Human/mouse microcell hybrid based elimination test reduces the putative tumor suppressor region at 3p21.3 to 1.6 cM

Anna Szeles, Ying Yang, Agneta Manneborg Sandlund, Irina Kholodnyuk, Hajnalka Kiss, Maria Kost-Alimova, Eugene R. Zabarovsky, Eric Stanbridge, George Klein, Stephan Imreh

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

We have previously identified an approximately 7 cM long common eliminated region (CER), involving the 3p21.3 markers AP20R, D3S966, D3S3559, D3S1029, WI-7947, D3S2354, AFNb362wb9, and D3S32, in human chromosome 3/A9 mouse fibrosarcoma microcell hybrid (MCH) derived SCID mouse tumors. We now report the results of our more detailed analysis on 24 SCID mouse tumors derived from two PICH lines that originally carried intact human chromosomes 3. They were analyzed by fluorescence in situ hybridization (FISH) painting and PCR, using 24 markers covering the region between D3S1611 and D3S 1235 at 3p22-p21.2. D3S32 and D3S2354 were regularly eliminated during in vivo tumor growth, whereas the other 22 markers, D3S1611, ACAA, D3S1260, WI-692, AP20R, D3S3521, D3S966, D3S1029, D3S643, WI-2420, MST1, GNA12, D3S1235, D3S 1298, GLB1, WI-4193, D3S3658, D3S3559, D3S3678, WI-6400, WI-7947, and WI-10665, were regularly retained. We have defined a common eliminated region of approximately 1.6 cM (designated as CERI) inside the 7 cM CER described earlier. CERI is flanked distally by D3S1029 and proximally by D3S643.

Original languageEnglish
Pages (from-to)329-336
Number of pages8
JournalGenes Chromosomes and Cancer
Volume20
Issue number4
DOIs
Publication statusPublished - Dec 1997

Field of Science

  • 1.6 Biological sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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