Growth of tobacco protoplasts stimulated by synthetic lipo- chitooligosaccharides

Horst Röhrig, Jürgen Schmidt, Richard Walden, Inge Czaja, Edvins Miklaševičs, Ursula Wieneke, Jeff Schell, Michael John

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Nodulation (Nod) factors are lipo-chitooligosaccharides (LCOs) secreted by rhizobia to trigger the early steps of nodule organogenesis in leguminous plants. A method to synthesize LCOs in vitro was developed. Synthetic LCOs alleviated the requirement for auxin and cytokinin to sustain growth of cultured tobacco protoplasts. LCOs containing C18:1 trans-fatty acyl substituents were more effective than those containing cis-fatty acids in promoting cell division as well as in activating an auxin-responsive promoter and the expression of a gene implicated in auxin action. These data indicate that LCOs redirect plant growth also in nonlegumes by activating developmental pathways also targeted by phytohormones.

Original languageEnglish
Pages (from-to)841-843
Number of pages3
Issue number5225
Publication statusPublished - 1995
Externally publishedYes


  • medicine

Field of Science*

  • 3.1 Basic medicine
  • 1.6 Biological sciences

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


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