TY - JOUR
T1 - Identification of stress-tolerance-related transcription-factor genes via mini-scale Full-length cDNA Over-eXpressor (FOX) gene hunting system
AU - Fujita, Miki
AU - Mizukado, Saho
AU - Fujita, Yasunari
AU - Ichikawa, Takanari
AU - Nakazawa, Miki
AU - Seki, Motoaki
AU - Matsui, Minami
AU - Yamaguchi-Shinozaki, Kazuko
AU - Shinozaki, Kazuo
N1 - Funding Information:
We thank Ms. Hiroko Kobayashi, Ms. Machiko Ohnuki, Ms. Kumiko Matsuo, Ms. Michie Etoh, and Ms. Akiko Enju for their excellent technical assistance. We are grateful to Ms. Junko Ishida and Ms. Kyoko Yoshiwara for excellent support of microarray analysis. This work was supported in part by CREST and Genome Project of RIKEN. Appendix A
PY - 2007/12/14
Y1 - 2007/12/14
N2 - Recently, we developed a novel system known as Full-length cDNA Over-eXpressor (FOX) gene hunting [T. Ichikawa, M. Nakazawa, M. Kawashima, H. Iizumi, H. Kuroda, Y. Kondou, Y. Tsuhara, K. Suzuki, A. Ishikawa, M. Seki, M. Fujita, R. Motohashi, N. Nagata, T. Takagi, K. Shinozaki, M. Matsui, The FOX hunting system: an alternative gain-of-function gene hunting technique, Plant J. 48 (2006) 974-985], which involves the random overexpression of a normalized Arabidopsis full-length cDNA library. While our system allows large-scale collection of full-length cDNAs for gene discovery, we sought to downsize it to analyze a small pool of full-length cDNAs. As a model system, we focused on stress-inducible transcription factors. The full-length cDNAs of 43 stress-inducible transcription factors were mixed to create a transgenic plant library. We screened for salt-stress-resistant lines in the T1 generation and identified a number of salt-tolerant lines that harbored the same transgene (F39). F39 encodes a bZIP-type transcription factor that is identical to AtbZIP60, which is believed to be involved in the endoplasmic reticulum stress response. Microarray analysis revealed that a number of stress-inducible genes were up-regulated in the F39-overexpressing lines, suggesting that AtbZIP60 is involved in stress signal transduction. Thus, our mini-scale FOX system may be used to screen for genes with valuable functions, such as transcription factors, from a small pool of genes that show similar expression profiles.
AB - Recently, we developed a novel system known as Full-length cDNA Over-eXpressor (FOX) gene hunting [T. Ichikawa, M. Nakazawa, M. Kawashima, H. Iizumi, H. Kuroda, Y. Kondou, Y. Tsuhara, K. Suzuki, A. Ishikawa, M. Seki, M. Fujita, R. Motohashi, N. Nagata, T. Takagi, K. Shinozaki, M. Matsui, The FOX hunting system: an alternative gain-of-function gene hunting technique, Plant J. 48 (2006) 974-985], which involves the random overexpression of a normalized Arabidopsis full-length cDNA library. While our system allows large-scale collection of full-length cDNAs for gene discovery, we sought to downsize it to analyze a small pool of full-length cDNAs. As a model system, we focused on stress-inducible transcription factors. The full-length cDNAs of 43 stress-inducible transcription factors were mixed to create a transgenic plant library. We screened for salt-stress-resistant lines in the T1 generation and identified a number of salt-tolerant lines that harbored the same transgene (F39). F39 encodes a bZIP-type transcription factor that is identical to AtbZIP60, which is believed to be involved in the endoplasmic reticulum stress response. Microarray analysis revealed that a number of stress-inducible genes were up-regulated in the F39-overexpressing lines, suggesting that AtbZIP60 is involved in stress signal transduction. Thus, our mini-scale FOX system may be used to screen for genes with valuable functions, such as transcription factors, from a small pool of genes that show similar expression profiles.
KW - Arabidopsis
KW - Dominant phenotype
KW - Full-length cDNA
KW - Functional genomics
KW - Salt stress
KW - Stress tolerance
UR - http://www.scopus.com/inward/record.url?scp=35348960703&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.09.124
DO - 10.1016/j.bbrc.2007.09.124
M3 - Article
C2 - 17937930
AN - SCOPUS:35348960703
SN - 0006-291X
VL - 364
SP - 250
EP - 257
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -