Identification and in silico characterization of novel and conservedmicrornas inmethyl jasmonate-stimulated scots pine (Pinus Sylvestris L.) needles

Baiba Krivmane, Ilze Šņepste, Vilnis Škipars, Igor Yakovlev, Carl Gunnar Fossdal, Adam Vivian-Smith, Dainis Ruņgis (Coresponding Author)

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
5 Downloads (Pure)


MicroRNAs (miRNAs) are non-protein coding RNAs of ~20-24 nucleotides in length that play an important role in many biological and metabolic processes, including the regulation of gene expression, plant growth and developmental processes, as well as responses to stress and pathogens. The aim of this study was to identify and characterize novel and conserved microRNAs expressed in methyl jasmonate-treated Scots pine needles. In addition, potential precursor sequences and target genes of the identified miRNAs were determined by alignment to the Pinus unigene set. Potential precursor sequences were identified using the miRAtool, conserved miRNA precursors were also tested for the ability to form the required stem-loop structure, and the minimal folding free energy indexes were calculated. By comparison with miRBase, 4975 annotated sequences were identified and assigned to 173 miRNA groups, belonging to a total of 60 conserved miRNA families. A total of 1029 potential novel miRNAs, grouped into 34 families were found, and 46 predicted precursor sequences were identified. A total of 136 potential target genes targeted by 28 families were identified. The majority of previously reported highly conserved plant miRNAs were identified in this study, as well as some conserved miRNAs previously reported to be monocot specific. No conserved dicot-specific miRNAs were identified. A number of potential gymnosperm or conifer specific miRNAs were found, shared among a range of conifer species.

Original languageEnglish
Article number384
Number of pages16
Issue number4
Publication statusPublished - 2020
Externally publishedYes


  • IontorrentPGM
  • IsomiR
  • Methyl jasmonate
  • MicroRNA
  • Precursor microRNA
  • Scots pine

Field of Science*

  • 4.1 Agriculture, Forestry, and Fisheries
  • 1.6 Biological sciences

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


Dive into the research topics of 'Identification and in silico characterization of novel and conservedmicrornas inmethyl jasmonate-stimulated scots pine (Pinus Sylvestris L.) needles'. Together they form a unique fingerprint.

Cite this