Glyoxalase 1 and glyoxalase 2 activities in blood and neuronal tissue samples from experimental animal models of obesity and type 2 diabetes mellitus

Elina Skapare, Ilze Konrade, Edgars Liepinsh, Marina Makrecka, Liga Zvejniece, Baiba Svalbe, Reinis Vilskersts, Maija Dambrova

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The glyoxalase enzymes catalyse the conversion of reactive glucose metabolites into non-toxic products as a part of the cellular defence system against glycation. This study investigated changes in glyoxalase 1 and glyoxalase 2 activities and the development of diabetic complications in experimental animal models of obesity (Zucker fa/fa rats) and type 2 diabetes mellitus (Goto- Kakizaki rats). In contrast to Zucker rats, in Goto-Kakizaki rats the glyoxalase 1 activities in brain, spinal cord and sciatic nerve tissues were significantly reduced by 10, 32 and 36 %, respectively. Lower glyoxalase 1 activity in the neuronal tissues was associated with a higher blood glucose concentration and impaired endothelium-dependent relaxation to acetylcholine in aortic rings in Goto-Kakizaki rats. This study provides evidence for disturbed neuronal glyoxalase 1 activity under conditions of hyperglycaemia in the presence of impaired endothelium-dependent relaxation and cognitive function.

Original languageEnglish
Pages (from-to)469-478
Number of pages10
JournalJournal of Physiological Sciences
Volume62
Issue number6
DOIs
Publication statusPublished - Nov 2012

Keywords

  • Glyoxalase
  • Goto-Kakizaki rats
  • Late diabetic complications
  • Obesity
  • Working memory

Field of Science

  • 3.1 Basic medicine

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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