Identification of Inflammatory and Regulatory Cytokines IL-1α-, IL-4-, IL-6-, IL-12-, IL-13-, IL-17A-, TNF-α-, and IFN-γ-Producing Cells in the Milk of Dairy Cows with Subclinical and Clinical Mastitis

Zane Vitenberga-Verza (Coresponding Author), Māra Pilmane, Ksenija Šerstņova, Ivars Melderis, Łukasz Gontar, Maksymilian Kochanski, Andzelika Drutowska, Maroti Gergely, Beatrise Prieto-Simona

Research output: Contribution to journalArticlepeer-review

Abstract

In naturally occurring bovine mastitis, effects of infection depend on the host inflammatory response, including the effects of secreted cytokines. Knowledge about the inflammatory and regulatory cytokines in milk cells of free-stall barn dairy cows and in naturally occurring mastitis is lacking as most studies focus on induced mastitis. Hereby, the aim of the study was to determine
inflammatory and regulatory cytokines in the milk of dairy cows with subclinical and clinical mastitis.
The following examinations of milk samples were performed: differential counting of somatic cells (SCC), bacteriological examination, and immunocytochemical analysis. Mean SCC increased in subclinical and clinical mastitis cases. The number of pathogenic mastitis-causing bacteria on plates increased in subclinical mastitis cases but decreased in clinical mastitis. The inflammatory and regulatory markers in the milk cells of healthy cows showed the highest mean cell numbers (%). In mastitis cases, immunoreactivity was more pronounced for IL-4, IL-6, IL-12, IL-13, IL-17A, TNF-, and IFN-y. Data about subclinical and clinical mastitis demonstrate inflammatory responses to intramammary infection driven by IL-1, IL-4, and IL-17A. Moreover, the host defense response in mastitis is characterized by continuation or resolution of initial inflammation. IL-12 and INF-immunoreactivity was recognized to differ mastitis cases from the relative health status.
Original languageEnglish
Article number372
Number of pages23
JournalPathogens
Volume11
Issue number3
DOIs
Publication statusPublished - 17 Mar 2022

Keywords*

  • diagnosis
  • mastitis
  • bovine
  • cytokines
  • inflammation
  • immunohistochemistry
  • milk

Field of Science*

  • 3.1 Basic medicine

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

Fingerprint

Dive into the research topics of 'Identification of Inflammatory and Regulatory Cytokines IL-1α-, IL-4-, IL-6-, IL-12-, IL-13-, IL-17A-, TNF-α-, and IFN-γ-Producing Cells in the Milk of Dairy Cows with Subclinical and Clinical Mastitis'. Together they form a unique fingerprint.

Cite this