Immobilisation increases yeast cells' resistance to dehydration-rehydration treatment

Diana Borovikova, Linda Rozenfelde, Ilona Pavlovska, Alexander Rapoport

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

This study was performed with the goal of revealing if the dehydration procedure used in our new immobilisation method noticeably decreases the viability of yeast cells in immobilised preparations. Various yeasts were used in this research: Saccharomyces cerevisiae cells that were rather sensitive to dehydration and had been aerobically grown in an ethanol-containing medium, a recombinant strain of S. cerevisiae grown in aerobic conditions which were completely non-resistant to dehydration and an anaerobically grown bakers' yeast strain S. cerevisiae, as well as a fairly resistant Pichia pastoris strain. Experiments performed showed that immobilisation of all these strains essentially increased their resistance to a dehydration-rehydration treatment. The increase of cells' viability (compared with control cells dehydrated in similar conditions) was from 30 to 60%. It is concluded that a new immobilisation method, which includes a dehydration stage, does not lead to an essential loss of yeast cell viability. Correspondingly, there is no risk of losing the biotechnological activities of immobilised preparations. The possibility of producing dry, active yeast preparations is shown, for those strains that are very sensitive to dehydration and which can be used in biotechnology in an immobilised form. Finally, the immobilisation approach can be used for the development of efficient methods for the storage of recombinant yeast strains.

Original languageEnglish
Pages (from-to)169-171
Number of pages3
JournalJournal of Biotechnology
Volume184
DOIs
Publication statusPublished - 20 Aug 2014
Externally publishedYes

Keywords

  • Dehydration
  • Immobilisation
  • Resistance
  • Viability
  • Yeast

Field of Science

  • 3.1 Basic medicine
  • 1.6 Biological sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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