Identification of stress-tolerance-related transcription-factor genes via mini-scale Full-length cDNA Over-eXpressor (FOX) gene hunting system

Miki Fujita, Saho Mizukado, Yasunari Fujita, Takanari Ichikawa, Miki Nakazawa, Motoaki Seki, Minami Matsui, Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki

Research output: Contribution to journalArticlepeer-review

83 Citations (Scopus)

Abstract

Recently, we developed a novel system known as Full-length cDNA Over-eXpressor (FOX) gene hunting [T. Ichikawa, M. Nakazawa, M. Kawashima, H. Iizumi, H. Kuroda, Y. Kondou, Y. Tsuhara, K. Suzuki, A. Ishikawa, M. Seki, M. Fujita, R. Motohashi, N. Nagata, T. Takagi, K. Shinozaki, M. Matsui, The FOX hunting system: an alternative gain-of-function gene hunting technique, Plant J. 48 (2006) 974-985], which involves the random overexpression of a normalized Arabidopsis full-length cDNA library. While our system allows large-scale collection of full-length cDNAs for gene discovery, we sought to downsize it to analyze a small pool of full-length cDNAs. As a model system, we focused on stress-inducible transcription factors. The full-length cDNAs of 43 stress-inducible transcription factors were mixed to create a transgenic plant library. We screened for salt-stress-resistant lines in the T1 generation and identified a number of salt-tolerant lines that harbored the same transgene (F39). F39 encodes a bZIP-type transcription factor that is identical to AtbZIP60, which is believed to be involved in the endoplasmic reticulum stress response. Microarray analysis revealed that a number of stress-inducible genes were up-regulated in the F39-overexpressing lines, suggesting that AtbZIP60 is involved in stress signal transduction. Thus, our mini-scale FOX system may be used to screen for genes with valuable functions, such as transcription factors, from a small pool of genes that show similar expression profiles.

Original languageEnglish
Pages (from-to)250-257
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume364
Issue number2
DOIs
Publication statusPublished - 14 Dec 2007
Externally publishedYes

Keywords

  • Arabidopsis
  • Dominant phenotype
  • Full-length cDNA
  • Functional genomics
  • Salt stress
  • Stress tolerance

Field of Science

  • 1.6 Biological sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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