-2 infection and compared with those in 7 non-COVID control subjects. The expression of SARS-CoV-2 Nucleoprotein (NP) and Human Leukocyte Antigen-G (HLA-G) was estimated by the use of immunohistochemistry. The 57%, 42,8%, and 28,6% of placental / chorionic villi, chorionic plate, and basal plate, respectively, were found positive for NP antigen (p<0.01), while none of the umbilical cords stained for NP. Placental / chorionic villi samples showed the highest positivity for NP. The presence of NP positivity correlated with high levels of the fibrinoid component in placental / chorionic villi samples and leukocyte infiltration in basal plate. All placental / chorionic villi samples were found positive for HLA-G, independently from NP staining. All the NP positive chorionic plate and half of the NP positive basal plate samples expressed HLA-G. On the contrary, the placental / chorionic villi, chorionic plate, and basal plate of all non-COVID subjects were positive for HLA-G, with a higher H-score in comparison to pathological samples (p<0.05). The presence of SARS-CoV-2 NP expression in gestational tissues correlates with morphological alterations and a decreased HLA-G expression compared to the ontrol group. These data suggest a possible implication of SARS-CoV-2 infection in morphological and protein expression modification during pregnancy, which might impact on infection susceptibility, pregnancy complications, and vertical transmission.

Original languageEnglish
Number of pages1
Publication statusPublished - 2023
Event2nd Conference of the World Sociey for Virology (WSV): One Health - One World-One Virology - Riga Stradiņš University, Dzirciema St. 16, Riga, Latvia
Duration: 15 Jun 202317 Jun 2023
Conference number: 2


Conference2nd Conference of the World Sociey for Virology (WSV)
Abbreviated titleWSV2023
Internet address


  • Covid-19
  • pregnancy
  • HLA-G
  • immunohistochemistry

Field of Science*

  • 3.1 Basic medicine

Publication Type*

  • 3.4. Other publications in conference proceedings (including local)


Dive into the research topics of 'Gestational Covid19: morphological alterations and decreased HLA-G expression caused by SARS-COV-2 infection'. Together they form a unique fingerprint.

Cite this