Human/mouse microcell hybrid based elimination test reduces the putative tumor suppressor region at 3p21.3 to 1.6 cM

Anna Szeles; Ying Yang; Agneta Manneborg Sandlund; Irina Kholodnyuk; Hajnalka Kiss; Maria Kost-Alimova; Eugene R. Zabarovsky; Eric Stanbridge; George Klein; Stephan Imreh

doi:10.1002/(SICI)1098-2264(199712)20:4<329::AID-GCC3>3.0.CO;2-3

Human/mouse microcell hybrid based elimination test reduces the putative tumor suppressor region at 3p21.3 to 1.6 cM

Anna Szeles^*
, Ying Yang
, Agneta Manneborg Sandlund
, Irina Kholodnyuk
, Hajnalka Kiss
, Maria Kost-Alimova
, Eugene R. Zabarovsky
, Eric Stanbridge
, George Klein
, Stephan Imreh

^*Corresponding author for this work

Institute of Microbiology and Virology

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

We have previously identified an approximately 7 cM long common eliminated region (CER), involving the 3p21.3 markers AP20R, D3S966, D3S3559, D3S1029, WI-7947, D3S2354, AFNb362wb9, and D3S32, in human chromosome 3/A9 mouse fibrosarcoma microcell hybrid (MCH) derived SCID mouse tumors. We now report the results of our more detailed analysis on 24 SCID mouse tumors derived from two PICH lines that originally carried intact human chromosomes 3. They were analyzed by fluorescence in situ hybridization (FISH) painting and PCR, using 24 markers covering the region between D3S1611 and D3S 1235 at 3p22-p21.2. D3S32 and D3S2354 were regularly eliminated during in vivo tumor growth, whereas the other 22 markers, D3S1611, ACAA, D3S1260, WI-692, AP20R, D3S3521, D3S966, D3S1029, D3S643, WI-2420, MST1, GNA12, D3S1235, D3S 1298, GLB1, WI-4193, D3S3658, D3S3559, D3S3678, WI-6400, WI-7947, and WI-10665, were regularly retained. We have defined a common eliminated region of approximately 1.6 cM (designated as CERI) inside the 7 cM CER described earlier. CERI is flanked distally by D3S1029 and proximally by D3S643.

Original language	English
Pages (from-to)	329-336
Number of pages	8
Journal	Genes Chromosomes and Cancer
Volume	20
Issue number	4
DOIs	https://doi.org/10.1002/(SICI)1098-2264(199712)20:4<329::AID-GCC3>3.0.CO;2-3
Publication status	Published - Dec 1997

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Field of Science*

1.6 Biological sciences

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

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10.1002/(SICI)1098-2264(199712)20:4<329::AID-GCC3>3.0.CO;2-3

https://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2264(199712)20:4%3C329::AID-GCC3%3E3.0.CO;2-3

Cite this

Szeles, A., Yang, Y., Sandlund, A. M., Kholodnyuk, I., Kiss, H., Kost-Alimova, M., Zabarovsky, E. R., Stanbridge, E., Klein, G., & Imreh, S. (1997). Human/mouse microcell hybrid based elimination test reduces the putative tumor suppressor region at 3p21.3 to 1.6 cM. Genes Chromosomes and Cancer, 20(4), 329-336. https://doi.org/10.1002/(SICI)1098-2264(199712)20:4<329::AID-GCC3>3.0.CO;2-3

@article{5ef31406a6334be5919f8b812b4f90e0,

title = "Human/mouse microcell hybrid based elimination test reduces the putative tumor suppressor region at 3p21.3 to 1.6 cM",

abstract = "We have previously identified an approximately 7 cM long common eliminated region (CER), involving the 3p21.3 markers AP20R, D3S966, D3S3559, D3S1029, WI-7947, D3S2354, AFNb362wb9, and D3S32, in human chromosome 3/A9 mouse fibrosarcoma microcell hybrid (MCH) derived SCID mouse tumors. We now report the results of our more detailed analysis on 24 SCID mouse tumors derived from two PICH lines that originally carried intact human chromosomes 3. They were analyzed by fluorescence in situ hybridization (FISH) painting and PCR, using 24 markers covering the region between D3S1611 and D3S 1235 at 3p22-p21.2. D3S32 and D3S2354 were regularly eliminated during in vivo tumor growth, whereas the other 22 markers, D3S1611, ACAA, D3S1260, WI-692, AP20R, D3S3521, D3S966, D3S1029, D3S643, WI-2420, MST1, GNA12, D3S1235, D3S 1298, GLB1, WI-4193, D3S3658, D3S3559, D3S3678, WI-6400, WI-7947, and WI-10665, were regularly retained. We have defined a common eliminated region of approximately 1.6 cM (designated as CERI) inside the 7 cM CER described earlier. CERI is flanked distally by D3S1029 and proximally by D3S643.",

author = "Anna Szeles and Ying Yang and Sandlund, \{Agneta Manneborg\} and Irina Kholodnyuk and Hajnalka Kiss and Maria Kost-Alimova and Zabarovsky, \{Eugene R.\} and Eric Stanbridge and George Klein and Stephan Imreh",

year = "1997",

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doi = "10.1002/(SICI)1098-2264(199712)20:4<329::AID-GCC3>3.0.CO;2-3",

