Impact of biphasic calcium phosphate bioceramics on osteoporotic hip bone mineralization in vivo six months after implantation

Sandris Petronis, Janis Locs, Vita Zalite, Mara Pilmane, Andrejs Skagers, Ilze Salma, Girts Salms

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Calcium phosphate bone substitutes are successfully used for local recovery of osteoporotic bone and filling of bone defects. Previous studies reveled that biphasic calcium phosphate (BCP) show better bioactivity in compare to pure calcium phosphate (CaP) or hydroxyapatite (HAp). Also increased porosity of material promotes better bone tissue response. Aim of this experiment was to evaluate immunohistologically response of osteoporotic bone of experimental animal to implantation of granules with hydroxyapatite/β-tricalcium phosphate (HAp/β-TCP) ratio of 90/10. CaP was synthesized by aqueous precipitation technique from calcium hydroxide and phosphoric acid and sintered granules in size range from 1.0 to 1.4 mm were prepared with pore sizes around 200 nm. Nine female rabbits with induced osteoporosis were used in this experiment. Six animals in study group underwent implantation of BCP in hip bone defect and three animals in control group left without BCP implantation. After 6 months animals were euthanized, bone samples collected and proceeded for detection of bone activity and repair markers: osteocalcin (OC), osteopontin (OP) and osteoprotegerin (OPG). Controls demonstrated the presence of experimental bone osteoporosis. In experimental group bone showed partially resolved bioceramic material and in some samples new bone formation near the granules was observed. Increase of OC and OPG up to twice as to compare to control group were detected as well. Implantation of BCP HAp/βTCP 90/10 bioceramic in osteoporotic rabbit bone increases expression of OC and OPG indicating the activation of osteoblastogenesis and bone mineralization in vivo.

Original languageEnglish
Title of host publicationEngineering Materials and Tribology XXV
EditorsDagnija Loca
PublisherTrans Tech Publications Ltd
Number of pages5
ISBN (Print)9783035710724
Publication statusPublished - 2017
Event25th international Baltic Conference of Engineering Materials & Tribology: BaltMatTrib 2016 - Rīga, Latvia
Duration: 3 Nov 20164 Nov 2016
Conference number: 25

Publication series

NameKey Engineering Materials
Volume721 KEM
ISSN (Print)1013-9826
ISSN (Electronic)1662-9795


Conference25th international Baltic Conference of Engineering Materials & Tribology
Abbreviated titleBaltMatTrib 2016
Internet address


  • Bioactive compounds
  • Bone defect
  • Bone mineralization
  • Bone substitutes
  • Hydroxyapatite
  • Local osteoporosis treatment
  • Osteoblastogenesis
  • Osteocalcin
  • Osteopontin
  • Osteoprotegerin

Field of Science*

  • 3.1 Basic medicine
  • 3.2 Clinical medicine
  • 2.5 Materials engineering
  • 2.3 Mechanical engineering

Publication Type*

  • 3.1. Articles or chapters in proceedings/scientific books indexed in Web of Science and/or Scopus database


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