Growth of tobacco protoplasts stimulated by synthetic lipo- chitooligosaccharides

Horst Röhrig; Jürgen Schmidt; Richard Walden; Inge Czaja; Edvins Miklaševičs; Ursula Wieneke; Jeff Schell; Michael John

doi:10.1126/science.269.5225.841

Growth of tobacco protoplasts stimulated by synthetic lipo- chitooligosaccharides

Horst Röhrig
, Jürgen Schmidt
, Richard Walden
, Inge Czaja
, Edvins Miklaševičs
, Ursula Wieneke
, Jeff Schell
, Michael John

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

Nodulation (Nod) factors are lipo-chitooligosaccharides (LCOs) secreted by rhizobia to trigger the early steps of nodule organogenesis in leguminous plants. A method to synthesize LCOs in vitro was developed. Synthetic LCOs alleviated the requirement for auxin and cytokinin to sustain growth of cultured tobacco protoplasts. LCOs containing C_18:1 trans-fatty acyl substituents were more effective than those containing cis-fatty acids in promoting cell division as well as in activating an auxin-responsive promoter and the expression of a gene implicated in auxin action. These data indicate that LCOs redirect plant growth also in nonlegumes by activating developmental pathways also targeted by phytohormones.

Original language	English
Pages (from-to)	841-843
Number of pages	3
Journal	Science
Volume	269
Issue number	5225
DOIs	https://doi.org/10.1126/science.269.5225.841
Publication status	Published - 1995
Externally published	Yes

Keywords*

medicine

Field of Science*

3.1 Basic medicine
1.6 Biological sciences

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1126/science.269.5225.841

Cite this

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title = "Growth of tobacco protoplasts stimulated by synthetic lipo- chitooligosaccharides",

abstract = "Nodulation (Nod) factors are lipo-chitooligosaccharides (LCOs) secreted by rhizobia to trigger the early steps of nodule organogenesis in leguminous plants. A method to synthesize LCOs in vitro was developed. Synthetic LCOs alleviated the requirement for auxin and cytokinin to sustain growth of cultured tobacco protoplasts. LCOs containing C18:1 trans-fatty acyl substituents were more effective than those containing cis-fatty acids in promoting cell division as well as in activating an auxin-responsive promoter and the expression of a gene implicated in auxin action. These data indicate that LCOs redirect plant growth also in nonlegumes by activating developmental pathways also targeted by phytohormones.",

keywords = "medicine",

author = "Horst R{\"o}hrig and J{\"u}rgen Schmidt and Richard Walden and Inge Czaja and Edvins Mikla{\v s}evi{\v c}s and Ursula Wieneke and Jeff Schell and Michael John",

year = "1995",

doi = "10.1126/science.269.5225.841",

language = "English",

volume = "269",

pages = "841--843",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5225",

}

TY - JOUR

T1 - Growth of tobacco protoplasts stimulated by synthetic lipo- chitooligosaccharides

AU - Röhrig, Horst

AU - Schmidt, Jürgen

AU - Walden, Richard

AU - Czaja, Inge

AU - Miklaševičs, Edvins

AU - Wieneke, Ursula

AU - Schell, Jeff

AU - John, Michael

PY - 1995

Y1 - 1995

N2 - Nodulation (Nod) factors are lipo-chitooligosaccharides (LCOs) secreted by rhizobia to trigger the early steps of nodule organogenesis in leguminous plants. A method to synthesize LCOs in vitro was developed. Synthetic LCOs alleviated the requirement for auxin and cytokinin to sustain growth of cultured tobacco protoplasts. LCOs containing C18:1 trans-fatty acyl substituents were more effective than those containing cis-fatty acids in promoting cell division as well as in activating an auxin-responsive promoter and the expression of a gene implicated in auxin action. These data indicate that LCOs redirect plant growth also in nonlegumes by activating developmental pathways also targeted by phytohormones.

AB - Nodulation (Nod) factors are lipo-chitooligosaccharides (LCOs) secreted by rhizobia to trigger the early steps of nodule organogenesis in leguminous plants. A method to synthesize LCOs in vitro was developed. Synthetic LCOs alleviated the requirement for auxin and cytokinin to sustain growth of cultured tobacco protoplasts. LCOs containing C18:1 trans-fatty acyl substituents were more effective than those containing cis-fatty acids in promoting cell division as well as in activating an auxin-responsive promoter and the expression of a gene implicated in auxin action. These data indicate that LCOs redirect plant growth also in nonlegumes by activating developmental pathways also targeted by phytohormones.

KW - medicine

UR - https://www.scopus.com/pages/publications/0029147606

U2 - 10.1126/science.269.5225.841

DO - 10.1126/science.269.5225.841

M3 - Article

AN - SCOPUS:0029147606

SN - 0036-8075

VL - 269

SP - 841

EP - 843

JO - Science

JF - Science

IS - 5225

ER -

Growth of tobacco protoplasts stimulated by synthetic lipo- chitooligosaccharides

Abstract

Keywords*

Field of Science*

Publication Type*

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