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Szeles, A, Yang, Y, Sandlund, AM, Kholodnyuk, I, Kiss, H, Kost-Alimova, M, Zabarovsky, ER, Stanbridge, E, Klein, G & Imreh, S 1997, 'Human/mouse microcell hybrid based elimination test reduces the putative tumor suppressor region at 3p21.3 to 1.6 cM', Genes Chromosomes and Cancer, vol. 20, no. 4, pp. 329-336. https://doi.org/10.1002/(SICI)1098-2264(199712)20:4<329::AID-GCC3>3.0.CO;2-3

TY - JOUR

T1 - Human/mouse microcell hybrid based elimination test reduces the putative tumor suppressor region at 3p21.3 to 1.6 cM

AU - Szeles, Anna

AU - Yang, Ying

AU - Sandlund, Agneta Manneborg

AU - Kholodnyuk, Irina

AU - Kiss, Hajnalka

AU - Kost-Alimova, Maria

AU - Zabarovsky, Eugene R.

AU - Stanbridge, Eric

AU - Klein, George

AU - Imreh, Stephan

PY - 1997/12

Y1 - 1997/12

N2 - We have previously identified an approximately 7 cM long common eliminated region (CER), involving the 3p21.3 markers AP20R, D3S966, D3S3559, D3S1029, WI-7947, D3S2354, AFNb362wb9, and D3S32, in human chromosome 3/A9 mouse fibrosarcoma microcell hybrid (MCH) derived SCID mouse tumors. We now report the results of our more detailed analysis on 24 SCID mouse tumors derived from two PICH lines that originally carried intact human chromosomes 3. They were analyzed by fluorescence in situ hybridization (FISH) painting and PCR, using 24 markers covering the region between D3S1611 and D3S 1235 at 3p22-p21.2. D3S32 and D3S2354 were regularly eliminated during in vivo tumor growth, whereas the other 22 markers, D3S1611, ACAA, D3S1260, WI-692, AP20R, D3S3521, D3S966, D3S1029, D3S643, WI-2420, MST1, GNA12, D3S1235, D3S 1298, GLB1, WI-4193, D3S3658, D3S3559, D3S3678, WI-6400, WI-7947, and WI-10665, were regularly retained. We have defined a common eliminated region of approximately 1.6 cM (designated as CERI) inside the 7 cM CER described earlier. CERI is flanked distally by D3S1029 and proximally by D3S643.

AB - We have previously identified an approximately 7 cM long common eliminated region (CER), involving the 3p21.3 markers AP20R, D3S966, D3S3559, D3S1029, WI-7947, D3S2354, AFNb362wb9, and D3S32, in human chromosome 3/A9 mouse fibrosarcoma microcell hybrid (MCH) derived SCID mouse tumors. We now report the results of our more detailed analysis on 24 SCID mouse tumors derived from two PICH lines that originally carried intact human chromosomes 3. They were analyzed by fluorescence in situ hybridization (FISH) painting and PCR, using 24 markers covering the region between D3S1611 and D3S 1235 at 3p22-p21.2. D3S32 and D3S2354 were regularly eliminated during in vivo tumor growth, whereas the other 22 markers, D3S1611, ACAA, D3S1260, WI-692, AP20R, D3S3521, D3S966, D3S1029, D3S643, WI-2420, MST1, GNA12, D3S1235, D3S 1298, GLB1, WI-4193, D3S3658, D3S3559, D3S3678, WI-6400, WI-7947, and WI-10665, were regularly retained. We have defined a common eliminated region of approximately 1.6 cM (designated as CERI) inside the 7 cM CER described earlier. CERI is flanked distally by D3S1029 and proximally by D3S643.

UR - https://www.scopus.com/pages/publications/0030726503

U2 - 10.1002/(SICI)1098-2264(199712)20:4<329::AID-GCC3>3.0.CO;2-3

DO - 10.1002/(SICI)1098-2264(199712)20:4<329::AID-GCC3>3.0.CO;2-3

M3 - Article

C2 - 9408748

AN - SCOPUS:0030726503

SN - 1045-2257

VL - 20

SP - 329

EP - 336

JO - Genes Chromosomes and Cancer

JF - Genes Chromosomes and Cancer

IS - 4

ER -

Human/mouse microcell hybrid based elimination test reduces the putative tumor suppressor region at 3p21.3 to 1.6 cM

Abstract

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